Study on the effect of Savory)Saturejakhuzestanica(essential oil and Polysorb toxin-binder against experimental aflatoxicosis in Japanese quail

Subject Areas : Veterinary Clinical Pathology

homa  arak ¹ (دانشجوی کارشناسی ارشد پرورش و تولید طیور، دانشگاه تربیت مدرس، تهران، ایران.)
mohammadamir  karimitorshizi ² (استادیار گروه پرورش و تولید طیور، دانشگاه تربیت مدرس، تهران، ایران.)
shaban  rahimi ³ (استاد گروه پرورش و تولید طیور، دانشگاه تربیت مدرس، تهران، ایران)

Received: 2013-12-08 Accepted : 2014-03-03 Published : 2013-11-22

Keywords: liver, Aflatoxin, pathology, <i>Saturejakhuzestanica<i>, Polysorb,

Abstract :

Anti-aflatoxicosis potential of dietary supplementation of savory essential oil and a commercial mycotoxin binder (Polysorb) was investigated by study of some internal organ weights, lipid oxidation of meat and liver lesions. A total of 144 male 21 days old Japanese quails were divided into six treatments and 4 replicates (6 birds in each replication)and treated for 10 wks as follows: A-Negative control, B-Positive control-fed diet contaminated with aflatoxin B1 (2.5 PPM), C- Contaminated feed+300 PPM savory essential oil,D-Contaminated feed+ 600 PPM savory essential oil, E- Contaminated feed+ 900 PPM savory essential oil, F- Contaminated feed+ 2.5 g/kg Polysorb. At the end of the fourth and tenth weeks of experiment, two birds from each cage were randomly euthanized and relative weights of liver, spleen and bursa offabricius were determined and tissue samples of the liver were taken. The lipid oxidation in fresh and refrigerator meat samples were determined at the end of experiment. Use of 600 PPM of savory essential oil reduced the effects of aflatoxin on weights of bursa of fabricius and spleen. Highly intense fatty changes, large and small foci of hepatocellular degeneration and portal fibrosis caused by aflatoxin were observed in non-treated aflatoxicosis induced birds (group B). It seems that dietary supplementation of 600 PPM of savory essential oil and Polysorb can effectively reduce the toxic effects of aflatoxin B1 in Japanese quails.

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