Ecophysiological acclimation and salinity amelioration of soil Cyanobacterium Anabaena sp. FS 76 collected from oil polluted regions under ccombined effects of salinity and extremely limited irradiances

Subject Areas : Genetic

Shadman Shokravi ¹ , Elahe Kiaei ² , Afsane Pakzad ³ , Hamide Sadat Amirlatifi ⁴

1 - Department of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran
2 - Young Researchers and Elite Club, Islamic Azad University, Gorgan Branch, Gorgan, Iran
3 - Department of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran
4 - Young Researchers and Elite Club, Islamic Azad University, Gorgan Branch, Gorgan, Iran

Received: 2015-08-30 Accepted : 2016-04-24 Published : 2016-05-21

Keywords: Cyanobacteria, Ecophysiology, Anabaena sp.FS 76, Petroleum, Salinity amelioration,

Abstract :

The issue of salinity stress caused by oil extraction has been one of the major environmental issues of the country in the last decade. Biological agents in oil-contaminated areas can be considered the most efficient methods. The aim of this study was to reduce salinity by cyanobacteria isolated from oil contaminated areas. Soil cyanobacterium Anabaena sp. FS 76 were collected from oil polluted areas of southern Iran (Khuzestan province) and were studied ecophysiologically for acclimation ability at combination of different salinities (up to 1%) and extremely limited irradiances (2 μmol quanta.m-2S-1) at laboratory conditions. Samples were collected from oil polluted soils at different stations of Khuzestan province (Khark) for one year. Anabaena sp.FS 76 showed common strain and was selected for next steps after preliminary identification. Salinity treatments included Salt-free medium and BG011 medium with 0.25, 0. 5, 0.75, and 1% sodium chloride which were applied before and after limited irradiance treatments. Analysis involved growth analysis, Pigments (phycocyanin, allophycocyanin, phycoerythrin) and protein and sugar content assays. Results showed that the maximum growth rate may be observed at 1% salinity. At this range of salinity. 1% NaCl not only may not decrease total sugar and protein content but also cause significant increase. In vivo absorption spectra support the presence of central and rod sections of phycobilisome. This strain has salinity amelioration ability at the laboratory conditions. This potentiality reaches to highest degrees of salinity especially at 1% probability level. The results showed examples of environmental physiology and biotechnology powerful is for future studies.

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References

Abbasi, B., Shokravi, Sh. and Soltani, N. (2012). Studying of viability, growth and pigment composition and ammonium of Anabaena sp. Fs76 in different organic and inorganic nitrogen medium. M.Sc Thesis On plant Physiology, Islamic Azad University of Gorgan.

Ahmadi Livani, H. (2011). Studying of survival and growth soil cyanobacterium to salt stress, carbon dioxide and pH. M.Sc Thesis On plant Physiology, Islamic Azad University of Gorgan.

Amirlatifi, F., Shokrav, Sh., Safaie, M. and Hosseini Kolbadi, Z. (2008).  Studying of survival and growth cyanobacterium Nostoc sp. in pH stress and limited carbon dioxide. Journal of Plant Science Research. 6:38-45.

Amirlatifi, H., Shokravi, Sh. and Soltani, N. (2012). Studying of acclimation of soil cyanobacterium  Anabaena sp. FS 76 to combination effect of pH and carbon dioxide at extremely limited irradiance. M.Sc Thesis On plant Physiology, Islamic Azad University of Gorgan.

Anagnostidis, K. and Komarek, J. (1990) Modern approaches to the classification of   cyanobacteria. Stigonematales. Archieves for Hydrobiology. 14:224-286.

Anand, N., Radha, L., Shanthakumar, R.,  Hopper, S., Revathi, G. and Subramanian, TD. (1990). Blue-green algae as biofertilizers: certain view points on the choice of suitable isolates. In Perspective in phycology, ed. Rajarao, V.N. pp. 383-391. New Delhi: Today and Tomorrow’s Printer and Publisher.

Apte, S.K. and Bhagwat, A.A. (1997). Salinity-stress-induced proteins in two nitrogen-fixing Anabeana strains differently tolerant to salt. Journal of  Bacteriology. 171:909-915.

Badeli, Z., Derakhshanpour, J. and Shokravi, Sh. (2012). Studying the effect of salinity and limited irradiance in survival and growth of soil cyanobacterium  Anabaena sp. FS 76 and Nostoc sp. FS 77. M.Sc Thesis On plant Physiology, Islamic Azad University of  Tonekabon .

Bermejo, R.N.R., Alva´rez-Pez, J.M., Acie´n Ferna´ndez, F.G. and Molina Grima, E. (2002). Recovery of pure B-phycoerythrin from the microalga Porphyridum Cruentum. Journal of Biotechnology. 93: 73-85.

Boussiba, S. (1988). Anabaena azollae as biofertilizer. In: Algal biotechnology, eds,  Stadler, T.,J., Millon, M.C.Verdus, Y.Karamanos,H.Morvan and D.Christiaen, Elsevier Applied Science.

Burns R., Danielle, J., MacDonald, C., Mc Ginn, J.P., and Campbell, D.A. (2005). Inorganic carbon repletion disrupts photosynthetic acclimation low temperature in the cyanobacteria Synechococcus elongatus. Journal Phycology.  41: 322-334.

Chakigar, Sh., Shokravi, Sh. and Sateii, A. (2012). Study aclimation of the soil cyanobacteria Microchaete sp.FS13 to salt stress and salinity ammelioration potentiality at the laboratory condition MS.c Thesis On plant Physiology, Islamic Azad University of Gorgan.

Desikachary, T. V. (1959). Cyanophyta. Indian council of agricultural research, monographs on Algae New Delhi, India.

Jafari, A. (2013). Study morphological variation patterns soil cyanobacteria in ph stress and study the effect of morphological fluidity as acidity and alkalinity adjustment strategy. MS.c Thesis On plant Physiology, Islamic Azad University of Gorgan.

John, D.M., Whitton, B.W., Brook, A.J. (2002) The Freshwater Algal Flora of The British Isles -Cambridge University Press.

Kaushik, B. D. (1987). Laboratory methods for blue-green algae. Associated Publishing Company.

Kiaei, E., Soltani, N., Mazaheri assadi, M., Khavari nejad, R.A. and Dezfulian, M.  (2014). Evaluation of the effect of environmental factors on cyanobacteria with ability of biodiesel production. Ph.D Thesis of Microbiology.

Lanzhou, C., Dunhai, L. and Yongding, L. (2003). Salt tolerance of Microcoleus vaginatus Gom., a cyanobacterium isolated from desert algal crust, was enhanced by exogenous carbohydrates. Journal of Arid Environments. 55(4): 645–656.

Pakzad, A., Masoudian, N., Shokravi, Sh., Amirlatifi, H. and Abbasi, B. (2011). Analysis of morphological and ultrastructure variation patterns in two previously unexplored strains of nostocaceae collected from paddy fields of Gorgan (Golestan Province). Environmental Sciences the First National Conference of Phycology of Iran. 9: 73-83.

Safaei, M., Shokravi, Sh., Amirlatifi, F. and Hoseinii kolbadi, Z. (2012). cyanobacterium Fischerella sp. in Different salinities. Quarterly Journal of Plant Science Researches. 4:72-78.

Safaie Katoli, M., Nejad–Sattari, T., Majd, A. and Shokravi, Sh. (2015). Physiological, morphological and ultrastructural responses of cyanobacterium Fischerella sp. FS 18 to combination effects of extreme conditions. Journal Apply Environment Biology Science. 5(1):135-149.

Sasani, Z. (2010). Acclimation possibility of micro algae as a biological shield in agriculture to carbon dioxide extreme situations caused by possible attacks. MS.c Thesis On plant Physiology, Islamic Azad University of Gorgan.

Shokravi, Sh. and Soltani, N. (2011). Acclimation of the Hapalosiphon sp. FS 56 (cyanoprokaryota) to combination effects of dissolved inorganic carbon and pH at extremely limited irradiance. International journal on Algae. 13 (4): 379-391.

Shokravi, Sh., Amirlatifi, F., Safaie, M., Ghasemi, Y. and Soltani, N. (2006). Some physiological responses of Nostoc sp. JAH 109 to the combination effects of limited irradiance, pH and DIC availability. Quarterly Journal on Plant Science Researches. 1(3):55-63.

Shokravi, Sh., Safaie, M. and Jorjani, S. (2010). Studying of acclimation of the cyanobacterium  Haplosiphon sp. FS 44 to the combination Effects of pH and carbon dioxide concentration. Quarterly Journal on Plant Science Researches. 5(3): 31-42.

Shokravi, Sh., Soltani, N. and Baftehchi, L. (2009). Cyanobacteriology. Islamic Azad University. First express.

Soltani N., Khavarinejad, R.A., TabatabaeiYazdi, M. and Shokravi, Sh. (2007). Growth and metabolic Feature of cyanobacteria Fischerella sp. FS18 in different Combined nitrogen sources. Iranian Journal of Science. 18(2): 123-128.

Soltani N., Zarrini G., Ghasemi, Y., Shokravi, Sh. and Baftechi, L. (2008). Characterization of soil cyanobacterium Fischerella sp. FS18 under NaCl stress Journal of Biological Sciences.7(6): 931-936.

Soltani, N., Khavari-Nejad, R., Tabatabaie, M., Shokravi, Sh. and Fernandez Valiente, E.F. (2006). Variation of Nitrogenase Activity, photosynthesis and pigmentation of cyanobacteria Fischerella ambigua strain FS18 under different irradiance and pH. World Journal Microbiology Biotechnology. 22 (6): 571-577.

Soltani, N., Baftechi, L., Dezfulian, M., Shokravi, Sh. and Alnajar, N. (2012). Molecular and Morphological Characterization of Oil Polluted Microalgae. International Journal of Environmental Research. 6(2): 481-492.

Soltani, N., Khavari-Nejad, R.A., Tabatabaei Yazdi, M., Shokravi, Sh. and Fernández-Valiente, E. (2005). Physiological and antimicrobial characterizations of some cyanobacteria in extreme environments. Ph.D Thesis On plant Physiology.

Soltani, N., Siahbalaie, R. and Shokravi, Sh. (2011). Taxonomical characterization of Fischerella sp. FS18- A multidisciplinary approach International journal on Algae. 1(9): 48-55.

Stal, L. (1995). Physiological ecology of cyanobacteria in  microbial  mats  and  other communities. -New Phytology. 131: 1-32.

Vakili, F., Ghorchibeigi, K., Soltani, N. and Shokravi, Sh. (2007). The effect of continuous illumination and photoperiods on growth and heterocyst frequency of cyanobacterium Fischerella ambigua from Golestan province. Quarterly Journal of Plant Science Researches. 1(2):11-20.