The Effect of Eight Weeks of Interval Training with Grape Seed Nanoparticles Supplementation on Bax and Bcl2 Gene Expression in the Heart Tissue of Myocardial Infarction Rats

Subject Areas : Open Access

Hamid Mohammadi Hosseinabadi ¹ , Khosro Jalali Dehkordi ² , gholamreza sharifi ³ , Zohreh Mazaheri Tirani ⁴

1 - Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
3 - Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
4 - Basic Medical Science Research Center, Histogenotech Company, Tehran, Iran

Received: 2021-08-15 Accepted : 2021-09-03 Published : 2021-05-22

Keywords: Aerobic training, Myocardial Infarction, BAX, BCL2, Grape Seed,

Abstract :

Introduction: The use of exercise training and herbal supplements to treat diseases and metabolic disorders has become common among people. Considering the health-improving effects of aerobic training and grape seed nanoparticles extract, the present study was performed to investigate the effect of eight weeks of aerobic training with grape seed extract supplementation on Bax and Bcl2 gene expression in the heart tissue of rats with myocardial infarction.Materials and methods: In this experimental study, 20 rats were divided into four groups of five animals, including: 1) control, 2) aerobic training, 3) grape extract, and 4) training + grape extract. To investigate the effects of myocardial infarction on the study variables, five healthy rats were placed in the healthy control group. For eight weeks, groups 3 and 4 consumed 150 mg of grape extract daily by gavage. Also, groups 2 and 4 performed aerobic interval training, with 7 interval alternations, each including 4 minutes with an intensity of 80-90% VO2max and 3 minutes with an intensity of 65-75% VO2max. The Kolmogorov- Smirnov and one-way ANOVA with Tukey’s post- hoc tests were used to analyze the findings (P≥0.05).Findings: Induction of myocardial infarction had a significant effect on increasing Bax gene expression and decreasing Bcl2 gene expression in heart tissue (P≥0.001). However, aerobic training along with grape seed nanoparticle extract consumption had a significant effect on reducing Bax gene expression and increasing Bcl2 gene expression in the heart tissue of rats with myocardial infarction (P≥0.01).Conclusion: It seems that aerobic training along with grape extract consumption has more favorable effects on improving Bax and Bcl2 gene levels in myocardial infarction than either alone.

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