Contamination of brucellosis in milk and cheese by Real time PCR
Subject Areas : microbiologyfatemeh Khodaverdipour 1 , Nazila Arbab Soleimani 2 , Yasaman Boroun 3
1 - Department of Microbiology, Faculty of Basic Siences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Department of Microbiology, Department of Microbiology,Damghan Branch, Islamic Azad University, Damghan,Iran
3 - Department of Microbiology, Faculty of Basic Siences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Keywords: Brucella, Brucellosis, milk, cheese, Real Time PCR,
Abstract :
Brucella spp are small, immobile, gram-negative bacteria that are lacking capsules and formed like cocobacillus. Brucellosis is a zoonosis infection between humans and animals that can lead to miscarriages, fertility disorders, genital infections, reduction in milk production, urethritis, and epididymitis in the original host, resulting in many medical disorders in patients and unnecessary treatment expenses. Moreover, farmers would end up facing significant economic losses. Despite advances in blood culture techniques and serological tests to detect specific antibiotics, there are still significant difficulties in the diagnosis of brucellosis; therefore, a new laboratory test is needed for a better examination. One of the most recent quantitative methods that have already caught the attention of many researchers is the detection of bacteria by Real Time PCR method. The aim of this study is to identify bacteria of the genus Brucella, both in milk and cheese samples, by the method of Real Time PCR. 25 samples of cow's milk and twenty-five samples of cheese were collected from different parts of Shahrekord city. DNA extraction was performed using the DNA extraction kit (Cinnagen company) for the molecular diagnosis. Then, by the use of Real Time PCR reaction (Corbett Rotor-Gene Model, manufactured in Australia), samples were studied. In this study, fifty samples were examined, and only two samples (4%) were diagnosed with Brucella abortus, meanwhile, there were no reports on infections by Brucella in the cheese samples. Conclusion: This study shows that molecular techniques such as Real Time PCR can be used as a complementary method for the detection of Brucella, alongside all the other common methods.
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