The Effect of Diazepam on the Number of Primary Spermatocytes and Spermatid Cells in Male Rats
Subject Areas : Journal of Animal BiologyAmir bagher Eilkhani 1 , Maryam Tehranipour 2 * , Saiede Zafarbalanejad 3
1 - Department of Biology, Islamic Azad University, Mashhad Branch, Mashhad, Iran
2 - Department of Biology, Islamic Azad University, Mashhad Branch, Mashhad, Iran
3 - Department of Biology, Islamic Azad University, Mashhad Branch, Mashhad, Iran
Keywords: Diazepam, Primary spermatocyte, Rat,
Abstract :
Diazepam, from the benzodiazepine family, has been widely used in recent years due to its sedative and pain-relieving effects. On the other hand, simultaneously with the use of such drugs, many disorders are seen in the process of spermatogenesis, so the purpose of this research is to investigate the effect of diazepam drug use on the number of primary spermatocytes and spermatids in male rats. In this experimental study, 30 male Wistar rats weighing 200-250 grams were selected and randomly divided into 5 groups of 6. The rats in the control group received physiological serum and the rats in the treatment groups received diazepam at doses of 2, 3, 4, and 5 mg/ml of body weight intraperitoneally for 14 days. After anesthetizing the animals, samples were taken from the testicles and a slide was prepared, and the number of primary spermatocytes, Sertoli spermatids and spermatogonia were counted. The data were analyzed using Anova statistical analysis at a significant level (p < 0.05). The results showed that the number of cells examined in the treatment group with a dose of 3 mg/ml compared to the control group decreased significantly (p < 0.05). Probably, the use of diazepam has reduced the proliferation and division of testicular cells by inhibiting the hypothalamus-pituitary-gonadal axis.
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