Effect of Viola tricolor hydro-ethanolic extract on serum glucose and lipids in streptozotocin-nicotinamide-induced diabetic rats

Subject Areas :

Abbas  Vakilian ¹ (Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Masoumeh  Asle Rousta ² (Islamic Azad University, Zanjan, Iran)
مهدی  رهنما ³ (Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.)

Received: 2023-08-30 Accepted : 2023-10-04 Published : 2023-10-23

Keywords: Lipid, Diabetes, Viola tricolor extract, Rat, glucose,

Abstract :

Background & Aims: Viola tricolor is one of the medicinal plants of the Violaceae family, which possesses antioxidant and anti-inflammatory properties. This study aims to investigate the effect of V. tricolor hydro-ethanolic extract on glucose levels and serum lipid profile in diabetic rats.Materials & Methods: The rats were injected intraperitoneally with streptozotocin and nicotine to induce diabetes. Diabetic rats were divided into four groups. The first group received no treatment, and the second to fourth groups received glibenclamide and extract of V. tricolor with doses of 300 and 600 mg/kg for 30 consecutive days by gavage. Blood glucose level, lipid profile, and body weight were assessed at the end of the period.Results: When administered at a dose of 600 mg/kg, V. tricolor extract significantly reduced the levels of glucose, cholesterol, triglycerides, and low-density lipoprotein in the serum of diabetic rats. It also increased high-density lipoprotein and body weight in these animals.Conclusion: The results indicate that the Viola tricolor is beneficial in reducing complications caused by type 2 diabetes.

References:

1.American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes care. 2010;33(Supplement_1):S62-9.

2.Wellen KE, Hotamisligil GS. Inflammation, stress, and diabetes. Journal of Clinical Investigation. 2005;115(5):1111-9.

3.Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circulation Research. 2010;107(9):1058-70.

4. Forouhi NG, Wareham NJ. Epidemiology of diabetes. Medicine. 2019;47(1):22-7.

5.Verma S, Gupta M, Popli H, Aggarwal G. Diabetes mellitus treatment using herbal drugs. International Journal of Phytomedicine. 2018;10(1):1-0.

6.Vukics V, Kery A, Guttman A. Analysis of polar antioxidants in heartsease (Viola tricolor L.) and garden pansy (Viola x wittrockiana Gams.). Journal of Chromatographic Science. 2008;46(9):823-7.

7.Vukics V, Kery A, Bonn GK, Guttman A. Major flavonoid components of heartsease (Viola tricolor L.) and their antioxidant activities. Analytical and Bioanalytical Chemistry. 2008;390:1917-25.

8.Toiu A, Pârvu AE, Oniga I, Tămaş M. Evaluation of anti-inflammatory activity of alcoholic extract from Viola tricolor. Revista Medico-Chirurgicala a Societatii de Medici si Naturalisti din Iasi. 2007;111(2):525-9.

9.Hellinger R, Koehbach J, Fedchuk H, Sauer B, Huber R, Gruber CW, Gründemann C. Immunosuppressive activity of an aqueous Viola tricolor herbal extract. Journal of Ethnopharmacology. 2014;151(1):299-306.

10.Koike A, Barreira JC, Barros L, Santos-Buelga C, Villavicencio AL, Ferreira IC. Edible flowers of Viola tricolor L. as a new functional food: Antioxidant activity, individual phenolics and effects of gamma and electron-beam irradiation. Food Chemistry. 2015;179:6-14.

11.Siddiqi HS, Mehmood MH, Rehman NU, Gilani AH. Studies on the antihypertensive and antidyslipidemic activities of Viola odorata leaves extract. Lipids in Health and Disease. 2012;11(1):1-2.

12.Mojani MS, Sarmadi VH, Vellasamy S, Sandrasaigaran P, Rahmat A, Peng LS, Ramasamy R. Evaluation of metabolic and immunological changes in streptozotocin-nicotinamide induced diabetic rats. Cellular immunology. 2014;289(1-2):145-9.

13.Asadi S, Moradi MN, Khyripour N, Goodarzi MT, Mahmoodi M. Resveratrol attenuates copper and zinc homeostasis and ameliorates oxidative stress in type 2 diabetic rats. Biological Trace Element Research. 2017;177:132-8.

14.Saqib F, Mujahid K, Aslam MA, Modhi A, Moga MA, Bobescu E, Marceanu L. Ex vivo and in vivo studies of Viola tricolor Linn. as potential cardio protective and hypotensive agent: Inhibition of voltage‐gated Ca++ ion channels. FASEB Journal. 2020;34(7):9102-19.

15. Shirwaikar A, Rajendran K, Barik R. Effect of aqueous bark extract of Garuga pinnata Roxb. in streptozotocin-nicotinamide induced type-II diabetes mellitus. Journal of Ethnopharmacology. 2006;107(2):285-90.


16. Adam SH, Giribabu N, Rao PV, Sayem AS, Arya A, Panichayupakaranant P, Korla PK, Salleh N. Rhinacanthin C ameliorates hyperglycaemia, hyperlipidemia and pancreatic destruction in streptozotocin–nicotinamide induced adult male diabetic rats. European Journal of Pharmacology. 2016;771:173-90.


17. Gazor R, Niknezhad F, Yousefbeyk F, Aghajany-Nasab M, Mohammadghasemi F. The Extract of Viola odorata Flowers Improves the Biochemical, Pancreas Histological, and Insulin Resistance Parameters in an Animal Model of Diabetes. Iranian Journalof Toxicology. 2022;16(1):43-50.


18. Mousavi SH, Naghizade B, Pourgonabadi S, Ghorbani A. Protective effect of Viola tricolor and Viola odorata extracts on serum/glucose deprivation-induced neurotoxicity: role of reactive oxygen species. AvicennaJournal of Phytomedicine. 2016;6(4):434.


19. Mohammadi K, Mohammadi R, Asle-Rousta M, Rahnema M, Mahmazi S. Viola tricolor Hydroalcoholic Extract Improves Behavioral Deficiencies in Rats Exposed to Chronic Immobilization Stress. BrazilianArchives of Biologyand Technology. 2022;65: e22210267.


20. Hassanvand A, Saadatmand S, Lari Yazdi H, Iranbakhsh AR. Investigation of Antioxidant, Antimicrobial and Anticancer

 

Potential of Silver Nanoparticles Synthesized by Viola tricolor L. Extract. Journal of Agricultural Science and Technology. 2022;24(4):885-900.  

21. Novytska I, Gorbatovska N, Nikolaeva G, Tretyakova O, Gushcha S. The effect of the gel with tricolor violet extract on the activity of the antioxidant defense system in rats with a model of pаro-dontitis. Balneo and PRM Research Journal. 2023; 14 (2).


22. Nishikawa T, Araki E. Impact of mitochondrial ROS production in the pathogenesis of diabetes mellitus and its complications. Antioxidants& Redox Signaling. 2007;9(3):343-53.

23.Ganeshpurkar A, Saluja AK. The pharmacological potential of rutin. Saudi Pharmaceutical Journal. 2017;25(2):149-64.

24.Furusho T, Kataoka E, Yasuhara T, Wada M, Innami S. Administration of beta-carotene suppresses lipid peroxidation in tissues and improves the glucose tolerance ability of streptozotocin-induced diabetic rats. International Journal for Vitamin and Nutrition Research. 2002;72(2):71-6.

25. Marcelino G, Machate DJ, Freitas KD, Hiane PA, Maldonade IR, Pott A, Asato MA, Candido CJ, Guimarães RD. β-Carotene: preventive role for type 2 diabetes mellitus and obesity: a review. Molecules. 2020;25(24):5803.