Evaluation of the effect of adding different levels of n-3 fatty acids to the Bioxcell® extender on freezing ability of goat semen

Subject Areas : Veterinary Clinical Pathology

مهدی  Ansari ¹ (Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran)
آرمین  Towhidi ² (Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran)
محمد  Moradi Shahrbabak ³ (Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran)

Received: 2011-03-19 Accepted : 2011-06-08 Published : 2011-02-20

Keywords: Semen, n-3 fatty acid, Freezing ability, Bioxcell®,

Abstract :

The objective of this study was to investigate adding different levels of a n-3 fatty acid source to the Bioxcell® extender on freezing ability of goat semen. Six mature Mahabadi bucks were selected and semen was collected using an artificial vagina. Semen samples were pooled together and divided into four equal groups, after evaluating for qualitative characteristics. Treatment groups were supplemented by 0, 0.1, 1 and10 ng/ml levels of n-3 fatty acid, respectively. The rates of motility, progressive motility, viability and sperm abnormality were evaluated and recovery rate was calculated. Data were analyzed using proc GLM of SAS. Percentage of motility and recovery rate were significantly higher in 10 ng/ml group than the other groups.  Percentage of progressive motility was significantly higher in 10 and 0.1ng/ml than the other groups. Percentage of viability was significantly lower in 1ng/ml group than the other groups. Percentage of abnormality was not significant between the levels of n-3 fatty acid. The results indicated that adding of 10 ng/ml n-3 fatty acids to extender could improve freezing ability of goat semen.

References:

1. خوشوقت، ع. 1387. اثر تغذیه منبع اسیدهای چرب 3-n بر خصوصیات کمی و کیفی و ترکیب اسیدهای چرب اسپرم و میل جنسی در گاو هلشتاین. پایان نامه کارشناسی ارشد فیزیولوژی دام. گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران.


2. نصیری، ا. ح. 1387. اثر افزودن منبع اسیدهای چرب 3 n- و ویتامین E به رقیق کننده بر قابلیت انجماد اسپرم گاو هلشتاین. پایان نامه کارشناسی ارشد فیزیولوژی دام. گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران.


3. Amorim, E.A.M, Graham, J., Spizziri, B., Meyers, M., Amorim, L.S. and Torres, C.A.A. 2008. The effect of Adding Cholestrol, Vitamin A, Cod liver or Flax Oil Loaded Cyclodextrin on Bull Sperm Cryosurvival. 16th Congress on Animal Reproduction, Hungary, p: 29.


4. Badr, M.R., Abdel-Malak, M.G. and Shaker, M. H. 2004. Influence of some fatty acids and cholesterol addition to semen extender on freezability and in vitro fertilizing potential of ram spermatozoa. Assiut Vet. Med. J. 50: 304-318.


5. Castellini, S., Lattaioli, P., DalBosco, A., Minelli, A. and Mugnai. C. 2002. Oxidative status and semen characteristics of rabbit buck as affected by dietary vitamin E, C and n-3 fatty acid. Reprod. Nutr. Develop. 43: 91–103.


6. Connor, W.E., Lin, D.S., Wolf, D.P. and Alexander, M. 1998. Uneven distribution of desmosterol and docosahexaenoic acid in the heads and tails of monkey sperm. Journal of Lipid Research, 39: 1404-141.


7. Hafez, B. and Hafez, E.S.E. 2000. Reproduction of farm animals. P123-158, seventh edition. Lippincott Williams and Wilkins, Phyladelphia.


8. Kelso, K.A., Cerolini, S., Noble, R.C., Sparks, N.H.C. and Speake. B.K. 1996. Lipid and antioxidant changes in semen of broiler fowl from 25 to 60 weeks of age. Reprod. Fert. 106: 201-206.


9. Maldjian A., Pizzi, F., Gliozzi, T., Cerolini, S., Penny, P. and Noble, R. (2005): Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology, 63: 411-421.


10. Perez-Pe, R., Cebrian-Perez, J.A. and Muino-Blanco, T. 2001. Semen plasma proteins prevent cold shock membrane damage to ram spermatozoa. Theriogenology, 56: 425-434.


11. Purdy P.H. 2006. A review on goat sperm cryopreservation. Small Ruminant Res. 63: 215–225.