Investigation of total phenolic and flavonoid contents, and evaluation of antioxidant activities of Verbascum cheiranthifolium Boiss. extract from Kolakchal region, Tehran Province

Subject Areas : phytochemistry

ZAHRA SADAT AGHAKHAH RAZLIGHI ¹ , Rustaiyan Abdolhossein ² , Kambiz Larijani ³

1 - Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2018-09-03 Accepted : 2018-12-06 Published : 2019-02-20

Keywords: DPPH, Phenolic compounds, Verbascum, Antioxidant, Flavonoids,

Abstract :

This plant Verbascum cheiranthifolium Boiss. belonging to the Scrophulariaceae family, is derived from traditional Iranian medicine and vicinal countries which is used both edible and topical for treatment. For the first time in this research the amount of total phenol, Flavonoid and antioxidant property of aerial parts extract of plant of Iran in the Kolakchal area (Tehran-Iran) were investigated. To measure The amount of Phenol and Flavonoid respectively is used from reagents Folin- Ciocalteu and Alcl3 and the method DPPH is used to measure the Antioxidant potential. The result showed the phenolic content for samples respectively 0.925 milligram of galic acid per gram dry weight and 18.887 milligram quercetin per gram dry weight. In evaluation of antioxidant potential by method DPPH amount of Ic50 for BHT 72301/428 µgr/ml and for the extract is 45829/364 µgr/ml. As a result, the antioxidant activity of this plant have higher potential against the antioxidant synthesis of BHT. The results of this study showed that this plant has a good antioxidant power against a variety of oxidative systems and as an accessible natural antioxidant can be used in food and pharmaceutical industries.