Investigating the prebiotic properties of yeast samples isolated from the wastewater of food industries

Subject Areas : Microbiology

M. Larypoor ¹ , M Golgharan ² , G Fotouhi ³ , A Akhavan sepahi ⁴ , R Samsami ⁵

1 -
2 - 'M.Se, Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran [e-mail]
3 - Ph.D.candidate in Microbiology,Faculty of Department of Biological Sciences Islamic Azad University Kish international Branch, Kish island, Iran Email: [e-mail]
4 - Master's student, Department of Biotechnology, Faculty of Chemical Engineering, Tarbist Modares University, Tehran, Iran Email: [e-mail]
5 - گروه شیمی، واحد دزفول، دانشگاه آزاد اسلامی، دزفول، ایران.

Received: 2025-03-08 Accepted : 2025-05-10 Published : 2025-03-05

Keywords: inulin, probiotic, prebiotic, polysaccharide, yeast,

Abstract :

Abstract

Introduction: The increasing prevalence of intestinal and stomach problems has increased attention to the use of dietary supplements and probiotics. The aim of this study was to evaluate the probiotic properties of polysaccharides obtained from yeasts isolated from food industry wastewater.

Materials and Methods: Sampling and purification of yeast was carried out from the wastewater of the food factory. The yeasts were identified by morphological, biochemical and microscopic methods. The prebiotic properties of yeast polysaccharide were compared with inulin. Structural analysis was performed using Infrared Fourier Transform and its resistance to acid and enzymatic digestion, effectiveness in the growth of probiotic bacteria, technological and antioxidant properties were evaluated and the best prebiotic producing strain was identified by molecular PCR method.

Results: Polysaccharides extracted from Saccharomyces cerevisiae, Candida kefyr, Geotrichum candidum showed resistance to digestion comparable and even better than inulin. The ability of oil absorption and water binding capacity for polysaccharides isolated from Saccharomyces cerevisiae (4.64±0.03, 1.81±0.03), Candida kefyr (4.58±0.03, 1.87±0.03), Geotrichum candidum (4.52±0.03, 1.92±0.03) were comparable with inulin and even higher than it. The antioxidant activity of Saccharomyces cerevisiae was higher than the two strains of Candida kefyr and Geotrichum candidum and about 40% in the studied concentrations. Also a direct relationship between polysaccharide concentration and increased antioxidant activity was observed.

Conclusion: The results indicated that the polysaccharides isolated from yeast have a better prebiotic ability than inulin. Therefore, yeast wall might be a good substitute for inulin with plant source and help to preserve plants.

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