Background: This study pursues two goals: first, presenting Persian translation and cultural adaptation of Eating Disorders Screen for Athletes (EDSA) in accordance with an international standard method, and second, studying factor structure and psychometric properties of EDSA. Methods: A total of 439 athletes aged 18 years and older from various sports all around the country participated in this research. A confirmatory factor analysis was utilized to present the validation of the structure. Internal consistency was also examined using Cronbach's alpha coefficient. Concurrent validity was evaluated using the zero correlation between EDSA and the Eating Disorder Examination Questionnaire (EDE-Q). Receptor Operating Characteristic (ROC) curve analysis based on gender and distinct performance analysis were used to predict the grouping of athletes based on the EDSA cut-off point (3.33 as the optimal cut for men and women) from the benchmark test. The Persian version of the EDE-Q in athletes has a similar factor structure to the English one. The questionnaire's reliability obtained by Cronbach's alpha was 0.744. The correlation between the scores of this questionnaire and the EDE_Q was confirmed. Diagnostic validity for classification of male and female athletes with low and high eating disorders has a significance level of P <0.001, and a cut-off score of 3.25 on EDSA presented an optimal exchange of sensitivity in return for sensitivity in genders. .Results: CStandardized factor loadings ranged between .43 and .81 at the significance level of p = 0.001. Conclusions: The present research indicates the successful translation, development and validation of Persian EDSA as a screening tool to identify male and female athletes at risk for eating disorders. The Persian version of EDSA can be confidently used in Persian language research and applied fields. Manuscript profile