Isolation of phytase-producing fungi from the soil poultry house

Subject Areas : microbiology

Omid Nikbakht Chemazketi ¹ , Amir Hossain Esmaili ² , Issa Gholampour Azizi ³

1 - Faculty of Veterinary Medicine, Bob.C. Islamic Azad University, Babol, Iran.
2 - Department of Paramedicine, Bob.C. Islamic Azad University, Babol, Iran.
3 - Department of Mycology, Bab.C., Islamic Azad University, Babol, Iran

Received: 2025-06-12 Accepted : 2025-07-16 Published : 2025-09-26

Keywords: fungus, PSM, phytase, Aspergillus, soil,

Abstract :

Objective: Phosphorus is an essential micronutrient of plants and plays a role in important biological functions. In nature, phosphorus is mostly found in immobile minerals and in fixed organic forms, including phytic acid and phosphoesteric compounds. However, the bioavailability of limited phosphorus can be increased by using phosphate solubilizing microorganisms such as bacteria and fungi.

Materials and methods: Samples were taken from the depth of 3-5 cm of the soil where the poultry live and after preparing 10^-1 to 10^-5 dilutions and pore plate culture in Potato dextrose agar (PDA) medium containing antibiotics, after one-week storage at temperature 28 degrees, molds were isolated and identified. Then these molds were inoculated in PSM (Phytase Screening Medium) culture medium and the mushrooms that had the clearest aura were selected and the enzyme index (EI) and phosphate solubility efficiency (SE) were determined and finally the phytase enzyme activity was determined with a spectrophotometer was measured.

Findings: In this study, the fungi that were able to form a clear halo around the colony in the specific culture medium of phytase screening (PSM) included Aspergillus niger and Aspergillus flavus. Aspergillus flavus was more effective than Niger by 2.17%. Their average enzyme activity was 5.07±0.17 and 4.94±0.15 Iu/L, respectively.

Conclusion: This research can provide effective solutions for the development of an ideal phytase to improve animal nutrition, human health and environmental protection in the near future.

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