مقایسه فعالیت ضد میکروبی نانوذرات اکسید روی بیوسنتز شده با عصاره گیاه بن سرخ و سنتز شده شیمیایی
سیده معصومه میرنوراللهی
1
(
دانشگاه آزاد اسلامی واحد تهران مرکزی
)
رامین محمدی آلوچه
2
(
دانشگاه آزاد اسلامی واحد تهران مرکزی، گروه زیست شناسی
)
پریا علیزاده عوری
3
(
گروه زیست دانشکده علوم پایه دانشگاه تهران مرکزی
)
الکلمات المفتاحية: سنتز سبز, نانوذرات اکسید روی, عصاره برگ گیاه بن سرخ, استرپتوکوکوس اینیه, آئروموناس هیدروفیلا ,
ملخص المقالة :
مقدمه: بیوسنتز نانوذرات به دليل استفاده از واکنشگرها وعوامل طبيعی نظير عصاره گياهان و مواد شيميایی غيرسمی، ریسک خطرپذیری برای انسان و اکوسيستم را به طور قابل توجهی کاهش می دهند. لذا در این پژوهش، عصاره آبی گیاه بن سرخ به عنوان عامل کاهنده و پایدار کننده جهت سنتز نانوذرات اکسید روی مورد استفاده قرار گرفت و سنتز شیمیایی نیز با هدف مقایسه فعالیت ضد میکروبی آنها بر ضد استرپتوکوکوس اینیه و آئروموناس هیدروفیلا انجام شد.
مواد و روش ها: پس از عصاره گیری، با افزودن غلظت 1 به 5 و 1 به 10 عصاره در برابر محلول اکسید روی mmol1، نانوذرات تشکیل شدند. آنالیز نانوذرات سنتز شده شیمیایی و گیاهی با دستگاه های UV-vis، XRD، TEM، FESEM، FTIR و Edax انجام و فعالیت ضد میکروبی آنها با روش انتشار از دیسک و چاهک وMIC بررسی شد.
نتایج: توليد نانوذرات سنتز شده با ثبت تغيير رنگ با اسپكتوفتومتر در محدودهnm 700-300 بررسی شد. بررسی با TEM نشان داد که شکل ذرات کروی و اندازه آنها nm 115-45 و دارای فعالیت ضد میکروبی بر ضد استرپتوکوکوس اینیه و آئروموناس هیدروفیلا بودند.
نتیجه گیری: نتایج نشان داد این گیاه تاثیر ضد میکروبی بر استرپتوکوکوس اینیه دارد. نانوذرات شیمیایی گر چه اثر بهتری نشان دادند اما تفاوت اثر بسیار ناچیز بوده و از آنجا که بیوسنتز نانومواد اثرات زیست محیطی بهتر و عوارض کمتری دارند، می توان گفت نانوذرات گیاهی می توانند جایگزین مطلوب برای نانوذرات شیمیایی و کاندید مناسبی برای جایگزینی آنتی بیوتیک درمانی در صنعت پرورش ماهی باشند.
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