The effect of dietary copper supplementation in on the concentration of copper in equine serum, hoof and hair
Subject Areas :
Veterinary Clinical Pathology
GH.A Moghadam
1
,
علی Hassanpour
2
,
جعفر Rahmani
3
,
یوسف Davoodi
4
1 - Professor, Department of Animal Sciences, University of Tabriz, Tabriz, Iran
2 - Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 - Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4 - Assistant Professor, Faculty of Veterinary Medicine, Sarab Branch, Islamic Azad University, Sarab, Iran
Received: 2012-08-23
Accepted : 2012-11-21
Published : 2012-11-21
Keywords:
Copper,
horse,
Hair,
Serum,
Hoof,
Abstract :
This study was conducted to evaluate the effect of dietary copper supplementation on the concentration of this element in equine serum, hair and hoof. Forty stallions were divided in two groups of control and treatment each consisting of 20 horses. Copper sulfate was supplied in the diet of the treatment group for 2 months. Blood samples were taken from jugular vein of all horses on days 0, 15, 30, 45 and 60 and the sera were separated. Samples from hair and hoof were also collected. The concentration of copper in these samples was analyzed by atomic absorption spectrometry. In the control group, mean serumic concentration of copper diet not show any significant changes from day 0 to 60 but in the treatment group there were significant differences between different sampling days (p= 0.000). Mean copper concentration on the hair of the control group did not show a significant difference between different sampling days but in the treatment group a non significant increase was observed (p= 0.056). There was no significant difference in mean copper concentration of hoof in both treatment groups (p= 0.055, p= 0.481). There was a significant correlation between mean concentration of copper in the serum and hair and hoof at most sampling times. In conclusion, dietary supplementation of copper for 2 months leads to its significant serumic increase and non significant increase in hair and hoof of horses and considering the protective effects of copper on skin and also hoof, its dietary supplementation is recommended in sports horses.
References:
Andrieu, S. (2008). Is there a role for organic trace element supplements in transition cow health? Vet J, 176:77-83.
Anderson, B.C. (1990). Copper deficiency & posterior paralysis (shalel) in ruminants in the sultamate of Oman. Animal research center agriculture small Canada, Ottawa.
Arthington, J. (1996). Copper antagonists in Cattle Nutrition, Range cattle research and education center, on a department of animal sciences university of Florida - IFAS, 31:277 -281
Art,T. and Lekeux, P. (2005). Exercise-induced physiological adjustments to stressful conditions in sports horses. Livest Prod Sci, 92:101-111.
Basiland, M.R. (1993). Hypocuperosis: A clinical investigation of dairy in north land. NZJ. Vet med, 21:252-58
Bender, G.T. (1987). Principles of chemical instrumentation W. B sander co , pp : 107-118
Bejma, J.J. (1999). Aging and acute exercise enhance free radical generation in rat skeletal muscle. J Appl Physiol 87:465-470.
Chiaradia, E., Avellini, L., Rueca, F., Spaterna, A., Porciello, F. and Antonioni, M.T. (1998). Physical exercise, oxidative stress and muscle damage in racehorses. Comp Biochem Physiol B-Biochem Mol Biol, 119:833-836
Crocher, A. (1992). Effect of copper deficiency on resistance to trypanosome levvisi J-Nah Med Assoc, 84(8): 697-706.
Demorffarts, B., Kirschvink, N., Art, T., Pincemail, J. and Lekeux, P. (2005). Effect of oral antioxidant supplementation on blood antioxidant status in trained thoroughbred horses. Vet J, 169: 65-74
Donald, E. (1998). Copper deficiency in food animals, education article, 15:8-9.
Fur man, N.H. (1990). Standard Methods of Chemical Analysis. 6th Edition, Saunders, London, pp. 374-381.
Gart R.J. (2004). Copper deficiency in sheep and cattle. Deprtment of agriculture farm note, (28):94-98.
Hartlova, H., Rajmon, R., Dorflerova, A., Zita, L., Rehak, D., Rosmus, J. Sindelar, M. and Klabanova, P. (2008). Effect of dietary supplementation with vitamin E and selenium in Thoroughbred horses on the concentration of F2-isoprostanes in the blood plasma as a marker of lipid peroxidation. ACTA VET. BRNO, 77:335–340.
Howell, J.M. and Gow thome , J.M. (1986). Copper on animal and man. 2nd Edition, CRC Press, volum (I, II), pp. 32-48.
Kinnunen, S., Hyypa, S., Lappalainen, J., Oksala, N., Venojarvi, M., Nakao, C., Hannienen, O., Sen, C.K. and Atalay, M. (2005). Exercise-induced oxidative stress and muscle stress protein responses in trotters. Eur J Appl Physiol, 93: 496-501.
Montuschi, P., Barnes, P.J. and Roberts, L.J. (2004). Isoprostanes: markers and mediators of oxidative stress. FASEB J, 18:1791-1800.
NRC. (1989). Nutrient requirements of horses. 5th rev. Edition. National Academy Press, Washington, D.C., pp. 101.
Radostitis, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D. (2007). Veterinary Medicine, a text book of the disease of cattle, sheep, pigs and horses. 10th Edition, Elsevier Publishing, pp. 1661-1668.
Reckelhoff, J.F., Kanji, V., Racusen, L.C., Schmidt, A.M., Yan, S.D., Morrow, J., Roberts, L.J. and Salahudeen, A.K. (1998). Vitamin E ameliorates enhanced renal lipid peroxidation and accumulation of F-2-isoprostanes in aging kidneys. Am J Physiol-Regul Integr Comp Physiol, 274:R767-R774.