Studying the effect of drought stress on enzymatic and non-enzymatic antioxidant system of some grape cultivar
Subject Areas : Plant physiologyMojtaba gholizadeh 1 , mehdi Haddadinejad 2 , علی عبادی 3 , Ali Mohammadi Torkashvand 4
1 - Department of Horticultural Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Horticulture of Department, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
3 - استاد،گروه علوم باغبانی، دانشگاه تهران
4 - Professor, Islamic Azad University, Research Sciences Unit, Tehran
Keywords: proline, phenol, catalase, ascorbate-peroxidase ,
Abstract :
In order to investigate the effect of drought stress on the enzymatic and non-enzymatic antioxidant system of six grape varieties, a factorial experiment was conducted based on a completely randomized design with three replications under greenhouse conditions. In this experiment, the treatments included six grape varieties (Asgari, Khalili, Yaquti, Pikami, Turkmen 4 and Suzak) and four levels of drought stress (normal conditions (100% of the farm capacity), low stress (75% of the farm capacity), medium stress (50% of the farm capacity) and severe stress treatment (25% of the farm capacity). The results showed that the traits of proline, malondialdehyde, catalase enzymes and ascorbate peroxidase enzymes improved with increasing stress intensity. increased significantly. On the other hand, the content of total phenol decreased significantly with the increase of the intensity of stress. Among the cultivars studied, Yaqouti cultivar is more resistant to drought than other grape cultivars in terms of the studied indices. The highest amount of catalase enzyme activity was recorded in Yaqouti cultivar, so that its catalase enzyme activity at the levels of 75, 50 and 25% of the agricultural capacity increased by 13, 12 and 46% respectively compared to the 100% agricultural capacity. According to the results of this research, it seems that Yaghuti cultivar is more drought tolerant than other cultivars. Since this toleration mechanisms located in leaf of Yaghuti, it is necessary to carry out additional tests when using as rootstock.
1) اسدی و. رسولی م. غلامی م. و م، ملکی. 1396. بررسی برخی ویژگی¬های ریخت شناختی و فیزیولوژیک چهار رقم انگور (.Vitis vinifera L) در شرایط تنش خشکی. مجله علوم باغبانی ایران، 48(4): 990-997.
2) سوخت¬سرایی، ر.، عبادی، ع.، سلامی، س.ع. و ح، لسانی. 1396. بررسی شاخص¬های اکسیداتیو در سه رقم انگور (Vitis vinifera L.) در شرایط تنش خشکی. مجله علوم باغبانی ایران، 48: 98-85.
3) فهیم، س.، قنبری، ع.ر.، ناجی، ع.م.، شکوهیان، ع.ا. و ح، ملکی لجایر. 1401. تاثیر تنش خشکی روی صفات مورفولوژیکی و فیزیولوژیکی در برخی ارقام انگور ایرانی. فرآیند و کارکرد گیاهی، 11(47)، 249-266.
4) محسنی¬فرد، ا.، نجاتیان، م.ع.، حسینی¬سالکده، ق.، محمدپرست، ب. و ا، مویدی¬نژاد. 1398. تأثیر تنش خشکی بر برخی ویژگی¬های فیزیولوژیکی و بیوشیمیایی دو رقم انگور. فرآیند و کارکردهای گیاهی، 32: 377-390.
5) مهری، ح.ر.، قبادی، س.، بانی¬نسب، ب.، احسان زاده، پ و م، غلامی. 1393. بررسی برخی پاسخ¬های فیزیولوژیک و مورفولوژیک چهار رقم انگور ایرانی (Vitis vinifera L.) به تنش خشکی در شرایط درون شیشه¬ای. فرآیند و کارکرد گیاهی، 10(3)، 115-126.
6) Aebi, H. 1984. Catalase in vitro. Methods Enzymology, 105: 121-126.
7) Altinci, N.T. and R, Cangi, R. 2019. Drought tolerance of some wine grape cultivars under in vitro conditions. Journal of Agricultural Faculty of Gaziosmanpasa University, 36: 145-151.
8) Arora, A., Sairam, R.K. and G.C, Srivastava. 2002. Oxidative stress and antioxidant system in plants. Plant Physiology, 82: 1227-1237.
9) Beis A. and A, Patakas. 2015. Differential physiological and biochemical responses to drought in grapevines subjected to partial root drying and deficit irrigation. European Journal of Agronomy, 62: 90-97.
10) Bertamini, M., Zulini, L., Muthuchelian, K. and N, Nedunchezhian. 2006. Effect of water deficit on phostosynthetic and other physiological responses in grapevine (Vitis vinifera L. cv. Riesling) plants. Photosynthetica, 44: 151-154.
11) Choudhury, F.K., Rivero, R.M., Blumwald, E. and R, Mittler. 2017. Reactive oxygen species, abiotic stress and stress combination. Physiology Plants, 90(5): 856-867.
12) Dacosta, M. and B, Huang. 2007. Changes in antioxidant enzyme activities and lipid pero×idation for bentgrass species in responses to drought stress. Journal of the American Society for Horticultureal Science, 132: 319-326.
13) Esmaeilizadeh, M., Lotfi, A., Mirdehghan, S.H. and M, Shamshiri. 2018. Effects of irrigation intervals on some physiological and biochemical characteristics in four Iranian grapevine cultivars. Crops Improvement, 20: 3-14.
14) Esmaeilizadeh, M., Lotfi, A., Mirdehghan, S.H. and M, Shamshiri. 2018. Effects of irrigation intervals on some physiological and biochemical characteristics in four Iranian grapevine cultivars. Crops Improvement, 20: 3-14.
15) Haider, M.S., Zhang, C. and M.M, Kurjogi. 2017. Insights into grapevine defense response against drought as revealed by biochemical, physiological and RNA-Seq analysis. Scientific reports, 7: 13134.
16) Hasanuzzaman, M., Bhuyan, M.H.M.B., Zulfiqar, F., Raza, A., Mohsin, S.M., Mahmud, J.A., Fujita, M. and V, Fotopoulos. 2020. Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator. Antioxidants (Basel), 9(8): 681.
17) Isfendiyaroglu, M. and E, Zeker. 2002. The relation between phenolic compound and seed dormancy in Pistacia spp. In: AKB E (ed.). 11 Grema Serr Pistachios and Almond. Chieres Optins Mediterraneenes, 232-277.
18) Jalili marandi, R., Hassani, A., Dolati baneh, H., Azizi, H. and R, Haji taghiloo. 2011. Effect of different levels of soil moisture on the morphological and physiological characteristics of three grape cultivars (Vitis vinifera L.). Journal of Horticultural Science, 42: 31-40.
19) Khochert, G. 1987. Carbohydrate determination by phenol- solphoric acid methods. In: Hellebust J. A. and Garigie J. S. (Eds.) pp. 95-97. Handbook of Physiological Methods, Cambridge University Press. UK.
20) Mhamdi, A., Queval, G., Chaouch, S., Vanderauwera, S., Breusegem, F. and G, Noctor. 2010. Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models. Journal of Experimental Botany, 61(15): 4179-4220.
21) Mishra, N., Jiang, Ch., Chen, L., Paul, A., Chatterjee, A. and G, Shen. 2023. Achieving abiotic stress tolerance in plants through antioxidative defense mechanisms. Frontiersin Plant Science, 14: 1110622. doi: 10.3389/fpls.2023.1110622.
22) Mukarram, M., Choudhary, S., Kurjak, D., Petek, A. and M.M.A, Khan. 2021. Drought: Sensing, signalling, effects and tolerance in higher plants. Physiology Plant, 172: 1291–1300.
23) Paquin, R. and P, Lechasseur. 1979. Observations sure one method de dosage de la proline libber dens les extra its de plants. Canadian Journal of Botany, 57: 1851-1854.
24) Ranieri, A., Castagna, J., Pacini, B., Baldan, A. and G.F, Mensuali Sodi. 2003. Soldatini Early production and scavenging of hydrogen peroxide in the apoplast of sunflowers plants exposed to ozone. Journal of Experimental Botany, 54: 2529-2540.
25) Singh, S.K., Sharma, H.S., Datta, S.B. and S.P, Singh. 2000. In vitro growth and leaf compsition of grapevine cultivars as affected by sodium chloride. Biologia Plantarum, 43: 283-286.
26) Sofo A., Dichio B., xiloyannis, C. and A, Masia. 2004. Effects of different irradiance levels on some antioxidant enzymes and on malondialdehyde content during rewatering in olive tree. Plant Science, 166(2): 293-302.
27) Sorori, Sh., Asgharzadeh, A., Marjani, A. and M, Samadi-Kazemi. 2022. Evaluation of Drought Stress Tolerance among some of Grape Cultivars Using Physiological and Biochemical Studies. Journal of Horticultural Science, 36(2): 373-388. http://doi.org/10.22067/JHS.2021.67767.1004
28) Tsugane, K., Kobayarabidopsis shi, K., Niwa, Y., Ohba, Y., Wada, K. and H, Kobayashi. 1999. A recessive Arabidopsis mutant that grows photoautotrophically under salt stress shows enhanced active oxygen detoxification. The Plant Cell, 11: 1195-1206.
29) Wang, F., Zeng, B., Sun, Z. and C, Zhu. 2009. Relationship between prolion and Hg2+ induced oxidative stress in a tolerant rice mutant. Archives of Environmental Contamination and Toxicology, 56(4): 723-731.
