Effect of Chronic Panax Ginseng Treatment on Histopathological Alterations of the hippocampal dentate gyrus in Pilocarpine-Induced Epilepsy Model Rats
Subject Areas : Experimental physiology and pathology
Mahin Ganjkhani
1
,
Reza Bigonah
2
1 -
2 - Zanjan University of Medical Sciences
Keywords: Keywords: Pilocarpine model epilepsy, Panax ginseng, Histopathology, Hippocampus,
Abstract :
Objective: Because mossy fiber sprouting is considered an important indicator in assessing the severity of temporal lobe epilepsy in animal models, the purpose of the present study is to investigate the protective effects of ginseng and sodium valproate on histopathologic changes in the dentate gyrus region of the hippocampus in pilocarpine-induced epileptic rats
Methods: In the present experimental study, 70 adult male Wistar rats were randomly divided into different groups. In 4 groups, lithium pilocarpine (127 mg/kg) was used as an intraperitoneal injection to model epilepsy. Then, three groups of epileptic groups were given ginseng 150 mg/kg, sodium valproate 200 mg/kg (as a positive control), and ginseng plus sodium valproate for thirty days, respectively. At the end of the treatment period and after hippocampal biopsy, Nissl staining was performed to evaluate cell number and Timms staining was performed to examine aberrant axonal sprouting. Data analysis was performed using SPSS software and ANOVA statistical tests.
Results: It was observed that ginseng treatment significantly (p<0.05) helped ameliorate the hippocampal damage compared to the epileptic non treatment group.
Conclusion: Ginseng treatment has a better effect than sodium valproate on improving and reducing complications of epilepsy in the hippocampus. It seems that ginseng prevents the destruction of neurons and the production of abnormal mossy fibers in epilepsy.
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