Comparison of antimicrobial activity of biosynthesized zinc oxide nanoparticles with Allium jesdianum extract and chemically synthesized
Seyyedeh masumeh Mirnurollahi
1
(
)
رامین محمدی آلوچه
2
(
دانشگاه آزاد اسلامی واحد تهران مرکزی، گروه زیست شناسی
)
Parya Alizadeh-oori
3
(
Department of Microbiology, Faculty of Basic Sciences, Central Tehran Branch,Islamic Azad University,Tehran, Iran
)
Keywords: Green Synthesis, Zinc Oxide Nanoparticles, Allium jesdianum Extract, Streptococcus Iniae, Aeromonas Hydrophila,
Abstract :
Itroduction: Biosynthesis of nanoparticles due to the use of natural agents such as plant extracts and non-toxic chemicals significantly reduce the risk of danger to humans and the ecosystem. Therefore, in this research, the aqueous extract of the Allium jesdianum Extract was used as a reducing and stabilizing agent for the synthesis of zinc oxide nanoparticles, and the chemical synthesis was also carried out with the aim of comparing their antimicrobial activity against Streptococcus Iniae and Aeromonas Hydrophila. Materials and methods: After extracting, nanoparticles were formed by adding 1 to 5 and 1 to 10 concentrations of extracts against 1 mmol zinc oxide solution. Chemical and green synthesized were analyzed with UV-vis, XRD, TEM, FESEM, FTIR and Edax devices and their antimicrobial activity was investigated by disk, well diffusion method and MIC. Results: The production of synthesized nanoparticles was checked by recording the color change with a spectrophotometer in the range of 300-700 nm. Examination with TEM showed that the shape of the spherical particles and their size were 45-115 nm and they had antimicrobial activity against S. Iniae and A. Hydrophila. Conclusion: The results showed that this plant has an antimicrobial effect on Streptococcus iniae. Although chemical nanoparticles showed a better effect, the difference in effect is very small, and since the biosynthesis of nanomaterials has better environmental effects and fewer side effects, green nanoparticles can be a good substitute for chemical nanoparticles and a good candidate for replacing antibiotic therapy.
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