اثر بنتونیت و ترکیبات جاذب آفلاتوکسین بر کبد و فراسنجههای خونی جوجههای گوشتی نژاد راس
محورهای موضوعی : فصلنامه زیست شناسی جانوریمهسا محرری 1 , رضا وکیلی 2 * , نفیسه رحمانیان شریف آباد 3
1 - گروه علوم دامی، واحد کاشمر، دانشگاه آزاد اسلامی، کاشمر، ایران
2 - گروه علوم دامی، واحد کاشمر، دانشگاه آزاد اسلامی، کاشمر، ایران
3 - گروه علوم دامی، واحد کاشمر، دانشگاه آزاد اسلامی، کاشمر، ایران
کلید واژه: بنتونیت, آفلاتوکسین, آسیب های کبدی, فراسنجه های خونی, جوجههای گوشتی,
چکیده مقاله :
هدف از این تحقیق، بررسی اثرات بنتونیت و برخی ترکیبات جاذب آفلاتوکسین بر کبد و فراسنجههای خونی در جوجههای گوشتی نژاد راس بود. آزمایش در قالب یک طرح به صورت کاملا تصادفی و با استفاده از 28٠ قطعه جوجه گوشتی یک روزه در هفت تیمار و چهار تکرار انجام شد. تیمارهای آزمایشی شامل 7 گروه شامل نمونه شاهد و نمونه حاوی ذرت آلوده به آفلاتوکسین و تیمارهایی با درصدهای مختلف بنتونیت و سایر ترکیبات بود. در سه فاز 11 و 24 و 42 روزگی از هر تیمار یک نمونه به صورت تصادفی انتخاب، کشتار و خونگیری و نمونهگیری از کبد انجام شد. سپس میزان آنزیم‌های کبدی و میزان کلسترول و تری گلیسرید و اوریک اسید و گلوکز توسط دستگاه اتوانالایزر اندازه گیری و کبدها از لحاظ داشتن ضایعات بررسی شدند. نتایج نشان داد که آانزیم آسپارات امینوترانسفراز و ترانسآامینو و گلوکز اختلاف معنی‌داری داشتند (05/0 p <). در فاکتورهای کلسترول و تری‌گلیسرید و اوریک اسید اختلاف معنی‌داری مشاهده نشد و همین طور بافت کبد در تیمار دوم (ذرت آلوده) در هر سه دوره نمونهگیری دچار التهاب و خونریزی و نکروز شده و بقیه تیمارها به جز یک نمونه در تیمار پنجم (5/1 درصد بنتونیت و 5 درصد سولفات مس) در 42 روزگی سالم بودند. بطور کلی در این تحقیق بیشترین اثر خونریزی و نکروز کبد در تیمار دوم حاوی ذرت آلوده مشاهده شد که در سایر تیمارهای دارای بنتونیت فرآوری شده اثر‌مثبت جذب سموم توسط بنتونیت مشاهده شد.
The purpose of this research was to investigate the effects of bentonite and some aflatoxin absorbing compounds on the liver and blood parameters in Ross broiler chickens. The experiment was conducted in the form of a completely randomized design using 280 one-day-old broilers in 7 treatments and 4 replications. The experimental treatments included 7 groups including the control sample and the sample containing corn contaminated with aflatoxin and treatments with different percentages of bentonite and other compounds. In three phases of 10, 24, and 42 days, one sample was randomly selected from each treatment, slaughtered, and blood sampling was done. Then, the level of liver enzymes, cholesterol, triglyceride, uric acid, and glucose were measured by an auto-analyzer and the livers were checked for damage. Results showed that the enzyme aspartate aminotransferase and trans amino and glucose had a significant difference (p < 0.05). No significant difference was observed in the factors of cholesterol, triglycerides, and uric acid. Liver in The second treatment (infected corn) suffered from inflammation, bleeding and necrosis in all three sampling periods, and the rest of the treatments except one sample in the fifth treatment (1.5% bentonite and 5% copper sulfate) were healthy at 42 days. In general, in this research, the greatest effect of bleeding and necrosis of the liver was observed in the second treatment containing contaminated corn, while in other treatments with processed bentonite, the positive effect of absorbing toxins by bentonite was observed.
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