The effect of different solvents on total phenolic, flavonoid contents and antioxidant activity of different organs of Momordica charantia L. cultured in Sistan region

Subject Areas : Medicinal Plants

forough Firoozkouhi ¹ , ُSedegheh Esmaeilzadeh Bahabadi ² , zeynab mohkami ³ , foroogh yousefzaei ⁴

1 - Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
2 - Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
3 - Institute of Biotechnology Agriculture, University of Zabol, Zabol, Iran
4 - 3Department of Biology, Faculty of science, Urmia University, Urmia, Iran

Received: 2017-08-05 Accepted : 2018-01-16 Published : 2018-02-20

Keywords: Antioxidant, Momordica charantia, Phenolicand Flavonoid compounds, Solvent,

Abstract :

Antioxidants metabolites which are protect the body against damage caused by free radical activity. Phenolic compounds are secondary metabolites in medicinal plants, which have a high antioxidant power. Karela (Momordica charantia L.) is an herb belonging to Cucurbitaceae family, consisted of phenolic compounds which can act as antioxidant. The present study was conducted to investigate the effect of different solvents (methanol, acetone, ethyl acetate, hexane and chloroform) on total phenolic, flavonoids content and antioxidant activities of Karela in Sistan region in 2016. Different extracts of plant parts ( Root, leaves and fruit) were obtained by different solvents by maceration method. Total phenolic and flavonoid contents were measured by Folin–Ciocalteu and Aluminum chloride methods, respectively. Antioxidant activity of extracts were determined by using Diphenyl Picryl hydrazyl (DPPH), Hydrogen peroxide (H2O2) and Ferric Reducing Antioxidant Power (FRAP) assays. The results were showed that the highest phenolic content was observed in fruit and leaf methanol extract (36.96±1.33 and 33.01±3.03 mg gallic acid/g DW respectively) and the least amount of phenol was extracted by ethyl acetate, hexane and chloroform which was less than 1 mg/g DW. The highest flavonoid content was observed in leaf acetone and methanol extract (10.95±1/97 and 10.03±2.2 mg quercitin/g DW respectively). Fruit methanol extract showed higher antioxidant activity than other extracts in three methods. Based on results of this research, Karela fruit can be suggested as useful source of natural antioxidants

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