The Effects of Different Levels of Dietary Galactooligosaccharide on Antioxidant Defence in Zebra Fish (Danio rerio)
Subject Areas :سمیرا یوسفی 1 , Seyed Hosein Hoseinifar 2 , عبدالمجید حاجی مرادلو 3 , حامد پاک نژاد 4
1 - گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان. ایران
2 - گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان. ایران
3 - گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان. ایران
4 - گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان. ایران
Keywords: Prebiotic, Antioxidant defence, Zebra fish, Galactooligosaccharide,
Abstract :
v:* {behavior:url(#default#VML);} o:* {behavior:url(#default#VML);} w:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);} >1024x768Normal0falsefalsefalseEN-USX-NONEAR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman","serif"; mso-bidi-font-family:"B Nazanin";} >Inroduction & Objective: The aim of the study was to study the effects of different levels of prebiotic galactooligosaccharide (GOS) on antioxidantdefense system in zebrafish (Danio rerio) as model organism. Material and Methods:A total number of 420 fish (45±0.1 mg) were supplied and randomly stocked in 60 L aquariums assigned to four treatments repeated in triplicates. Fish were with four experimental diets contain0, 0.5, 1 and 2% prebiotic for 8 weeks. At the end of feeding trial, total antioxidant activity as well as expression of antioxidant defense related genes Catalase (CAT) and Superoxide dismutase (SOD).Results:The obtained results revealed that administration of different levels of GOS had no significant effects on total antioxidant activity (P>0.05). Also, gene expression studies showed that CAT expression was remarkably down-regulated in fish fed 0.5 and 2% GOS (P0.05). Evaluation of SOD gene expression revealed significant down regulation in prebiotic fed fish (0.5, 1 and 2%) compared control group (P0.05).Counclusion:Based on these results, it seems that administration of GOS in zebra fish diet down-regulates antioxidant defense related genes without any effect on total antioxidant activity.
