Effect of Stem Cells Containing TSP-1 Gene along with Letrazole on ROS and VEGF Levels in Polycystic Ovary Syndrome Induced by Estradiol Valproate in Female Wistar Rat Model

Subject Areas : Journal of Animal Biology

masoumeh Rahimi ¹ (Effect of Stem Cells Containing TSP-1 Gene along with Letrazole on ROS and VEGF Levels in Polycystic Ovary Syndrome Induced by Estradiol Valproate in Female Wistar Rat Model)
maryam bananaj ² (Effect of Stem Cells Containing TSP-1 Gene along with Letrazole on ROS and VEGF Levels in Polycystic Ovary Syndrome Induced by Estradiol Valproate in Female Wistar Rat Model)
Ramin Hajikhani ³ (Effect of Stem Cells Containing TSP-1 Gene along with Letrazole on ROS and VEGF Levels in Polycystic Ovary Syndrome Induced by Estradiol Valproate in Female Wistar Rat Model)
maryam akhavantaheri ⁴ (Department of Histology and Pathology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran)

Received: 2022-02-16 Accepted : 2023-08-02 Published : 2024-03-05

Keywords: Polycystic ovary syndrome, Endometrial cell, TSP-1, letrazole,

Abstract :

Polycystic ovary syndrome (PCOS) is a complex genetic, endocrine and metabolic heterogeneous disorder and the most common endocrine disease and metabolic disorder in children of reproductive age and the most important cause of infertility due to ovulation in women. There is a direct and close relationship between systemic and local inflammation and the rate of angiogenesis and PCOS. Stem TSP-1 and mice receiving letrazole and mice receiving concomitant TSP-stem cells and letrazazole were categorized and VEGF was measured by Real Time PCR and ROS by ELISA. Wistar female rats with polycystic ovary syndrome (PCOS) had a significant increase in VEGF gene expression and tissue ROS levels compared to the control group, which was significant. The results showed a significant decrease in VEGF gene expression and ROS levels compared with PCOS mice. The highest reduction was observed in letrrazole-receiving mice with TSP-1-containing stem cells compared to the other two treatments in both VEGF and ROS. ROS and VEGF levels are associated with increased ovarian masses, and increased ovarian masses with hypoxia, androgens, insulin, and AMH may increase VEGF levels.

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