Study on sedative effects of different fractions of Hop (Humulus lupulus L.) extract compared with diazepam in rats

Subject Areas : Veterinary Clinical Pathology

رامین Shishehgar ¹ , علی Rezaie ² , ایلیاد Iesa beiglou ³ , محمد Jalilzadeh Hedayati ⁴ , CH Ahmadizadeh ⁵ , سلما Asl Faeghi ⁶ , A.R Ebadi ⁷

1 - Department of Basic Science, Ahar Branch, Islamic Azad University, Ahar, Iran
2 - Department of Clinical Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 - Department of Pharmacology, Tabriz Medical University, Pharmacy Faculty, Tabriz, Iran
4 - Department of Chemistry, Ahar Branch, Islamic Azad University, Ahar, Iran
5 - Department of Microbiology, Tabriz Branch, Islamic azad University, medical faculty, Tabriz, Iran
6 - Graduate of Agricultural Engineering, Tabriz Branch, Islamic Azad University, Tabriz, Iran
7 - Department of Microbiology, Tabriz Branch, Islamic azad University, medical faculty, Tabriz, Iran

Received: 2012-05-09 Accepted : 2012-09-05 Published : 2012-05-21

Keywords: Rat, Humulus lupulus, Polar fraction, Semi-polar fraction, Non-polar fraction, Dimethylsulfoxide,

Abstract :

Humulus lupulus is a medicinal plant which in Farsi is called "razak". The purpose of this research is, studying the sedative effects of polar, semi polar and non polar fractions extracted from Hop (Humulus lupulus. L) in comparison with diazepam in the animal model of Rat. For conducting this research polar, semi polar and non polar fractions extracted from Hop based on the polarity of solvent. Then study continued with the injection of obtained extracts and other medicines to different groups of Wistar breed of rats. First group was injected with 100mg/kg of Polar fraction extract the second group, with 100mg/kg of Semipolar extract, the third group, with 100 mg/kg of non-polar extract of Humulus lupulus the fourth group with 2 mg/kg of Diazepam the fifth group with the same volume of DMSO used as solvent of injectable medicines an the sixth group was the control group and did not receive any drug. The method of injection was Intra peritoneal (IP) form. Statistical diagrams and results showed a significant decreasing of anesthetic induction time and increasing of sleeping time of Ketamin induced anesthesia, after IP injection of the Polar fraction extract of Humulus lupulus. The results obtained showed that the polar-fraction extract of Humulus lupulus has more sufficient sedative effects than diazepam and other under studied groups.

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