The effect of methanol and salicylic acid foliar application on the physiological and antioxidant properties of rapeseed (Brassica napus L.) under low irrigation conditions

Subject Areas : Agroecology Journal

Samaneh Safajo ¹ (Ph.D Student of Agronomy, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran)
Ali Faramarzi ² (Department of Agronomy and Plant Breeding, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran.)
Jalil Ajali ³ (- Department of Agronomy and Plant Breeding, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran.)
Mehrdad Abdi ⁴ (Department of Agronomy and Plant Breeding, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran.)
Mehdi Oraei ⁵ (Department of Horticultural Sciences, Faculty of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran)

Received: 2023-12-15 Accepted : 2023-12-15 Published : 2021-11-22

Keywords: Salicylic acid, Methanol, Rapeseed, Proline, Dehydration Stress, Antioxidant enzymes,

Abstract :

In order to investigate the effect of methanol and salicylic acid foliar application on the activity of antioxidant enzymes (catalase, guaiacol peroxidase and ascorbate peroxidase), soluble carbohydrates and proline content of rapeseed (Brassica napus L) in dehydration stress condition, an split-factorial experiment was conducted based on randomized complete block design with three replications in two years (2017 and 2018) in Safiabad Agricultural Research and Training Center of Dezful. The treatments of dehydration stress included three levels (70, 140 and 210 mm evaporation) as main plots and combination of treatments of salicylic acid on three level (control, 100 and 20 μmol) and methanol foliar application on three levels (control, 10 and 20 % Volume) as subplots. The results of analysis of variance showed that the effect of dehydration stress on the activity of catalase, guaiacol peroxidase and ascorbate peroxidase enzymes as well as soluble carbohydrates and proline content was significant. Further more, foliar application of methanol and salicylic acid on measured traits was significant Mean comparison showed that dehydration stress has led to increase activity of antioxidant enzymes as well as soluble carbohydrates and proline content. Finally, foliar application of methanol and salicylic acid has reduced the activity of antioxidant enzymes and increased the content of soluble carbohydrates and proline in rapeseed.