The Effects of Chitosan/Polyvinilalchol Nanofibers Scaffold on Skin Burns in Adult Male NMRI Mouse
Subject Areas : Journal of Animal Biology
لیلا
ریاحی
1
(گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران)
نسیم
حیاتی رودباری
2
(گروه زیست شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران)
سهیلا
سلحشور کردستانی
3
(گروه مهندسی پزشکی، دانشگاه صنعتی امیرکبیر، تهران، ایران)
کاظم
پریور
4
(گروه مهندسی پزشکی، دانشگاه صنعتی امیرکبیر، تهران، ایران)
Keywords: Wound healing, Chitosan, Electrospinning, Polyvinylalcohol, nanofibrous scaffolds,
Abstract :
To repair the wounds of burns a wide range of research including the use of bio-scaffolds due to ability to achieve the desired characteristics has been done. According to favorable biological properties of chitosan such as biocompatibility, antimicrobial properties and the ability to improve wound healing in biomedical applications, and also due to biocompatibility and lack of stimulation of the immune system of polyvinyl alcohol, the authors decided to investigate the in-vivo effect of Nanofiber scaffolds on the mix of these two polymers on damaged skin.30 NMRI male mice were randomly divided in to a control group and three experimental groups (Scaffolding chitosan: polyvinylalcohol ratio of 0.100, 20.80, 30.70).Under sterile conditions and anesthesia, a 1 cm in diameter and full thickness skin wounds was created on the back of each mouse. Electrospinning nanofiber scaffolds were applied as wound dressing to cover the burn. The burn area was sterile every day and scaffolding was dressing on the wound. After 21 days histological studies have been done on the wound. The results showeda significant meaningful reduction in wound diameter in the experimental group in comparison to control group(p Also increase in the rate of wound shrinkage has been seen in samples containing scaffolds compared to control samples.