Prevalence of antibiotic resistance and enterotoxin genes in Staphylococcus aureus producing biofilm isolated from meat and meat products
Subject Areas : Food microbiology
کبری عباسی
1
,
Manouchehr Momeni-Shahraki
2
1 - 1. گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
2 - گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه آزاد شهرکرد، ایران.
Keywords: Staphylococcus aureus, Biofilm, Enterotoxin, Meat,
Abstract :
Staphylococcus aureus is one of the pathogens that can easily infect meat. The present study was performed to evaluate the antimicrobial resistance and distribution of enterotoxin genes in Staphylococcus aureus isolates isolated from raw meat and meat products. 440 samples of raw meat and meat products were collected from suppliers in Shahrekord city and the presence of Staphylococcus aureus was examined using biochemical and molecular tests. The most common enterotoxin genes and genes encoding antibiotic resistance were examined in the presence of specific primers. In this study, out of 440 samples, 61 samples (13.86%) were positive for Staphylococcus aureus. Biofilm reaction was observed in 56 isolates (91.8%). The highest resistance to penicillin (83.60%) and the lowest resistance to vancomycin (0%). The frequency of the presence of sea, seb, sec and sed genes in the studied isolates were reported 39.34%, 27.86%, 26.22% and 37.70%, respectively. Considering that there was a statistically significant relationship between antibiotic resistance genes and biofilm production, it is clear that antibiotic resistance in strains that are able to produce biofilm compared to strains that are not able to produce biofilms are more
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