Evaluation of acute phase proteins in cows affected by Traumatic Reticuloperitonitis

Subject Areas : Veterinary Clinical Pathology

Ali  Rezaei ¹ (Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran)
A.P  Rezaei Saber ² (Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran)
افشین  Davasaz Tabrizi ³ (Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran)

Received: 2008-10-29 Accepted : 2009-01-19 Published : 2008-11-21

Keywords: Protein, Cow, peritonitis, reticulum,

Abstract :

     Traumatic Reticuloperitonitis is one of the most important diseases of the bovine digestive system which if diagnosed in early stage and before severe adhesions develop, more effective treatments leading to better prognosis could be under taken. In the course of accurate and timely diagnosis                            of these diseases, acute phase proteins of the affected animals were evaluated. For this purpose,                       blood samples were taken form jugular vein of 25 Holstein cows in which the presence of disease         clinically and confirmed with laparatomy and their sera separated. Blood sample were also collected from 20 healthy cows as the control group. After preparing serum sample, total protein haptoglobin, serum amyloid A and ceruloplasmin were measured in both groups. Total protein was determined by the Biuret method, haptoglobin and serum amyloid A by ELISA and ceruloplasmin through determination of the oxidative activity. Statistical analysis indicated significant difference in the amounts of total protein, haptoglobin and serum amyloid A between the two groups (p<0.05) but there was no significant difference in the amount of ceruloplasmin. This study demonstrated that was of haptoglobin and serum amyloid A could be beneficial n the diagnosis of bovine traumatic reticuloperitonitis.                

References:

1. نادعلیان، م. (1374): بیماری‌های دستگاه گوارش نشخوارکنندگان، انتشارات دانشگاه تهران، صفحات: 196-184.


2. Alsemgeest, S.P.M., Kalsbeek, H.C., Wensing, T., Koeman, J.P., Van Ederen, A.M. and Gruys, E. (1994): Concentrations of serum amyloid-A and haptoglobin as parameters of inflammatory disease in cattle, Veterinary Quarterly, 16: 21-23.


3. Alsemgeest, S.P.M., Taverne, M.A.M., Boosman, R., Vanderweyden, B.C. and Gruys, E. (1993): Peripartum acute-phase protein serum amyloid-A concentration in plasma of cows and fetuses. American Journal of Veterinary Research, 54: 164-167.


4. Anderson, N.V. (1992): Veterinary Gastroenterology. 2nd ed., Lea & Febiger, USA, pp: 712-721, 725-734.


5. Bakes, J. and Illek, J. (2006): Plasma ceruloplasmin and fibrinogen during enzyme therapy of mastitis in dairy cows. Acta Veterinary Brno, 75: 241-240.


6. Conner, J.G. Eckersall, P.D., Wiseman, A., Bain, R.K. and Douglas, T.A. (1989): The acute phase response in calves following infection with Pasteurella haemolytica and Ostertagia ostertagii and endotoxin administration. Research in   Veterinary Science, 47: 203-207.


7. Eckersall, P.D. (2000): Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue de Medicine Veterinary, 151(7): 577-584.


8. Eckersall, P.D. (1995): Acute phase proteins as markers of inflammatory lesion. Comparative Hematology, 5: 1-5.


9. Gruys, E. and Toussaint M.J.M. (2005): Acute phase reaction and acute phase proteins. Journal of Zhejiang University Science, 68(11): 1045-1056.


10. Gruys, E., Obwolo, M.J. and Toussaint, M.J.M. (1994): Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry: a review. Veterinary Bulletin, 64: 1009-1018.


11. Hirvonen, J., Huszencza, G., Kulcsar, M. and Pyorala, S. (1999): Acute phase response in dairy cows with acute postpartum metritis, Theriogenology, 51: 1071-1083.


12. Hofner, M.C., Fosbery, M.W., Eckersall, P.D. and Donaldson, A.I. (1994): Haptoglobin response of cattle infected with foot-and-mouth disease virus. Research in Veterinary Science, 57:125-128.


13. Horadagoda, N.U., Knox, K.M.G., Gibbs, H.A., Reid, S.W.J., Horadagoda, A., Edwards, S.E.R. and Eckersall, P.D. (1999): Acute phase proteins in cattle: discrimination between acute and chronic inflammation. Veterinary Record, 144: 437-441.


14. Jafarzadeh, S.R., Nowrouzian, I., Khaki, Z., Ghamsari, S.M. and Adibhashemi, F. (2004): The sensitivities and specificities of total plasma protein and plasma fibrinogen for the diagnosis of traumatic reticuloperitonitis in cattle. Preventive Veterinary Medicine, 65: 1-7.


15. Johnson, R.W. (1997): Inhibition of growth by pro-inflammatory cytokines; an integrated view. Journal of Kerreman, 22: 175-178.


16. Karreman, H.J., Wentink, G.H. and Wensing T. (2000): Using serum amyloid-A to screen dairy cows for subclinical inflammation. Veterinary Quarterly, 22: 175-178.


17. Murata, H., Shimada, N. and Yoshika, M. (2004): Current research on acute phase proteins in veterinary diagnosis: an overview. The Veterinary Journal, 168: 28-40.


18. Petersen, H.H. (2004): Application of acute phase protein measurement in veterinary clinical chemistry. Veterinary Research, 35: 163-187.


19. Salonen, M., Hirvonen, J., Pyorala, S., Sankari, S. and Sandholm, M. (1996): Quantitative determination of bovine serum haptoglobin in experimentally induced Escherichia-coli mastitis. Research in Veterinary Science, 60:88-91.


20. Skinner, J.G., Brown, R.A.L. and Roberts, L. (1991): Bovine haptoglobin response in clinically defined field conditions. Veterinary Record, 128: 147-149


21. Sunderman, F.W. and Nomoto, S. (1970): Measurement of human serum ceruloplasmin by its p-phenylendiamine oxidase activity. Clinical Chemistry, 16: 903-910.