Evaluation of the Antimicrobial Effect of Lemon verbena leaves Extracts on Streptococcus pyogenes, Staphylococcus epidermidis, Klebsiella pneumoniae and Brucella melitensis in vitro and Animal Model Study

Subject Areas :

shahrzad nasiri semnani ¹ , nastaran ghassempoor ²

1 - Assistant Professor in Department of Microbiology, Factualty of Bacic Sciences -Engineering, Zanjan Branch, Islamic Azad university, zanjan.Iran.
2 - PhD. student General Veterianary, Factualty of Veterianary Razi University, Kermanshah. Iran.

Received: 2020-07-22 Accepted : 2020-09-20 Published : 2020-09-22

Keywords:

Abstract :

Inroduction & Objective:Streptococcus pyogenes and Staphylococcus epidermidis Klebsiella pnenmonia and Brucella melitensis have high resistance to antibiotics treatments. The aim of this study, was to investigate the antimicrobial effect of Lemon verbena leaves extracts on Streptococcus pyogenes, Staphylococcus epidermidis, Klebsiella pneumoniae and Brucella melitensis in vitro and animal model study. Material and Method:In this studies after prepagation, of extracts Lemon leaves MIC and MBC and  diameter of inhibition zone was determined by well diffusion agar method, the extracts on bacteria determined by broth dilution method. In animal model study, 5×105 CFU/ml bacteria were injected intraperitioneally in to mice and the counts of bacterias colonies was countected according to the standard protocol. Results: The results of macrodilution method showed that the MIC of ethanolic extract of the leaves of the lemon verbena in the range of 1:32 dilution (27.81 mg/ml) to 1:8 (111.25 mg/ml) and MBC ethanolic extract of the leaves of the lemon verbena  in the range 1:16 (55.62 mg/ml) to 1:4 (222.5 mg/ml). MIC of acetonic extract of the leaves of the lemon verbena in the range of 1:32 dilution (27.81 mg/ml) to 1:8 (111.25 mg/ml) and MBC of acetonic extract of the leaves of the lemon verbena  in the range 1:16 (48.18 mg/ml) to 1:4 (192.75 mg/ml). Also MIC of aqueous extract was in the range of 1:8 (103.75 mg/ml) to 1:4 (207.5 mg/ml) and MBC of this extract was in 1:4 dilutions (207.5 mg ml) in all studied bacteria. In animal model, were seen a significant reduction between the treated groups with control group. Ethanolic and acetonic extract Lemon verbena more effective in an animal model. Conclusion: The results of this study showed that Lemon verbena extracts has antimicrobial effect on studied bacteria

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