Evaluation of LncRNA SNHG15 Expression Changes in the Tissues of Glioblastoma Multiforme Patients Using Real-Time PCR

Subject Areas : Journal of Animal Biology

Sahar Shakeri yekta ¹ , Elham Moslemi ² , Soyar Sari ³ , Fatemeh Roholla ⁴ , Hamidreza Kheiri ⁵

1 - Department of Molecular and Cellular Sciences, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University,Tehran, Iran
2 - Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Department of Molecular and Cellular Sciences, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University,Tehran, Iran
4 - Department of Molecular and Cellular Sciences, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University,Tehran, Iran
5 - Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran

Received: 2022-10-23 Accepted : 2022-11-24 Published : 2023-08-23

Keywords: Gene expression, Real-time PCR, Glioblastoma multiforme, SNHG15, LncRNA,

Abstract :

Glioblastoma is an aggressive malignant tumor of the brain and spinal cord. Several lines of evidence indicate an important oncogenic role of highly non-coding RNA (lncRNA) molecules in a variety of cancers including glioma, however, the tumorigenic function of lens in glioma remains largely unclear. Increasing evidence has shown that lncRNAs, including LncRNA SNHG15, play an important role in the pathophysiology of human diseases, especially in the pathogenesis and progression of cancers. In this study, 25 paraffin tissue blocks of patients with glioblastoma multiforme and tumor margin tissue were collected and RNA extracted. Synthesis of cDNA and analysis of SNHG15 expression was done by Real-Time PCR technique. ROC curve was drawn to check the biomarker value and statistical analysis was done by GraphPad Prism v.8.0.1. An increase in the expression of SNHG15 was recorded in the tissue samples of patients compared to the tissue of the tumor margin (p < 0.0001). There was a significant relationship in the expression of this gene in patients aged more than 50 years and less than 50 years (p = 0.6573), Frontal, Temporal, Parietal, Occipital, and Other locations (P value = 0.9802), survival in more than 12 months and less than 12 months (p = 0.5007) was not observed, only in male and female sex (p = 0.0001) was registered. The expression of LncRNA SNHG15 was recorded in the tumor tissue of patients with glioblastoma multiforme more than in the peripheral tumor tissue. By examining the ROC curve, it is possible that LncRNA SNHG15 can be proposed as a biomarker, but it needs more studies.

References:

1. Abdul K.U., Houweling M., Svensson F., Narayan R.S., Cornelissen F.M., Küçükosmanoglu A., Metzakopian E., Watts C., Bailey D., Wurdinger T., Westerman B.A. 2018. WINDOW consortium: a path towards increased therapy efficacy against glioblastoma. Drug Resistance Updates, 40:17-24.


2. Arcuri C., Fioretti B., Bianchi R., Mecca C. Tubaro C., Beccari T., Franciolini F., Giambanco I., Donato R. 2017. Microglia-glioma cross-talk: a two-way approach to new strategies against glioma. Frontiers in Bioscience-Landmark, 22(2):268-309.


3. Banu Z. 2019. Glioblastoma multiforme: a Review of its Pathogenesis and Treatment.  Res. J. Pharm, 9:7-12.


4. Chen X., Fan X., Zhao C., Zhao Z., Hu L., Wang D., Wang R., Fang Z. 2020. Molecular subtyping of glioblastoma based on immune-related genes for prognosis. Scientific Reports, 10(1):1-12.


5. Cui H.X., Zhang M.Y., Liu K., Liu J., Zhang Z.L., Fu L. 2018. LncRNA SNHG15 promotes proliferation and migration of lung cancer via targeting microRNA-211-3p. European Review in Medical Pharmacological Sciences, 22(20):6838-6844.


6. Dubois L.G., Campanati L., Righy C., D’Andrea-Meira I., Spohr T.C.L., Porto-Carreiro I., Pereira C.M., Balca-Silva J., Kahn S.A., DosSantos M.F., Oliveira M.D.A.R. 2014. Gliomas and the vascular fragility of the blood brain barrier. Frontiers in Cellular Neuroscience. 8:418.


7. Gi T., Sato Y., Tokumitsu T., Yamashita A., Moriguchi-Goto S., Takeshima H., Sato S., Asada Y. 2017. Microvascular proliferation of brain metastases mimics glioblastomas in squash cytology. Cytopathology, 28(3):228-234.


8. Huang J., Chen Y.X., Zhang B. 2020. IGF2-AS affects the prognosis and metastasis of gastric adenocarcinoma via acting as a ceRNA of miR-503 to regulate SHOX2. Gastric Cancer, 23(1):23-38.


9. Huang Y., Xu Y., Lu Y., Zhu S., Guo Y., Sun C., Xu L., Chen X., Zhao Y., Yu B., Yang Y. 2019. lncRNA Gm10451 regulates PTIP to facilitate iPSCs-derived β-like cell differentiation by targeting miR-338-3p as a ceRNA. Biomaterials, 216: 119266.


10. Kheiri Manjili H., Ma’mani L., Tavaddod S., Mashhadikhan M., Shafiee A., Naderi-Manesh H. 2016. D, L-sulforaphane loaded Fe3O4@ gold core shell nanoparticles: a potential sulforaphane delivery system. PloS one, 11(3):0151344.


11. Kong Q., Qiu M. 2018. Long noncoding RNA SNHG15 promotes human breast cancer proliferation, migration and invasion by sponging miR-211-3p. Biochemical and Biophysical Research Communications, 

495(2):1594-1600.

12. Li Q., Aishwarya S., Li J.P., Pan D.X., Shi J.P. 2022. Gene Expression Profiling of Glioblastoma to Recognize Potential Biomarker Candidates. Frontiers in Genetics, 13:832742.


13. Li Z., Zhang J., Zheng H., Li C., Xiong J., Wang W., Bao H., Jin H., Liang P. 2019. Modulating lncRNA SNHG15/CDK6/miR-627 circuit by palbociclib, overcomes temozolomide resistance and reduces M2-polarization of glioma associated microglia in glioblastoma multiforme. Journal of Experimental and Clinical Cancer Research, 38(1):1-13.


14. Liu Y., Li J., Li F., Li M., Shao Y., Wu L. 2019. SNHG15 functions as a tumor suppressor in thyroid cancer. Journal of Cellular Biochemistry, 120(4):6120-6126.


15. Ma Y., Xue Y., Liu X., Qu C., Cai H., Wang P., Li Z., Li Z., Liu Y. 2017. SNHG15 affects the growth of glioma microvascular endothelial cells by negatively regulating miR-153. Oncology Reports, 38(5):3265-3277.


16. Mamelak A.N., Jacoby D.B. 2007. Targeted delivery of antitumoral therapy to glioma and other malignancies with synthetic chlorotoxin (TM-601). Expert Opinion on Drug Delivery, 4(2):175-186.


17. Müller Bark J., Kulasinghe A., Chua B., Day B.W., Punyadeera C. 2020. Circulating biomarkers in patients with glioblastoma. British Journal of Cancer, 122(3):295-305.


18. Pi J., Liu J., Zhuang T., Zhang L., Sun H., Chen X., Zhao Q., Kuang Y., Peng S., Zhou X., Yu Z. 2018. Elevated expression of miR302-367 in endothelial cells inhibits developmental angiogenesis via CDC42/CCND1 mediated signaling pathways. Theranostics, 8(6):1511.


19. Poon C.C., Sarkar S., Yong V.W., Kelly J.J. 2017. Glioblastoma-associated microglia and macrophages: targets for therapies to improve prognosis. Brain. 140(6):1548-1560.


20. Sarkaria J.N., Hu L.S., Parney I.F., Pafundi D.H., Brinkmann D.H., Laack N.N., Giannini C., Burns T.C., Kizilbash S.H., Laramy J.K., Swanson K.R. 2018. Is the blood–brain barrier really disrupted in all glioblastomas? A critical assessment of existing clinical data. Neuro-Oncology, 20(2):184-191.


21. Shuai Y., Ma Z., Lu J., Feng J. 2020. LncRNA SNHG15: a new budding star in human cancers. Cell Proliferation, 53(1): 12716.


22. Sun X., Bai Y., Yang C., Hu S., Hou Z., Wang G. 2019. Retracted Article: Long noncoding RNA SNHG15 enhances the development of colorectal carcinoma via functioning as a ceRNA through miR-141/SIRT1/Wnt/β-catenin axis. Artificial cells. Nanomedicine, and Biotechnology, 47(1):2536-2544.


23. Wang C.J., Zhu C.C., Xu J., Wang M., Zhao W.Y., Liu Q., Zhao G., Zhang Z.Z. 2019. The lncRNA UCA1 promotes proliferation, migration, immune escape and inhibits apoptosis in gastric cancer by sponging anti-tumor miRNAs. Molecular Cancer, 18(1):1-12.


24. Wu D.M., Wang S., Wen X., Han X.R., Wang Y.J., Shen M., Fan S.H., Zhang Z.F., Shan Q., Li M.Q., Hu B. 2018. LncRNA SNHG15 acts as a ceRNA to regulate YAP1-Hippo signaling pathway by sponging miR-200a-3p in papillary thyroid carcinoma. Cell Death and Disease, 9(10):1-15.


25. Zhang J.H., Wei H.W., Yang H.G. 2016. Long noncoding RNA SNHG15, a potential prognostic biomarker for hepatocellular carcinoma. European Review in Medicine and Pharmacological Sciences, 20(9):1720-1724.