Effects Of Formalin and Copper Sulfate on Skin Tissues Of Khazar White Fish (Rutilus kutum
Subject Areas :
Massoud
Farokhroz
1
(دانشگاه آزاد اسلامی واحد لاهیجان،لاهیجان.ایران)
Abbas Ali
Zamini
2
(دانشگاه آزاد اسلامی واحد لاهیجان،لاهیجان.ایران)
Elahe
Mozafari
3
(دانشگاه آزاد اسلامی واحد لاهیجان،لاهیجان.ایران)
Keywords: Formalin, Copper, white fish, Skin, Rutilus Kutum,
Abstract :
Inroduction and Objective: Formalinand copper sulfate are compoundsthanthosewho areconstantly inaquacultureto treatparasitic infectionsandbacterialinfectionsareused. Fishskinare as organsthatareconstantlyexposed tothe external environmentandbecause ofits roleinrespirationandosmoticbalance ofthese chemicalsandheavy metalare thefirst target. The purpose ofthisstudyis ofhistopathologicaleffectsof copper sulfateandformaldehydeconcentrationsonskintissue ofwhite fishin the Caspian SeaMaterial and Methods: In this research, indication of probable waste for skin tissues of Khazar white fish with average weight of 428 Mg and length weight of 3.5 Cm is addressed after exposing to different compactness of copper sulfate (in 20 minutes short time care and 24 hours long time care,100ppm and 1ppm) and formalin (in short time care and long time care,25ppm and 250ppm). After stabilizing tissues in 10 percent formalin and making 5 micrometer section, it is painted with hematoxylin method and is studied by optical microscope.Results:The results showed that in the consideration of skin histology, there was not any changes in skin cells which was in exposure of lowest compactness of copper, but in compactness of 100ppm disparting epidermis layers was more than basal layer. Keratinocyte layer existent in skin remained without any change and effects of bleeding was not seen.Conclusions:In considerations of skin, individuation of layers is seen in a mild extent and in long time care is seen in Malpigy cells and disparting epidermis layers from basal layers and effects of hyperemia has not been seen.
