Chemical Composition of Rosemary and Dianthus Floral Waters by Dispersive Liquid Liquid Micro Extraction Followed by Identification of compounds using GC-MS
Subject Areas : MicrobiologyFereshte Nematollahi 1 , Kambiz Larijani 2 , Sirvan Mohammadi Azar 3
1 - استادیار دانشگاه آزاد اسلامی، واحد تهران شرق، گروه شیمی، تهران، ایران
2 - استادیار دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، گروه شیمی، تهران، ایران
3 - استادیار دانشگاه آزاد اسلامی، واحد سنندج، گروه شیمی، سنندج، ایران
Keywords: Dianthus, Floral Water, Liquid Liquid Micro Extraction, Rosemary,
Abstract :
Introduction: Customers prefer to use natural extracts instead of the synthetic products. The consumption of floral waters and extracts has become quite popular as a mean to overcome and relieve some slight problems and sicknesses. The economics, yields and purity of the extracts are important factors to consider. Suitable analytical extraction and statistical methods are required to help us for experimental design.Materials and Methods: Floral water of Rosemary and Dianthus were obtained from traditional companies. Analytical grade solvents were purchased from Merck chemical company of Germany. Different solvents namely ethanol, methanol, carbon tetrachloride and chloroform were employed for the extraction. The extracts were injected to GC- MS apparatus and the peaks were identified.Results: Statistical analysis indicated that with minimum volume of solvent mixtures of (442 QL of ethanol, 20 QL of carbon tetrachloride) extraction is accomplished. More than 88% of compounds in Rosemary and almost 100% of compounds in Dianthus floral waters are characterized.Conclusion: In this study, response surface method has been applied for optimization and it was shown that this method could be designed for the best micro extraction condition with the application of lowest volume of solvents.
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