EffectsOf FormalinandCopper Sulfate onGillTissuesOf KhazaWhite Fish(Rutiluskutum
Subject Areas :Masoud Farrokh roz 1 , Abass Ali Zamini 2 , Elahe Mozafari 3
1 - دانشگاه آزاد اسلامی واحد لاهیجان، استادیار گروه آبزیان و شیلات، لاهیجان.ایران.
2 - دانشگاه آزاد اسلامی واحد لاهیجان، استادیار گروه آبزیان و شیلات، لاهیجان.ایران.
3 - دانشگاه آزاد اسلامی واحد لاهیجان، کارشناسی ارشد گروه آبزیان و شیلات، لاهیجان.ایران.
Keywords: Copper, Rutilus Kutum, Histopathologic Changes, Formalin, Gill,
Abstract :
IntroductionandObjective:Formalinand copper sulfate are compoundsthanthosewho areconstantly inaquacultureto treatparasitic infectionsandbacterialinfectionsareused. Fishgillsare as organsthatareconstantlyexposed tothe external environmentandbecause ofits roleinrespirationandosmoticbalance ofthese chemicalsandheavy metalare thefirst target. The purpose ofthisstudyis ofhistopathologicaleffectsof copper sulfateandformaldehydeconcentrationsongilltissue ofwhite fishin the Caspian SeaMaterials and Methods: In this research, indication of probable was teforgill tissues of Caspianwhitefish with average weight of 428 mg and length weight of 3.5cm is addressed after exposing to different compactness of copper sulfate (in 20 minutes short time care and 24 hours long time care, 100ppm and 1ppm) and formalin (inshort time care and long time care, 25ppm and 250ppm). After stabilizingtissuesin10 percent formalin and making 5 micrometersection, it is painted with hematoxylin method and is studied by optical microscope.Results: The results showed that lying gill in the proximity of 1ppm per copper sulfate litter can cause to start as lighthy per plagy at gill secondary blade. In compactness of100ppm, these was tesare enhanced and brings about phenol mena such as hyperplagyin the gill primary and secondary lamell as and very much hyperemia was evident.Conclusion: The results of lyinggill in proximityof 25ppm formalin ,a little hyperemi a was evident and in long time cause the presence of red globule.
