Investigation on the hygienic quality of natural mineral waters sold in the city of Tabriz in 2008

Subject Areas : Veterinary Clinical Pathology

جلیل Khandaghi ¹ , M.R Sehat khah ²

1 - Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Sarab Branch, Sarab, Iran
2 - Food and Drug Administration, Tabriz University of Medical Science, Tabriz, Iran

Received: 2009-04-08 Accepted : 2010-01-04 Published : 2009-08-23

Keywords: Coliform, NMW (Natural Mineral Water), ACC (Aerobic Coloni Count), Membrane filtration, MPN (Most Probable Number),

Abstract :

With the significant increase in bottled natural mineral water production and consumption over the last decade in our country, there has been a growing concern over the microbiological quality of such products. The purpose of the present study was to analyze various bottled natural mineral waters sold in Tabriz. In this study, 150 samples of such products were examined for aerobic colony count, coliforms, fecal coliforms, Escherichia coli, fecal streptococci, Clostridium perfringenes and Pseudomonas aeruginosa. Aerobic colony count pseudomonas and clostridium were determined using membrane filtration method and most probable number (MPN) method was used for determining coliforms and fecal streptococci. The results indicated that in 25.3% of the samples there was no microbial growth in plate count agar media and 2% of the samples were contaminated with high levels of aerobic colony count (more than 20 bacteria/ ml). Furthermore, of the 72.6% of samples with acceptable aerobic colony count, 2.75%, 1.83%, 2.75% and 0.91% were contaminated with coliforms, fecal streptococci, pseudomonas and clostridium respectively.

References:

1. رضویلر، و. (1378): میکروب­های بیماری­زا در مواد غذایی و اپیدمیولوژی مسمومیت­های غذایی، چاپ اول، انتشارات دانشگاه تهران، صفحات: 95 – 84.


2. مؤسسه استاندارد و تحقیقات صنعتی ایران (1365): ویژگی­های باکتریایی آب معدنی، انتشارات مؤسسه استاندارد و تحقیقات صنعتی ایران، شماره 2591.


3. مؤسسه استاندارد و تحقیقات صنعتی ایران (1382): آب معدنی، روش آزمون میکروبیولوژیکی، انتشارات مؤسسه استاندارد و تحقیقات صنعتی ایران، شماره 5869.

1. 4.  Cowman, S. and Kelsey, R. Bottled Water. In: Vanderzant, C. and Splittstoesser, D.F. (1992):  Compendium of methods for the microbiological examination of foods. 3^rd ed., APHA , WashingtonDC, pp: 1031-1036.

5. Harrigan,W.F. Microbiological examination of specific foods-Water. In: Harrigan,W.F. (1998): Laboratory methods in food microbiology. 3^rd ed., Academic press, London, pp: 298-305.


6. Hunter, R.P. (1993): The microbiology of bottled natural mineral waters (a review). Journal of applied bacteriology, (74): 345-352.


7. Kerr, M., Fitsgerald, M., Sheridan, J.J., Mcdowell, D.A. and Blair, I.S. (1999): Survival of Escherichia coli O157:H7 in bottled natural mineral water. Journal of applied microbiology, (87): 833-841.


8. Loy, A., Beisker, W. and meier, H. (2005): Diversity of bacteria growing in natural mineral water after bottling, Applied and environmental microbiology, (71): 3624-3632.


9. Mac Fadin, J.F. Section ш: Identification schems-gram negative bacteria. In: Mac Fadin, J.F. (2000): biochemical tests for identification of medical bacteria, 3^rd ed., Lippincott, Williams&Wilkins, Washington, USA, pp: 705-731.


10. Mavridou, A. (1992): Study of the microbial flora of a non-carbonated natural mineral water. Journal of applied bacteriology, (73): 355-361.


11. Tamaguini, L.M. and Gonzales, R.D. (1997): Bacteriological srability and growth kinetics of Pseudomonas aeroginosa in bottled water. Journal of applied microbiology, (83): 91-94.


12. Warburton, D., Harrison, B., Crawford, C., Foster, R., Fox, C., Gour, L. and Krol, P. (1998): A further review for the microbiological guality of bottled water soled in Canada: 1992-1997 survey results. International journal of food microbiology, (39): 221-226.