Investigation on variation of alkaloids content in different parts of Physalis divaricata D. Don. From Shahrekord region

Subject Areas : Phytochemistry

Elham Moallem ¹ , Prof. Abdollah Ghasemi Pirbalouti ² , Iraj Mehregan ³ , Taher Nejhad Sattari ⁴ , Alireza Iranbakhsh ⁵

1 - Department of Plant Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Research Center for Medicinal Plants, IAU., Shahrekord Branch, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2017-08-15 Accepted : 2018-09-24 Published : 2018-05-22

Keywords: HPLC, atropine, Shahrekord, Physalis divaricate D, Physalin B, Scopolamin,

Abstract :

Physalis divaricata D. Don.is a medicinal plant belonging to Solanaceae family. It consists of different tropane alkaloids, especially atropine, scopolamine, and physalin. In this study, P. divaricata was cultivated at greenhouse conditions in the field experimental of Islamic Azad University of Shahrekord. So various parts of plant, including root, stem, leaf, fruit, and flower at the late flowering stage were harvested in Spring 2015 and then the ethanolic extracts of the samples were obtained by soxhlet extraction method. The contents of atropine, scopolamineand physalin B were measured by calculating the curve of HPLC with three replications. The comparison results at the 5% level indicated that the fruits extract had the highest content of physalin B, the highest content of atropine was reported from the leaves extract and the highest content of scopolamine was reported from the flower extract. In conclusion, the main source of the secondary metabolites seemed to be a difference in type parts of the herb. 

References:

1. Alaghemand, A., Ghorbanpour, M., Eradatmand, Asli, D. and Moghaddasian, B. 2013. Influence of urea fertilization on tropane alkaloids content of henbane (Hyoscyamus niger L.) under hydroponic culture conditions. Journal Advances in Environmental Biology, 7: 307-318.


2. Alves, M.N., Sartoratto, A. and Trigo, J.R. 2007. Scopolamine in Brugmansia suaveolens (Solanaceae): Defense, allocation, costs, and induced response. Journal of Chemical Ecology, 33: 297–309.


3. Antoun, M.D., Abramson, D., Tyson, R.L., Chang, C.J., McLaughlin, J.L., Peck, G. and Cassady, J.M. 1981. Potential antitumor agents XVII Physalin B and 25, 26-epidihydrophysalin C from Witheringia coccoloboides. Journal Natural Products, 44: 579-585.


4. Ashtiania, F. and Sefidkon, F. 2001. Tropane alkaloids of Atropa belladonna  L. and Atropa acuminata  Royle ex Miers plants.  Journal of Medicinal Plants Research, 5: 6515- 6522 .


5. Azemi, M. E., Mosaddegh, M., Cheraghali, A. M. and Namjooyan, F. 2006. Isolation and identification of calystegines in root cultures of four Physalis species. Iranian Journal Pharmaceutical Research, 5: 69-72.


6. Bahmnazadegan. A., Sefidkon, F., Sonboli,A. and Jaimand ,K. 2008. Extraction and determination of tropane alkaloids, hyoscyamine and scopolamine, form different parts of Hyoscyamus reticulatus L. and Hyoscyamus pusillus L. Pajouhesh va Sazandegi, 79: 145 - 53.


7. Basey, K., McGaw, B. A. and Woolley, J.G. 1992. Phygrine, an alkaloid from Physalisspecies. Phytochemistry, 31: 4173-4176.


8. Bastos, G. N., Silveira, A. J., Salgadod, C. G., Picanco-Diniz, D. L. and Nascimento, J. L. 2008. Physalis extract exerts anti-inflammatory effects in rats by inhibiting different pathways. Journal of Ethnopharmacology, 118: 246-5.


9. Butnaru, C., Butnaru, E., Vlase, L. and Laza, M. 2010. Determination of Scopoletin in Physalis alkekengy and Solanum dulcamara by high- performance liquid chromatography. Farmacia, 58(6): 711-717.


10. Chiang, H.C., Jaw, S.M., Chen, C.F. and Kan, W.S. 1992. Antitumor agent, physalin F from Physalis angulata L. Anticancer Research, 12: 837-844.


11. Dehghan, E., Shahriari Ahmadi, F., Ghotbi Ravandi, E., Reed, D.W. and Covello, P.S. 2013. An atypical pattern of accumulation of scopolamine and other tropane alkaloids and expression of alkaloid pathway genes in Hyoscyamus senecionis. Journal Plant Physiology and Biochemistry, 70: 188-195.


12. Demeyer, K. and Dejaegere, R. 1992. Effect of the nitrogen form used in the growth medium (NO^-3 , NH⁺⁴) on alkaloid production in Datura stramonium L. Plant and Soil, 147: 79- 86.


13. Dialmaghani, K., Karvari-Nejad, R., Fahimi,H. and Hekmat-shoar, H. 2007. Comparison of tropane alkaloids content of Hyoscyamus reticulatus .L and Hyoscyamus arachnoideus Pojark at different growth stages. Journal of Science Islamic Azad University, 66: 51 – 61.


14. Dialmaghani, K., Kharvari-Nejad, R., Fahimi1 , H. and Hekmat-shoar, H. 2006. Extraction and determination of tropan alkaloids, hyoscyamine and scopolamine, from different parts of Hyoscyamus pusillus L. in different stages of plant growth. Journal of Medicinal Plants, 22: 1-10.


15. El Sheikh, M. O. A., El Hassan, G. M., Tayeb Abdel Hafeez, A. R., Abdalla, A. A. and Antoun M. D. 1982. Studies on Sudanese medicinal plants I I I: Indigenous Hyoscyamus muticus as commercial source hyoscyamine. Planta Medica, 53(4): 349-354.


16. Fattahi, F., Shojaeiyan, A., Ashkari, H., Naghdi Badi, H., Ayyari, M., Mokhtassi Bidgoli, A. and Palazon, B. 2015. Quantitative assessment of Hyoscyamine and Scopolamine tropan alkaloids in root and shoot of Hyoscyamaus spp. Iranian Journal of Medicinal Plants, 14: 105-114.


17. Grynkiewicz, G. and Gadzikowska, M. 2008. Tropane alkaloids as medicinally useful natural products and their synthetic derivatives as new drugs. Pharmacological Reports, 60: 439–463.


18. Hashimoto, T., Tamaki, K., Suzuki, K. and Yamada, Y. 1998. Molecular cloning of plant spermidine synthases. Plant and Cell Physiology, 39:73–79.


19. Hoshani, M., Aghdasi, M. A. and Mohseni, M. 2012. Inhibitory effect of Physalis alkekengi extracts in different phenological stages on xanthine oxidase activity. Physiology and Pharmacology, 16 (3): 300-309.


20. Iranbakhsh, A., Oshaghi, M. A. and Majd, A. 2006. Distribution of Atropane and Scopolamine in Different Organs and Stages of Development in Datura stramonium L. (Solanaceae) Structure and Ultra structure of biosynthesizing Cells. Acta Biologica Cracoviansia, 48(1): 13-18.


21. Ismail, N. and Alam, M. 2001. A novel cytotoxic flavonoid glycoside from Physalis angulata. Fitoterapia, 72: 676-689.


22. Jualang Azlan, G., Marziah, M., Radzali, M.  and Johari, R. 2005. Accumulation of Physalin in cells and tissues of Physalis minima L. Wocmap III, 2: 53- 59.


23. Kamada, H., Okamura, N., Satake, M., Harada, H. and Shimomura, K. 1986. Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Reports, 5: 239-242.


24. Khatamsaz, M. 1998. Flora of Iran (Solanaceae) (1^st Edition), Forage and Range- land Research Institute Publication, Tehran, Iran, 55 pp.


25. Kitamura, Y., Sato, M. and Miura, H. 1992. Differences of atropine esterase activity between intact roots and cultured roots of various tropane alkaloid producing plants. Phytochemistry, 31(4): 1191-1194.


26. Lijing, L. 2012. Study on callus induction and regeneration of Physalis alkekengi. Agricultural Biotechnological, 6: 39-41.


27. Marín-Sáez, J., Romero-González, R., Frenich, A.G. and Egea-González, F.J. 2018. Screening of drugs and homeopathic products from Atropa belladonna seed extracts: tropane alkaloids determination and untargeted analysis. Drug Testing and Analysis, DOI:10.1002/dta.2416.


28. Masrournia, M., Es’haghi, Z. and Amini, M. 2011. Liquid chromatographic determination of scopolamine in hair with suspended drop liquid phase microextraction technique. American Journal of Analytical Chemistry, 2: 232-235.


29. Moghaddam, M., Ghasemi Pirbalouti, A. and Farhadi, N. 2018. Seasonal variation in Juniperus polycarpos var. turcomanica essential oil from northeast of Iran. Journal of Essential Oil Research, 30(3): 225-231.


30. Nyman, S., Soderman, A. and Simola, L.K. 1993. Metabolism of Arginine and ornithine in suspension-cultured cells and Aseptic roots of interact plants of Atropa belladonna. Journal of Experimental Botany, 44 (262): 869-877.


31. Oksman- Caldenty, K. M., Vrurela, H., Straub, A. and Hiltunen, R. 1987. Variation in the tropane alkaloid content of Hyoscyamus muticus plant and cell culture clones. Planta Medica, 53(4): 349- 354.


32. Omidi, M., Koohzadi, F., Solouki, M., Taghizad Farid, R. and Alizadeh H. 2011. Comparison of morphinan alkaloids during different stages of growth in the medicinal plant opium poppy (Papaver somniferum L.).  Journal of Giahan Daroee, 44: 144-149.


33. Perez-Castorena, A.-L., Luna, M., Martínez, M. and Maldonado, E. 2012. New sucrose esters from the fruits of Physalis solanaceus. Carbohydrate Research, 352: 211-214.


34. Pinto, J. G. A., Hornby, K. R., Jones, D. G. and Murphy, K. M. 2010. Developmental changes in GABAergic mechanisms in human visual cortex across the lifespan. Front Cell Neuroscience, 4: 16-22.


35. Qiu,  L., Zhao, F., Jiang, Z.-H., Chen, L.-X., Zhao, Q., Liu, H.-X., Yao, X.-S. and Qiu,  F. 2008. Steroids and flavonoids from Physalis alkekengi var. franchetii and their inhibitory effects on nitric oxide production. Journal of Natural Products, 71: 642-646.


36. Rodrigues, F.A., Soares,J.D.R. and Silva, R.A.L. 2014. Anatomy of vegetative organs and seed histochemistry of Physalis peruviana L. Australian Journal of Crop Science, 8(6):895-


37. Silva, K.N. and Agra, M.F. 2005. Comparative pharmacobotanical study on Nicandra physalodes and Physalis angulata (Solanaceae). Revista Brasileira de Farmácia, 15: 344-351.


38. Thepsithar, C. and Thongpukdee, A. 2013. Comparative micro-morphology, anatomy and architecture of leaf of Physalis. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering. 7(8): 143-156.


39. Torki-Harchegani, M., Ghasemi Pirbalouti, A. and Ghanbarian, D. 2018. Influence of microwave power on drying kinetic, chemical composition and antioxidant capacity of peppermint leaves. Journal of Essential Oil Bearing Plants, 21: 430-439.


40. Tsialtas, J.T., Kostoglou, E., Lazari, D. and Eleftherohorinos, I.G. 2018. Annual Datura accessions as source of alkaloids, oil and protein under Mediterranean conditions. Industrial Crops and Products, 121: 187-194.


41. Ziegler, J. and Facchini, P.J. 2008. Alkaloid biosynthesis: metabolism and trafficking. Annual Review of Plant Biology, 59: 735-69.