Evaluation of the antibacterial property of Garden Thyme (Thymus vulgaris L.) essential oil following the experimental exposure of broilers to Salmonella Typhimurium (ATCC: 14028) and its ability to prevent tissue complications in the small Int
Subject Areas : Veterinary Clinical PathologyYounes Anzabi 1 , یوسف Doustar 2 , Shayan Doolat-Abadi 3
1 - Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
2 - Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University- Tabriz Branch, Tabriz, Iran
3 - D.V.M. Graduate, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.
Keywords: : Garden Thyme, Broiler Chickens, Salmonella Typhimurium, Experimental infection, Tissue complications,
Abstract :
In order to apply the use of plant essential oils in complementary medicine, it is necessary to investigate their effects in inhibiting the growth of pathogenic bacteria in the laboratory, animal model and also pay attention to their possible side effects. Therefore, the purpose of the present study was to investigate the antibacterial effects of garden thyme essential oil on Salmonella Typhimurium and to evaluate its ability to prevent possible side effects caused by experimental exposure to the mentioned bacterium. For this purpose, 35 pieces of 14-day-old male broiler chickens were divided into 5 groups of 7 including healthy control, gavage control, and experimental patient, treated with garden thyme essential oil and treated with Oxytetracycline. First day 0.5 ml of Salmonella Typhimurium microbial suspension and the next day, separately 1 ml of garden thyme essential oil and Oxytetracycline to all the chickens of the third to fifth groups and also 1 ml of sterile distilled water was gavage to the chickens of the second group twice a day with a time interval of 12 hours for 7 days. At the end of each stage, the microbial count of the stool samples was performed and at the end of the period, after the easy killing of the chickens, the pathological examination of the small intestine and liver samples of all birds was performed. The result is that the chickens that were treated with oxytetracycline had a significant decrease in the excretion and colonization of Salmonella Typhimurium.
