Litholytic activity of hydro-alcoholic extract of Equisetum arvense L. in male rats

Subject Areas : animal physiology

maryam eidi ¹ , Alireza Sadeghipour ² , Ali Mazooji ³ , Reza Ghahramani ⁴

1 - biology department
2 - Pathology Department, Rasoule-Akram Hospital, Tehran, Iran
3 - Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
4 - Department of Biology, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran

Received: 2021-09-14 Accepted : 2021-09-16 Published : 2021-02-19

Keywords: Rat, Crystallization, Calcium oxalate, Kidney stone, Equisetum arvense,

Abstract :

In the present study, effect of Equisetum arvense aerial part hydro-alcoholic extracts on calcium oxalate crystallization was studied in male rats. At first, hydro-alcoholic extract of Equisetum arvense at doses of 25, 50 and 100 mg/kg were prepared. Crystallization of calcium oxalate was induced by adding ammonium chloride and ethylene glycol in drinking water for 3 and 38 days, respectively. Control group was treated by drinking water. Experimental groups were treated by extract at doses 25, 50 and 100 mg/kg for 38 days. After 38 days, animals were anesthetized by ether and left kidneys removed and put them in buffered formaldehyde. Specimens were prepared for histological studies and stained by H&E. Results showed that treatment of extract decreased number of crystals in kidney sections of experimental animals in comparison with control group. Also, administration of plant extract decreased tubule-interstitial damages in treated animals. Also, the extract decreased tubule-interstitial damages of tissue in treated animals. So, the treatment of plant could be confirmed by this research.