The Effect of Bentonite and Aflatoxin Absorbing Compounds on the Liver and Blood Parameters of Ross Breed Broiler Chickens
Subject Areas : Journal of Animal BiologyMahsa Moharreri 1 , Reza Vakili 2 * , Nafiseh Rahmanian Sharif Abad 3
1 - Department of Animal Science, Kashmar Branch, Islamic Azad University, Kashmar, Iran
2 - Department of Animal Science, Kashmar Branch, Islamic Azad University, Kashmar, Iran
3 - Department of Animal Science, Kashmar Branch, Islamic Azad University, Kashmar, Iran
Keywords: Bentonite, Aflatoxin, Liver damage, Blood parameters, Broilers,
Abstract :
The purpose of this research was to investigate the effects of bentonite and some aflatoxin absorbing compounds on the liver and blood parameters in Ross broiler chickens. The experiment was conducted in the form of a completely randomized design using 280 one-day-old broilers in 7 treatments and 4 replications. The experimental treatments included 7 groups including the control sample and the sample containing corn contaminated with aflatoxin and treatments with different percentages of bentonite and other compounds. In three phases of 10, 24, and 42 days, one sample was randomly selected from each treatment, slaughtered, and blood sampling was done. Then, the level of liver enzymes, cholesterol, triglyceride, uric acid, and glucose were measured by an auto-analyzer and the livers were checked for damage. Results showed that the enzyme aspartate aminotransferase and trans amino and glucose had a significant difference (p < 0.05). No significant difference was observed in the factors of cholesterol, triglycerides, and uric acid. Liver in The second treatment (infected corn) suffered from inflammation, bleeding and necrosis in all three sampling periods, and the rest of the treatments except one sample in the fifth treatment (1.5% bentonite and 5% copper sulfate) were healthy at 42 days. In general, in this research, the greatest effect of bleeding and necrosis of the liver was observed in the second treatment containing contaminated corn, while in other treatments with processed bentonite, the positive effect of absorbing toxins by bentonite was observed.
1. Abas, I., Bilal, T., Eseceli, H. 2011. The effect of organic acid, zeolite, or their combination on performance, some serum indices, and ileum pH values in broilers fed with different phosphorus. Turkish Journal of Veterinary and Animal Sciences, 35:337-344.
2. Abdelhamid, A.M., Salem, M.F.I., Mehrim, A.I., ElSharawy, M.A.M. 2007. Nutritious attempts to detoxify aflatoxin diets of tilapia fish: 1. Fish performance, feed and nutrients utilization, organs indices, residues and blood parameters. Egyptian Journal of Nutrition and Feeds, 10:205-223.
3. Azimi, J., Karimi Torshizi, M.A., Allameh, A. 2013a. Comparison of effectiveness of some mycotoxin absorbents on alteration of biochemical and hematological parameters in broiler chickens. Journal of Animal Science Research, 22(3):49-62. (In Persian).
4. Azimi, J., Karimi Torshizi, M.A., Allameh, A., Ahari, H. 2013b. Effect of adding two commercial absorbent materials and natural zeolite in feeds contaminated with aflatoxin B1 on broiler performance and immune system. Iranian Journal of Animal Science Research, 4(4):292-297. (In Persian).
5. Azizpour, A., Moghadam, N. 2015. Assessment of serum biochemical parameters and pathological changes in broilers with chronic aflatoxicosis fed glucomannan-containing yeast product (Mycosorb) and sodium bentonite. Bulletin of the Veterinary Institute in Puławy, 59:205-211.
6. Bagherzadeh Kasmani, F., Karimi Torshizi, M.A., Allameh, A.A., Sharitmadari, F. 2012. A novel aflatoxin binding Bacillus probiotic: performance, serum biochemistry and immunological parameters in Japanese quail. Poultry Science, 91:1846-1853.
7. Barati, M., Chamani, M., Mousavi, S. N., Hoseini, S.A., Taj Abadi Ebrahimi, M. 2018. Effects of biological and mineral compounds in aflatoxin-contaminated diets on blood parameters and immune response of broiler chickens. Journal of Applied Animal Research, 46:707-713.
8. Bovo, F., Franco, L.T., Kobashigawa, E., Rottinghaus, G.E., Ledoux, D.R, Oliveira, C.A.F. 2015. Efficacy of beer fermentation residue containing Saccharomyces cerevisiae cells for ameliorating aflatoxicosis in broilers. Poultry Science, 94:934-942.
9. Cheema, M., Qureshi, M., Havenstein. G. 2003. A comparison of the immune response of a 2001 commercial broiler with a 1957 random bred broiler strain when fed representative 1957 and 2001 broiler diets. Poultry Science, 82:1519-1529.
10. Chen, X., Horn, N., Applegate, T.J. 2014. Efficiency of hydrated sodium calcium aluminosilicate to ameliorate the adverse effects of graded levels of aflatoxin B1 in broiler chicks. Poultry Science, 93:2037-2047.
11. Haskard, C.A., El-Nezami, H.S., Kankaanpaa, P.E., Salminen, S., Ahokas, J.T. 2001. Surface binding of aflatoxin B1 by lactic acid bacteria. Applied and Environmental Microbiology, 67:3086-3091.
12. Khanian, M., Karimi Torshizi, M.A., Allameh, A. 2019. Alleviation of aflatoxin-related oxidative damage to liver and improvement of growth performance in broiler chickens consumed Lactobacillus plantarum 299v for entire growth period. Toxicon, 158:57–62.
13. Mahmoodtbar, A., Karimi Torshizi, M.A., Sharafi, M., Mojgani, N. 2018. The effect of some poultry probiotics produced in Iran on performance parameters, economic indices and small intestinal morphology of broilers. Iranian Journal of Animal Science, 49(3):415-425.
14. Mahmoodtabar, A., Karimi Torshizi, M.A., Sharafi, M., Mojgani, N. 2017. Comparing the effects of antibiotic growth promoter, some Iranian probiotics and similar imported products on performance, economic indicators and small intestinal morphology of broilers. Iranian Journal of Animal Science, 48(3):321-334.
15. Manafi, M. 2018. Impact of application of natural toxin binder on performance, humoral immune response, cecal microbial population and changes in small intestine morphology of broilers fed with diet contaminated with aflatoxin B1. Journal of Veterinary Research, 73(3):273-282.
16. Manafi, M. (2012). Counteracting effect of high grade sodium bentonite during aflatoxicosis in broilers. Journal of Agricultural Science and Technology, 14:539-547.
17. Osweiler, G.D., Jagannatha, S., Trampel, D.W., Imerman, P.M., Ensley, S.M., Yoon, I., Moore D.T. 2010. Evaluation of XPC and prototypes on aflatoxin-challenged broilers. Poultry Science, 89:1887-1893.
18. Ramos, A.J., Hernandez, E. 1997. Prevention of aflatoxicosis in farm animals by means of hydrated sodium calcium aluminosilicate addition to feedstuffs: a review. Animal Feed Science and Technology, 65:197-206.
19. Rawal, S., Kim, J.E., Coulombe, J.R. 2010. Aflatoxin B1 in poultry: Toxicology, metabolism and prevention. Research in Veterinary Science, 89:325-331.
20. Rehulka, J., Minarik, B., Adamec, V., Rehulkova, E. 2005. Investigations of physiological and pathological levels of total plasma protein in rainbow trout, Oncorhynchus mykiss (Walbaum). Aqua Research, 36:22-32.
21. Safari, M.H., Shams Shargh, M., Amini, A., Tatyar, A. 2014. Effects of different levels of natural glauconite and zeolite on performance, tibia bone characteristics and blood parameters of broiler chicken. Animal Science Journal, (Pajouhesh-Va-Sazandegi), 105:167-178. (In Persian).
22. Shi, Y.H., Xu, Z.R., Feng, J.L., Wang, C.Z. 2006. Efficacy of modified montmorillonite nanocomposite to reduce the toxicity of aflatoxin in broiler chicks. Animal Feed Science and Technology, 129:138-148.
23. Taherpour, K., Moravej, H., Shivazad, M., Adibmoradi, M., Yakhchali, B. 2009. Effects of dietary probiotic, prebiotic and butyric acid glycerides on performance and serum composition in broiler chickens. African Journal of Biotechnology, 8:2329-2334
24. Yunus, A.W., Razzazi-Fazeli, E., Bohm, J. 2011. Aflatoxin B1 in affecting broiler’s performance, immunity, and gastrointestinal tract: A review of history and contemporary issues. Toxins, 3:566-590.