The protective role of probiotic mixture (Lactobacillus plantarum, Lactobacillus paracasei and Lactobacillus brevis) on the structure of small intestine tissue of mice infected with Staphylococcus aureus bacteria

Subject Areas : microbiology

Shiva saeidi ¹ , Zahra Keshtmand ²

1 - Ms Student, Departmant of Biology, Central Tehran Branch, Islamic Azad Univercity, Tehran, Iran
2 - Assistant Professor, Departmant of Biology, Central Tehran Branch, Islamic Azad Univercity, Tehran, Iran

Received: 2024-11-15 Accepted : 2025-01-29 Published : 2025-04-17

Keywords: probiotic, small intestine, Staphylococcus aureus, rat,

Abstract :

­Staphylococcus aureus is one of the most common causes of food poisoning,which causes food poisoning by containing numerous toxins. Probiotics are live and beneficial microorganisms that can modulate the host's immune response and prevent and treat various diseases..­The aim of this research is to investigate the protective effect of probiotic mixture(Lactobacillus plantarum,Lactobacillus paracasei and Lactobacillus brevis)on the small intestine tissue of male rats infected with Staphylococcus aureus bacteria.­In this experimental study­21 male Wistar rats were subjected to three the group included the control group,infected with Staphylococcus aureus­(10⁸ CFU/ml)and the infected model + probiotic recipient(10⁹CFU/ml).­Induction of infection was done by intraperitoneal injection and receiving probiotics for­35 days by gavage method. After the period of treatment and dissection of the animals, the tissue of the small intestine was extracted for examination in different groups.Data analysis in different groups was done with SPSS software and one-way statistical test of variance andP<0.05 was considered significant.Results:The results showed a significant change in the damage of different parts of the small intestine tissue in the infected group compared to the control group.­showed.While the positive changes in induced damage in the small intestine of the treatment group were significantly compared to the infected group.­The probiotic mixture showed a protective effect on the destruction of the small intestine tissue of rat infected with bacteria.­Therefore,­it can probably be used as a promising therapeutic tool to reduce small intestinal tissue damage

References:

1.Mitevska E, Wong B, Surewaard BG, Jenne CN. The prevalence, risk, and management of methicillin-resistant Staphylococcus aureus infection in diverse populations across Canada: a systematic review. Pathogens. 2021 Mar 10(4):393.
doi: 10.3390/pathogens10040393.
2.Liu A, Garrett S, Hong W, Zhang J. Staphylococcus aureus Infections and Human Intestinal Microbiota. Pathogens. 2024 March 13(4):276.
doi.org/10.3390/pathogens13040276.
3.Howden BP, Giulieri SG, Wong Fok Lung T, Baines SL, Sharkey LK, Lee JY, Hachani A, Monk IR, Stinear TP. Staphylococcus aureus host interactions and adaptation Nat. Rev. Microbiol. 2023 Jun 21(6):380-95.
doi: 10.1038/s41579-023-00852-y.
4.Hu X, Guo J, Zhao C, Jiang P, Maimai T, Yanyi L, Cao Y, Fu Y, Zhang N. The gut microbiota contributes to the development of Staphylococcus aureus-induced mastitis in mice. The ISME journal. 2020 Jul 14(7):1897-910.doi: 10.1038/s41396-020-0651-1.
5. Zhao C, Wu K, Hao H, Zhao Y, Bao L, Qiu M, He Y, He Z, Zhang N, Hu X, Fu Y. Gut microbiota-mediated secondary bile acid alleviates Staphylococcus aureus-induced mastitis through the TGR5-cAMP-PKA-NF-κB/NLRP3 pathways in mice. NPJ biofilms and microbiomes. 2023 Feb 9(1):8.
doi.org/10.1038/s41522-023-00374-8.
6. Chen H, Zhang J, He Y, Lv Z, Liang Z, Chen J, Li P, Liu J, Yang H, Tao A, Liu X. Exploring the role of Staphylococcus aureus in inflammatory diseases. Toxins. 2022 Jul 14(7):464.
doi: 10.3390/toxins14070464.
7.Piewngam P, Khongthong S, Roekngam N, Theapparat Y, Sunpaweravong S, Faroongsarng D, Otto M. Probiotic for pathogen-specific Staphylococcus aureus decolonisation in Thailand: a phase 2, double-blind, randomised, placebo-controlled trial. The Lancet Microbe. 2023 Feb 4(2):e75-83.
doi: 10.1016/S2666-5247(22)00322-6.
8.Tang J, Zhao M, Yang W, Chen H, Dong Y, He Q, Miao X, Zhang J. Effect of Composite Probiotics on Antioxidant Capacity, Gut Barrier Functions, and Fecal Microbiome of Weaned Piglets and Sows. Animals. 2024 Apr 14(9):1359. doi.org/10.3390/ani14091359.
9.Chandrasekaran P, Weiskirchen S, Weiskirchen R. Effects of Probiotics on Gut Microbiota: An Overview. Sci Rep . 2024 May 25(11):6022.
doi: 10.3390/ijms25116022.
10.Watanabe M, Nakai H, Ohara T, Kawasaki K, Murosaki S, Hirose Y. Beneficial effect of heat-killed Lactiplantibacillus plantarum L-137 on intestinal barrier function of rat small intestinal epithelial cells. Sci Rep .2024 May 14(1):12319.
doi.org/10.1038/s41598-024-62657-0.
11.Shawky LM, Abo El Wafa SM, Behery M, Bahr MH, Abu Alnasr MT, Morsi AA. Lactobacillus rhamnosus GG and Tannic Acid Synergistically Promote the Gut Barrier Integrity in a Rat Model of Experimental Diarrhea via Selective Immunomodulatory Cytokine Targeting.Molecular Mol Nutr Food Res. 2024 Aug 68(16):2400295.
doi: 10.1002/mnfr.202400295.
12.Di Vincenzo F, Del Gaudio A, Petito V, Lopetuso LR, Scaldaferri F. Gut microbiota, intestinal permeability, and systemic inflammation: a narrative review. Intern Emerg Med .2024Mar19(2):275-93.doi: 10.1007/s11739-023-03374-w.
13.Liang H, Wang Y, Liu F, Duan G, Long J, Jin Y, Chen S, Yang H. The Application of Rat Models in Staphylococcus aureus Infections. Pathogens. 2024 May 13(6):434.
doi.org/10.3390/pathogens13060434.
14.Ahmadi Z, Tajbakhsh H, Momtaz H. Detection of the antibiotic resistance pattern in staphylococcus aureus isolated from clinical samples obtained from patients hospitalized in imam raze hospital, kermanshah. World J Microbiol Biotechnol .2014 Mar 6(4 (17)):209-311.
doi.org/10.5812/jkums.118807.
15.Tabaei S, Kouhi Noghondar M, Mohammadzadeh, M,Ataei L, Amel Jamehdar S. Pattern of antibiotic resistance in methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from clinical specimens: Imam Reza hospital in Mashhad. Med. J. Mashhad Univ. Med. Sci.,2016 Jul-Oct 59(2): 64-70.
doi.org/10.5812/jkums.118807.
16. Liu Y, Zhang J, Ji Y. Environmental factors modulate biofilm formation by Staphylococcus aureus. Sci. Prog.. 2020 Mar103(1):0036850419898659.
doi: 10.1177/0036850419898659.
17. Barzin oshtologh A, Keshtmand, Z, Samadikhah H R. Effect of protective of a mixture of native Iranian probiotics (Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus holoticus) on the Damage of Rat male Small Intestinal tissue Caused by lead acetate. Iranian Journal of Biological Sciences. 2022 May 16 : 71-80.
18. Gulgun M, Erdem O, Oztas E, Kesik V, Balamtekin N, Vurucu S, Kul M, Kismet E, Koseoglu V. Proanthocyanidin prevents methotrexate-induced intestinal damage and oxidative stress. Exp Toxicol Pathol .2010 Mar 62(2):109-15.
doi: 10.1016/j.etp.2009.02.120.
19.Men Q, Zhang P, Zheng W, Song S, Ai C. Fucoidan alleviates Salmonella-induced inflammation and mortality by modulating gut microbiota and metabolites, protecting intestinal barrier, and inhibiting NF-κB pathway. Food Biosci.2023 Dec 1;56:103209.
doi.org/10.1016/j.fbio.2023.103209.
20. Rowe SE, Wagner NJ, Li L, Beam JE, Wilkinson AD, Radlinski LC, Zhang Q, Miao EA, Conlon BP. Reactive oxygen species induce antibiotic tolerance during systemic Staphylococcus aureus infection. Nat. Microbiol.. 2020 Feb 5(2):282-90.
doi: 10.1038/s41564-019-0627-y.
21.Siméon Pierre Chegaing Fodouop, Donatien Gatsing, Benjamin Talom Tangue, Richard Simo Tagne, Sédric Donald Tala, Joseph Tchoumboué, Jules Roger Kuiate,Effect of Salmonella typhimurium infection on rat's cell oxidation and in vivo antioxidant activity of Vitellaria paradoxa and Ludwigia abyssinica aqueous extract, Asian Pac J Trop Dis.2015 Jan 5(1): 38-46.
doi.org/10.1016/S2222-1808(14)60624-1.
22.Djenguemtar J, Goka MS, Noubom M, Konack EY, Kamsu GT, Sokoudjou JB, Feudjio HB, Kodjio N,Gatsing D. In vivo Antisalmonellal and Antioxidant Activity of Hydroethanolic Extract of Bauhinia rufescens Leaves in Wistar AlbinoRats Infected with Salmonella Typhi. Microbiol. Res. J. Int.2023Sep33 (2):39-53.
doi 10.9734/mrji/2023/v33i21366.
23.Hosseini S, Keshtmand Z, Mlrnurollahi S M. Investigating the effect of probiotics mixture (Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus heloticus) on small intestine tissue infected with Escherichia coli bacteria in male rats. J. Appl. Microbiol.2023Sep 2(9) 79-87.
doi.org/10.1186/s40104-021-00624-9.
24.Zhu T, Mao J, Zhong Y, Huang C, Deng Z, Cui Y, Liu J, Wang H. L. reuteri ZJ617 inhibits inflammatory and autophagy signaling pathways in gut-liver axis in piglet induced by lipopolysaccharide. JASB. 2021 Dec 12:1-6.
doi.org/10.1186/s40104-021-00624-9.
25. Teshfam M, Rahimi SH, Karimi K. Effect of various levels of probiotic ou morpliology of iutestinal mucosa in brioler chicks. J Vet Res. 2005 60(3):205-211.
26. Zhang J, Zhao J, Jin H, Lv R, Shi H, De G, Yang B, Sun Z, Zhang H. Probiotics maintain the intestinal microbiome homeostasis of the sailors during a long sea voyage.Gut Microbes.2020 Jul 11(4):930-43
.doi: 10.1080/19490976.2020.1722054.
27.Varela-Trinidad GU, Domínguez-Díaz C, Solórzano-Castanedo K, Íñiguez-Gutiérrez L, Hernández-Flores TD, Fafutis-Morris M. Probiotics: Protecting our health from the gut. Microorganisms. 2022 Jul 10(7):1428.
doi: 10.3390/microorganisms10071428.
28.Gorreja F, Walker WA. The potential role of adherence factors in probiotic function in the gastrointestinal tract of adults and pediatrics:a narrative review of experimental and human studies. Gut Microbes.2022 Dec 14(1):2149214. doi: 10.1080/19490976.2022.2149214.
29.Dvorožňáková E, Vargová M, Hurníková Z, Lauková A, Revajová V. Modulation of lymphocyte subpopulations in the small intestine of mice treated with probiotic bacterial strains and infected with Trichinella spiralis. J Appl Microbiol .2022 Jun 132(6):4430-9.
doi: 10.1111/jam.15534.
30.Cao F, Jin L, Gao Y, Ding Y, Wen H, Qian Z, Zhang C, Hong L, Yang H, Zhang J, Tong Z. Artificial-enzymes-armed Bifidobacterium longum probiotics for alleviating intestinal inflammation and microbiota dysbiosis. Nat. Nanotechnol. 2023 Jun18(6):617-27.
doi: 10.1038/s41565-023-01346-x.
31.Wu Y, Yang F, Jiang W, Hu A, Xiong Z, Yang S, Cao P, Cao Z, Xiong Z, Cao H. Effects of compound probiotics on intestinal barrier function and caecum microbiota composition of broilers. Avian Pathol. 2022 Sep 51(5):465-75.
doi.org/10.1080/03079457.2022.2100740.
32.Mazziotta C, Tognon M, Martini F, Torreggiani E, Rotondo JC. Probiotics mechanism of action on immune cells and beneficial effects on human health. Cells. 2023 Jan 12(1):184.
doi: 10.3390/cells12010184.
33. Qi X, Xiao Y, Zhang X, Zhu Z, Zhang H, Wei J, Zhao Z, Li J, Chen T. Probiotics suppress LL37 generated rosacea-like skin inflammation by modulating the TLR2/MyD88/NF-κB signaling pathway. FOOD FUNCT .l 2024;15(17):8916-34. doi.org/10.1039/D4FO03083D.
34. Winther KD, Boll EJ, Sandvang D, Williams AR. Probiotic Bacillus spp. enhance TLR3‐mediated TNF signalling in macrophages. J Immunol ; 2024 Mar 171(3):402-12. doi: 10.1111/imm.13721.