Optimization of inulin extraction from Cynara scolymus L. root using response surface methodology (RSM)

Subject Areas : Medicinal Plants

Mohammad  Mohsenzadeh ¹ (2Associate Professor, Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad,)
Jalal  Ehsani ² (Ph.D Student of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran)
Morteza  Khomeiri ³ (3Associate Professor, Department of Food Science and Technology, Gorgan University of Agriculture and Natural Resources, Gorgan, Iran)
azem  Ghasem Nejad ⁴ (4Assistant Professor, Department of Medical Plants, Gorgan University of Agriculture and Natural Resources, Gorgan, Iran)

Received: 2016-02-25 Accepted : 2016-07-27 Published : 2016-09-01

Keywords: optimization, Artichoke (Cynara scolymus L.), Inulin, Response surface methodology  ,

Abstract :

Inulin is a low digestable or indigestible fiber which is found in various plants. Inulin amount ranging from 1% in Banana to more than 15% in Chicory roots changing. It has a positive impact on the composition of the intestinal flora, mineral absorption, blood lipid composition and prevents colon cancer. Artichoke (Cynara scolymus L.) is one of the most important sources of inulin. The aim of the present study was extraction of inulin from artichoke root and optimization of extraction conditions to achieve the highest extraction efficiency. For this purpose, the artichoke roots were collected from the research stead of Gorgan University of Agricultural Sciences and Natural Resources in september 2015 and inulin extraction with using of warm water solvent and response surface methodology (RSM)and central composite design (CCD) was performed. Response surface methodology in comparison to voluminous methods such as full factorial is preferred. In this technique for optimization of inulin extraction process, three variables extraction time (35-65 min), extraction temperature (55-95 °C) and the ratio of water to the roots (3:1 to 9:1)as independent variables were considered and their effect on the extraction efficiency of inulin were examined. After performing experiments, linear, 2FI and quadratic models on the inulin extraction yield were evaluated, also lack of fit test, coefficient of determination (R2)and Adjusted coefficient of determination (R2adjusted) for investigation the adequacy of the model were calculated. Study of the lack of fit test as well as R2 and  ​​R2adjusted values were showed the suitability of quadratic model. This model was used to find the best condition of extraction process with maximum efficiency. Results showed that the optimum condition for extraction of inulin with highest extraction yield (8.01%) related to temperature (95 °C), time (35.67 min) and ratio of water to root (9:1) with desirability of (92%).  

References:

1. Abbasi, S. and Farzan Mehr, H. 2009. Optimization of inulin extraction from Cirsium vulgare with and without ultrasound using response surface methodology. Journal of Science and Technology of Agriculture and Natural Resources, 47: 423-435. (In Persian)


2. Askari, S.A., Attar Movahedian, Gh., A., Naderi, A., Amini, F. 2008. The effect of artichoke (Guandelia tournefortil) on some biochemical factors contributing to atherosclerosis in an animal model. Quarterly Herb, 28(4): 112-119.


3. Azza, A.A., Hala, A.T., Ferial, M.A. 2011. Physico-chemical properties of inulin produced from jerusalem artichoke tubers on bench and pilot plant scale. Australian Journal of Basic and Applied Sciences, 5: 1297-1309.


4. Baldini, M., Danuso, F., Turi, M., Vannozzi, G. P. 2004. Evaluation of new clones of Jerusalem artichoke (Helianthus tuberosus L.) for inulin and sugar yield from stalks and tubers. Industrial Crops and Products, 19: 25-40.


5. Barkhatova, T. V., Nazarenko, M.N., Kozhukhova, M.A., Khripko, I. A. 2015. Obtaining and identification of inulin from jerusalem artichoke tubers. Foods and Raw Materials. 3(2): 13-22.


6. Berghofer, E., Cramer, A., Schmidt, V., Veigl, M. 1993. Pilot-scale production of inulin from chicory roots and its use in foodstuffs. Inulin and inulin containing crops. Studies in Plant Science, 3: 77-84.


7. Bloordi, M. 1999. Production of kambojia using plant extract containing inulin. MS.c Thesis. Faculty of agriculture biosystem. University of Tehran. (In Persian).


8. Darajani, P., Hosseini Nezhad, M., Shorideh, H., Abdollahian-Noghabi, M., Kadkhodaee, R., Balandari, A., Milani, E. 2015. Comparison of fructan yield of foreign cultivars and indigenous landrace of chicory and optimizing its extraction by response surface method (RSM). Journal of Research and Innovation in Food Science and Technology, 4(4): 343-354. (In Persian).


9. Dominguez, A. L., Rodrigues, L. R., Lima, N.M., Teixeira, J. A. 2014. An overview of the recent developments on fructooligosaccharide production and applications. Food Bioprocess Technol, 7: 324–337.


10. Franck, A. 2002. Technological functionality of inulin and oligofructose. British journal of Nutrition, 87: 287-292.


11. Gibson, G.R. 2004. Fibre and effects on probiotics (the prebiotic concept). Clinical Nutrition Supplements, 1: 25-31.


12. Grieve, M. 1984. A modern herbal, the medicinal, culinary, cosmetic and cconomic  properties, cultivation and folklore of herbs, grasses, fungi, shrubs and trees with all their modern scientific uses. Savvas Publishing, Suffolk.


13. Hellwege, E. M., RAAP, M., Gritscher, D., Willmitzer, L., Heyer, A.G. 1998. Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1-FFT cDNAs. Federation of European Biochemical Societies (FEBS)  Letters, 427: 25-28.


14. Hellwege, E. M., Czapla, S., Jahnke, A., Willmitzer, L., Heyer, A.G. 2000. Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus L.) roots. Proceedings of the National Academy of Sciences, 97: 86-99.


15. Hoseini nejad, M., Nahardani, M., Elhami rad, A.H. 2012. Characterization of inulin extract from iranian native chicory in comparison with some other sources. Journal of Research and Innovation in Food Science and Technology, 1(1): 39-46. (In Persian)


16. Jamishdzadeh, A., Fereidooni, F., Salehi, Z., Niknahad, H. 2005. Hepatoprotective activity of Gundelia tourenfortii. Journal of Ethnopharmacology, 101(1): 233-237.


17. Kip, P., Meyer, D., Jellema, R. 2006. Inulins improve sensoric and textural properties of low-fat yoghurts. International Dairy Journal, 16: 1098-1103.


18. Kaur, N., Gupta, A. 2002. Applications of inulin and oligofructose in health and nutrition. Journal of Biosciences, 27: 703-714.


19. Ku, Y., Jansen, O., Oles, C.J., Lazar, E.Z., Rader, J.I. 2003. Precipitation of inulins and oligoglucoses by ethanol and other solvents. Food Chemistry, 81: 125-132.


20. Lattanzio, V., Kroon, P.A., Linsalata, V., Cardinali, A. 2009. Globe artichoke: A functional food and source of nutraceutical ingredients. Journal of Functional Foods, 1: 131-144.


21. Laurenzo, K.S., Navia, J.L. and Neiditch, D.S. 2004. Preparation of inulin products. EP Patent 0: 787-745.


22. Li, J.W., Ding, S.D., Ding, X.L. 2007. Optimization of the ultrasonically assisted extraction of polysaccharides from Zizyphus jujuba cv. jinsixiaozao. Journal of Food Engineering, 80: 176-183.


23. Li, W., Zhang, J., Yu, C., Li, Q., Dong, F., Wang, G., Gu, G., Guo, Z. 2015. Extraction, degree of polymerization determination and prebiotic effect evaluation of inulin from Jerusalem artichoke. Carbohydrate Polymers, 121: 315–319.


24. Lingyun, W., Jianhua, W., Xiaodong, Z., Yalin, F. 2007. Studies on the extracting technical conditions of inulin from Jerusalem artichoke tubers. Journal of Food Engineering, 79: 1087–1093.


25. Lopez-Molina, D., Navarro-Martínez, M. D., Rojas-Melgarejo, F., Hiner, A. N. P., Chazarra, S., Rodríguez-López, J.N. 2005. Molecular properties and prebiotic effect of inulin obtained from artichoke (Cynara scolymus L.). Phytochemistry, 66: 1476-1484.


26. Milani, E., Koocheki, A., Golimovahhed, Q. A. 2011. Extraction of inulin from Burdock root (Arctium lappa) using high intensity ultrasound. International Journal of Food Science and Technology, 46: 1699-1704.


27. Milani, A., Poor Azarang, H., Vatankhah, Sh., Vakilian, H. 2010. Optimization of inulin extraction from Helianthus tubers using response surface methodology. Iranian Journal of Food Science and Technology Research, 6(3): 176-183. (In Persian)


28. Milani, A., Goli movahhed, Gh., Hoseini, F. 2011. Application of response surface methodology for optimization of inulin extraction from Salsify Plant. Journal of Food Research, 21 (1): 35-43. (In Persian)


29. Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31: 420-428.


30. Mishela, T., Nadezhda, P., Panteley, D., Albert., K. 2015. Characterization of inulin from Helianthus tuberosus L. obtained by different extraction methods – Comparative study. Scientific works of university of food technologies. 62: 461-464.


31. Montgomery, D.C. 2008. Design and Analysis of Experiments, 7th edn. John Wiley and Sons, Inc., Hoboken, NJ.


32. Nahardani, M., Hoseini Nejad, M., Elhami Rad, A.H. 2012. Evaluation of perebiotic effects and quality properties of inulin extracted chicory. Journal of Innovation in Food Science and Technology. 4(4): 87-96. (In Persian)


33. Panchev, I., Delchev, N., Kovacheva, D., Slavov, A. 2011. Physicochemical characteristics of inulins obtained from Jerusalem artichoke (Helianthus tuberosus L.). Eur Food Res Technol, 233: 889–896.


34. Paseephol, T., Small, D., Sherkat, F. 2007. Process optimisation for fractionating Jerusalem artichoke fructans with ethanol using response surface methodology. Food Chemistry, 104: 73-80.


35. Pourfarzad, A., Habibi Najafi, M.B., Haddad Khodaparast, M. H., Hassanzadeh Khayyat, M. 2015. Optimization of aqueous extraction of fructan from tubers of Eremurus spectabilis using box-behnken design. Iranian Food Science and Technology Research Journal. 11(5): 535-545. (In Persian)


36. Pourfarzad, A., Habibi Najafi, M.B., Haddad Khodaparast, M.H., Hassanzadeh Khayyat,  M. 2014. Characterization of fructan extracted from Eremurus spectabilis tubers: a comparative study on different technical conditions. Journal of Food Science and Technology, 33: 10-20.


37. Pourfarzad, A., Haddad Khodaparast, M.H., Habibi Najafi, M.B., Hassanzadeh Khayyat,  M. 2013. Efficacy of ultrasound in the extraction of fructan from tubers of Eremurus spectabilis using Box-Behnken design. Journal of Research and Innovation in Food Science and Technology, 2(3): 219-228. (In Persian)


38. Rezzoug, S.A., Maache-Rezzoug, Z., Sannier, F., Allaf, K. 2008. A Thermomechanical Preprocessing for Pectin Isolation from Orange Peel with Optimisation by Response Surface Methodology. International Journal of Food Engineering, 4, [online].


39. Saengthongpinit, W., Sajjaanantakul, T. 2005. Influence of harvest time and storage temperature on characteristics of inulin from Jerusalem artichoke (Helianthus tuberosus L.) tubers. Postharvest Biology and Technology, 37: 93-100.


40. Samavati, V. 2013. Polysaccharide extraction from Abelmoschus: Optimization by response surface methodology. Journal of Carbohydrate Polymers, 95: 588-597.


41. Sepulveda, E., Saenz, C., Aliaga, E., Aceituno, C. 2007. Extraction and characterization of mucilage in Opuntia spp. Journal of Arid Environments, 68: 534-545.


42. Shweta, T., Kulathooran, R., Laxmi, M., Lingamallu, J. 2015. Microwave-assisted extraction of inulin from chicory roots using response surface methodology. Journal of Nutrition and Food Sciences, 5(1): 1-7.


43. Tungland, B., Meyer, D. 2002. Nondigestible oligo‐and polysaccharides (dietary fiber): their physiology and role in human health and food. Comprehensive Reviews in Food Science and Food Safety, 1: 90-109.


44. Van Loo, J., Cummings, J., Delzenne, N., Englyst, H., Franck, A., Hopkins, M., Kok, N., Macfarlane, G., Newton, D., Quigley, M. 1999. Functional food properties of non-digestible oligosaccharides: a consensus report from the ENDO project (DGXII AIRII-CT-94-1095). British journal of Nutrition, 81: 121-132.


45. Vlaseva, R., Ivanova, N., Petkova, M., Todorova, M., Denev, P. 2014. Аnalysis of fermented lactic acid dairy products enriched with inulin-type fructans. Scientific Bulletin. Series F. Biotechnologies, 18: 145-149.


46. Zargari, A. 2000. Medical Plants. Tehran university publications, 3: 27-200.