Production of haploid plants in melon (Cucumis melo L.) through induced parthenogenesis with gamma-irradiated pollen
Subject Areas : Tissue cultureAhmad Mokari 1 , Alireza Motallebi Azar 2 , Jaber Panahandeh 3
1 - University of Tabriz
2 - Department of Horticulture, Faculty Agriculture, University of Tabriz, Tabriz, Iran.
3 - University of Tabriz
Keywords: Cucumis melo.L, Gamma radiation, Parthenocarpy, Embryo rescue, Callus, Regeneration, haploid,
Abstract :
Production of double haploid plants is one of the most important tools to obtain superior parents in order to produce F1 hybrid seeds. Irradiated pollen technique is one of the most successful methods to produce haploid plants in vegetables. In this study, to produce haploid plants in Hani Dew x Samsuri hybrid melon, emasculated flowers were pollenated with gamma irradiated pollens. Pollen grains were irradiated with 100, 200, 300 and 400 Gray of gamma rays. Then the emasculated flowers were pollinated with these irradiated pollens on three different dates (July, August and September). Fruits were harvested 3 to 4 weeks after pollination. Immature embryos were rescued and cultured on E20A medium. Regeneration and shooting occurred after callus production. The results indicated that irradiation dose at 100 gray was the best dose for pollen viability, pollination success and fruit set. Also, June was the best date for these properties. The highest percentage of regeneration was observed at 200 gray Chromosome counting confirmed haploid plants occurrence. Gamma dose at 200 gray and June were the best dose and date to produce haploid plants. These results indicated that irradiation at lower doses could produce haploid plants in a short time and accelerate the process of oblation to F1 hybrid varieties.
