Background: Obesity is the result of accumulation of excess adipose tissue due to an imbalance between food intake and energy intake. Exercise can play an effective role in reducing adipose tissue due to increased energy consumption. The aim of this study was to investigate the effect of high intensity training (HIIT) on adiponectin levels in adipose tissue in rats on a high fat diet.Methods: For this purpose, 32 rats with a lifespan of 8 weeks were randomly divided into 3 groups: obese and HIIT, obese without HIIT and normal weight. The HIIT training program was performed for eight weeks. At the end of the training period, the rats were anesthetized. The subcutaneous adipose tissue of the animal was also sampled and qRT-PCR analysis was performed to evaluate the expression of adiponectin gene.Results: The results showed that adiponectin gene expression decreases under the influence of obesity. But with HIIT training and reduced obesity, its expression increases.Conclusion: In general, the results of this study showed that HIIT training can reduce obesity, increase adiponectin gene expression in animals with high fat diet. Manuscript profile