Among plant growth regulators, Polyamines (PA) have a major role in diverse plant physiological responses throughout vegetative to reproductive phase and senescence. The present study was conducted to study the impact of polyamine on different physiological and biochemical aspects of hydroponic grown rose (cv. 'Dulce Vita') plants. Putrescine (Put) (at 1, 2, 3 mM), Spermidine (Spd) (at 0.5, 1, 1.5 mM) and Spermine (Spm) (at 1, 2, 4 mM) were sprayed on rose plants during growth and subsequently, different aspects of vegetative growth, flower quality, mineral uptake and plant response to some biochemical responses were studied. Foliar application of Spd at 1.5 mM concentration caused the highest flower stem length and flower bud size. Vase life was affected by PA application, in which, Spd had the greatest beneficial outcome. Absorption and accumulation of the studied macro- and micro-nutrient content in leaves were also influenced by PA treatment. ACC synthase and chlorophyllase activity in leaves of cut flowers harvested from PA treated plants was affected, in which 4mM Spm treatment lead to the least. Although malondialdehyde production in flower stem leaves of ‘Dolce Vita’ rose increased during postharvest life as flowers aged, but the increment was retarded by PA application. Manuscript profile