Histopathology of gallbladder lesions of confiscated livers from cattle slaughtered in urmia abattoir
Subject Areas :
Veterinary Clinical Pathology
amir amniattalab
1
,
araz manaffar
2
1 - استادیار گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.
2 - دانشآموخته دکترای دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.
Received: 2015-09-21
Accepted : 2015-12-21
Published : 2015-11-22
Keywords:
cattle,
Histopathology,
Gallbladder,
Urmia slaughter-house,
Abstract :
During one month carcasses of 323 cattle (213 bulls and 110 cow) were inspected by referring to Urmia slaughter-house. Livers and gallbladders of 47 cattle had macroscopic changes. Histopathologic sections were prepared from injured tissues and for bacteriology, contents of injured and normal gallbladders were cultured. Major macroscopic changes that were observed in damaged livers and gallbladders were: increasing of gallbladder thickness, petechiae, choleliths in gallbladder, hepatolithiasis, hepatic fascioliasis and presence of dicrocoelium in gall bladder. Histological sections were stained by hematoxylin and eosin, periodic acid schiff and masson’s trichrome staining methods. Cholecystitis, hyperplasia of seromucosal glands’ congestion, hemorrhage, fat necrosis and increased thickness of gallbladder layers were observed. Also Escherichia coli was isolated from 4 gallbladders. In females, the incidence of microscopic changes including cholecystitis, increasing of mucosal layer thickness, hyperplasia of seromucosal glands, hemorrhage in layers except the mucosal layer and presence of bacteria in culture of gallbladder contents was more than males (p<0.05). Also incidence of cholecystitis in native breeds was more in comparison with hybrid and Holstein breeds (p<0.05). According to the results, prevalence of hepatic fascioliasis is a predisposing factor for cholecystitis in this area. Also traditional feeding and diet regimen of native cattle are effective in creation of cholelithiasis which arise as a result of cholecystitis.
References:
خاکی، ز. (1384). سنگ کیسه صفرا در گوسفندان و گاوهای ارجاعی به کشتارگاههای اطراف تهران. مجله دانشکده دامپزشکی دانشگاه تهران، دوره 60، شماره 4، صفحات: 403-401.
Agnew, D.W., Hagey, L. and Shoshani, J. (2005). Cholelithiasis in a wild African elephant (Loxodonta africana). Journal of Zoo and Wildlife Medicine, 36(4): 677-683.
Baer, J.F., Weller, R.E., Dagle, G.E., Malaga, C.A. and Lee, S.P. (1990). Cholelithiasis in owl monkeys: seven cases. Journal of the American Association for Laboratory Animal Science, 40(6): 629-633.
Cable, C.S., Rebhun, W.C. and Fortier, L.A. (1997). Cholelithiasis and cholecystitis in a dairy cow. Journal of the American Veterinary Medical Association, 211(7): 899-900.
Cheema, A.H. and Hooshmand-Rad, P. (1985). Experimental cholecystitis in goats caused by mature Fasciola gigantica. Research in Veterinary Science, 38(3): 292-295.
Cullen, J.M. (2007). Liver, biliary system and exocrine pancreas. In: Pathologic Basis of Veterinary Disease. McGavin, M.D. and Zachary, J.F. Editors. 4th ed., USA: Philadelphia, PA, Mosby Inc., pp: 453-454.
Degerli, S. and Ozcelik, S. (2003). The first giardia infection in cattle gallbladder. Turkish Journal of Veterinary and Animal Sciences, 27: 1231-1233.
Dias, F.S, Carrijo, K.F., Santos, I.F., Franco, R.M. and Nascimento, E.R. (2012). Characterization of bacterial microbiota present in bile and gallbladder epithelium of cattle. Veterinaria Noticias, 18(1): 20-28.
Holt, D.E., Mehler, S., Mayhew, P.D. and Hendrick, M.J. (2004). Canine gallbladder infarction: 12 cases (1993-2003). Veterinary Pathology, 41(4): 416-418.
Jones, T.C., Hunt, R.D. and King, N.W. (1997). Veterinary Pathology. 6th ed., USA: Baltimore, Lippincott Williams & Wilkins, pp: 1104-1106.
Kawai, K., Litagawa, H., Higashimori, T., Matusi, T., Fujiyama, S., Monna, T., et al. (1980). Experimental chronic non-sappurative destructive cholangitis in rabbits following immunization with bile duct antigen. Gastroenterology, 15(4): 337-345.
Petruzzi, J., Mangini, C. and Messa, C. (1988). Spontaneous cholelithiasis in sheep: prevalence and analysis of gallstones and biles. Journal of Comparative Pathology, 98(3): 367-369.
Raoofi, A., Rahmani Shahraki, A., Namjoo, A. and Momtaz, H. (2012a). Prevalence and pathological findings of cholelithiasis in goats. Iranian Journal of Veterinary Medicine, 6(2): 73-77.
Raoofi, A., Shahraki, A.R., Namjoo, A. and Momtaz, H. (2012b). A survey on prevalence and pathological findings of gallstones in Lori-Bakhtiari sheep in Iran. The Scientific World Journal, doi: 10.1100/2012/524607.
Rulli, F., Muzzi, M., D’Antini, P., Melissari, M., Sianesi, M. and Zanella, E. (1996). The histopathological and microbiological aspects in a model of acute acalculous cholecystitis: an experimental study. Acta Biomedica, 67(1-2): 61-67.
Hayes, M.A. (2007). Liver and biliary system. In: Pathology of Domestic Animals. Jubb, K.V.F., Kennedy, P.C. and Palmer, N.S. Editors. 5th ed., UK: London, Saunders, pp: 345-348.
Tabaran, F., Bolfa, P., Nagy, A., Taulescu, M., Gal, A., Boari, C. et al. (2013). Severe gallbladder hydrops (mucocel) following obstructive cholangioma (biliary adenoma) in a cattle. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture, 70(1): 172-173.
Valero, M.A., Santana, M., Morales, M., Hernandes, J.L. and Mas-Coma, S., (2003). Risk of gallstone disease in advanced chronic phase of fasioliasis: An experimental stady in rat model. Journal of Infectious Diseases, 188(5): 787-793.
Van Erpecum, K.J., Wang, D.Q., Moschetta, A., Ferri, D., Svelto, M., Portincasa, P., et al. (2006). Gallbladder histopathology during murine gallstone formation: relation to motility and concentrating function. Journal of Lipid Research, 47(1): 32-41.