Effect of Eight Weeks of Combined aerobic-resistance Training on Sleep Quality, Prolactin Hormone, and Growth Hormone, of Overweight and obese adolescent girls
Subject Areas : Journal of Physical Activity and HormonesZohreh Nikjo 1 , Mona Mehdizadeh Haghighi 2 , Anahita Shabani 3
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Keywords: Sleep quality, prolactin hormone, growth hormone, combined aerobic-resistance training,
Abstract :
Introduction: This study aimed to determine the effect of eight weeks of combined aerobic resistance training on sleep quality, prolactin hormone, and growth hormone of overweight and obese adolescent girls.
Material & Methods: In this quasi-experimental study, 30 overweight and obese adolescent girls were randomly divided into two experimental groups of 15 people (age: 16.33 ± 0.97 years; body mass index: kg/m2) and control group (age: 16.53±1.18 years; body mass index: 30.48±3.26 kg/m2). The experimental group performed eight weeks of progressive aerobic resistance training, three sessions per week. Each session consists of 10 minutes of warming up, 5 minutes of cooling down, 30 minutes of aerobic exercise with an intensity of 60-80% of the maximum heart rate, and 45 minutes of resistance exercise with an intensity of 50-70% Repetition Maximum (1RM). Before and after eight weeks of training, sleep quality, anxiety level, prolactin, and growth hormones were measured. Statistical information was obtained by one-way ANCOVA and U-man-Whitney tests through SPSS software. A significance level of p<0.05 was considered.
Results: The results showed that eight weeks of aerobic-resistance training had a significant effect on improving sleep quality and anxiety (p=0.01) and had no significant effect on prolactin and growth hormone changes (p>0.05).
Conclusion: Combined aerobic resistance training probably improves the quality of sleep of overweight and obese students and does not affect prolactin and growth hormone levels.
1. Bentham, J., Di Cesare, M., Bilano, V., & Boddy, L. M. (2017). Worldwide trends in children's and adolescents' body mass index, underweight and obesity, in comparison with adults, from 1975 to 2016: a pooled analysis of 2,416 population-based measurement studies with 128.9 million participants. Lancet.
2. Kumar, S., & Kelly, A. S. (Eds.). (2017). Review of childhood obesity: from epidemiology, etiology, and comorbidities to clinical assessment and treatment. Mayo Clinic Proceedings. Elsevier.
3. Ogden, C. L., Carroll, M. D., Kit, B. K., & Flegal, K. M. (2014). Prevalence of childhood and adult obesity in the United States, 2011-2012. JAMA, 311(8), 806-814. DOI: 10.1001/jama.2014.732
4. Guthold, R., Stevens, G. A., Riley, L. M., & Bull, F. C. (2020). Global trends in insufficient physical activity among adolescents: a pooled analysis of 298 population-based surveys with 1.6 million participants. The Lancet Child & Adolescent Health, 4(1), 23-35. DOI: 10.1016/S2352-4642(19)30324-1
5. Chaput, J.-P., & Dutil, C. (2016). Lack of sleep as a contributor to obesity in adolescents: impacts on eating and activity behaviors. International Journal of Behavioral Nutrition and Physical Activity, 13, 1-9. DOI: 10.1186/s12966-016-0370-0
6. Krueger, J. M., Frank, M. G., Wisor, J. P., & Roy, S. (2016). Sleep function: Toward elucidating an enigma. Sleep Medicine Reviews, 28, 46-54. DOI: 10.1016/j.smrv.2015.05.002
7. Dashti, H. S., Jones, S. E., Wood, A. R., Lane, J. M., Van Hees, V. T., Wang, H., et al. (2019). Genome-wide association study identifies genetic loci for self-reported habitual sleep duration supported by accelerometer-derived estimates. Nature Communications, 10(1), 1100. DOI: 10.1038/s41467-019-09025-2
8. Cooper, C. B., Neufeld, E. V., Dolezal, B. A., & Martin, J. L. (2018). Sleep deprivation and obesity in adults: a brief narrative review. BMJ Open Sport & Exercise Medicine, 4(1). DOI: 10.1136/bmjsem-2017-000368
9. Crocq, M.-A. (2017). The history of generalized anxiety disorder as a diagnostic category. Dialogues in Clinical Neuroscience, 19(2), 107-116.
10. Stein, M. B., & Sareen, J. (2015). Generalized anxiety disorder. New England Journal of Medicine, 373(21), 2059-2068. DOI: 10.1056/NEJMcp1503340
11. Roberge, P., Normand-Lauzière, F., Raymond, I., Luc, M., Tanguay-Bernard, M.-M., Duhoux, A., et al. (2015). Generalized anxiety disorder in primary care: mental health services use and treatment adequacy. BMC Family Practice, 16(1), 1-11. DOI: 10.1186/s12875-015-0271-3
12. Alrousan, G., Hassan, A., Pillai, A. A., Atrooz, F., & Salim, S. (2022). Early life sleep deprivation and brain development: Insights from human and animal studies. Frontiers in Neuroscience, 16, 833786. DOI: 10.3389/fnins.2022.833786
13. Lanzi, R., & Tannenbaum, G. S. (1992). Time course and mechanism of growth hormone's negative feedback effect on its own spontaneous release. Endocrinology, 130(2), 780-788. DOI: 10.1210/endo.130.2.780
14. Vahl, N., Jorgensen, J., Skjærbæk, C., Veldhuis, J. D., Orskov, H., & Christiansen, J. S. (1997). Abdominal adiposity rather than age and sex predicts mass and regularity of GH secretion in healthy adults. American Journal of Physiology-Endocrinology and Metabolism, 272(6), E1108-E1116. DOI: 10.1152/ajpendo.1997.272.6.E1108
15. Kanaley, J., Weatherup-Dentes, M., Jaynes, E., & Hartman, M. (1999). Obesity attenuates the growth hormone response to exercise. The Journal of Clinical Endocrinology & Metabolism, 84(9), 3156-3161. DOI: 10.1210/jcem.84.9.6052
16. Torner, L. (2016). Actions of prolactin in the brain: from physiological adaptations to stress and neurogenesis to psychopathology. Frontiers in Endocrinology, 7, 25. DOI: 10.3389/fendo.2016.00025
17. Kraemer, W. J., & Rogol, A. D. (2008). The endocrine system in sports and exercise. John Wiley & Sons.
18. Delgado-Alvarado, M., Tordesillas-Gutierrez, D., Ayesa-Arriola, R., Canal, M., de la Foz, V. O.-G., Labad, J., et al. (2019). Plasma prolactin levels are associated with the severity of illness in drug-naive first-episode psychosis female patients. Archives of Women's Mental Health, 22, 367-373. DOI: 10.1007/s00737-019-00446-4
19. Gupta, S., Mukherjee, A., Biswas, S., Bose, S., Nath, S., & Das, H. N. (2017). Evaluation of endocrine parameters as predictors of major depressive disorder. Indian Journal of Psychological Medicine, 39(6), 766-769. DOI: 10.4103/0253-7176.199627
20. Dhar, A. K., & Barton, D. A. (2016). Depression and the link with cardiovascular disease. Frontiers in Psychiatry, 7, 33. DOI: 10.3389/fpsyt.2016.00033
21. Labad, J., Stojanovic-Pérez, A., Montalvo, I., Solé, M., Cabezas, Á., Ortega, L., et al. (2015). Stress biomarkers as predictors of transition to psychosis in at-risk mental states: roles for cortisol, prolactin, and albumin. Journal of Psychiatric Research, 60, 163-169. DOI: 10.1016/j.jpsychires.2014.10.011
22. Ojike, N., Sowers, J. R., Seixas, A., Ravenell, J., Rodriguez-Figueroa, G., Awadallah, M., et al. (2016). Psychological distress and hypertension: results from the National Health Interview Survey for 2004-2013. Cardiorenal Medicine, 6(3), 198-208. DOI: 10.1159/000447755
23. Nabi, H., Hall, M., Koskenvuo, M., Singh-Manoux, A., Oksanen, T., Suominen, S., et al. (2010). Psychological and somatic symptoms of anxiety and risk of coronary heart disease: the health and social support prospective cohort study. Biological Psychiatry, 67(4), 378-385. DOI: 10.1016/j.biopsych.2009.09.003
24. Yan, L., Wei, J. A., Yang, F., Wang, M., Wang, S., Cheng, T., et al. (2022). Physical exercise prevented stress-induced anxiety via improving brain RNA methylation. Advanced Science, 9(24), 2105731. DOI: 10.1002/advs.202105731
25. Mattson, M. P. (2012). Energy intake and exercise as determinants of brain health and vulnerability to injury and disease. Cell Metabolism, 16(6), 706-722. DOI: 10.1016/j.cmet.2012.10.001
26. Hu, S., Li, X., & Yang, L. (2023). Effects of physical activity in child and adolescent depression and anxiety: role of inflammatory cytokines and stress-related peptide hormones. Frontiers in Neuroscience, 17.
27. Cadegiani, F. A., & Kater, C. E. (2018). Hormonal response to a non-exercise stress test in athletes with overtraining syndrome: Results from the endocrine and metabolic responses on Overtraining syndrome (EROS)—EROS-STRESS. Journal of Science and Medicine in Sport, 21(7), 648-653. DOI: 10.1016/j.jsams.2017.10.013
28. Bettio, L. E., Thacker, J. S., Rodgers, S. P., Brocardo, P. S., Christie, B. R., & Gil-Mohapel, J. (2020). Interplay between hormones and exercise on hippocampal plasticity across the lifespan. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1866(8), 165821. DOI: 10.1016/j.bbadis.2020.165821
29. Richmond, E., & Rogol, A. D. (2016). Endocrine responses to exercise in the developing child and adolescent. Sports Endocrinology, 47, 58-67.
30. Marin, G., Domene, H., Barnes, K. M., Blackwell, B., Cassorla, F., & Cutler, G. R. (1994). The effects of estrogen priming and puberty on the growth hormone response to standardized treadmill exercise and arginine-insulin in normal girls and boys. The Journal of Clinical Endocrinology & Metabolism, 79(2), 537-541. DOI: 10.1210/jcem.79.2.8013190
31. Pomerants, T., Tillmann, V., Karelson, K., Jürimäe, J., & Jürimäe, T. (2008). Impact of acute exercise on bone turnover and growth hormone/insulin-like growth factor axis in boys. Journal of Sports Medicine and Physical Fitness, 48(2), 266.
32. Rejeki, P. S., Pranoto, A., Rahmanto, I., Izzatunnisa, N., Yosika, G. F., Hernaningsih, Y., et al. (2023). The Positive Effect of Four-Week Combined Aerobic–Resistance Training on Body Composition and Adipokine Levels in Obese Females. Sports, 11(4), 90. DOI: 10.3390/sports11040090
33. Alves, J., Saavedra, F., Simão, R., Novaes, J., Rhea, M. R., Green, D., et al. (2012). Does aerobic and strength exercise sequence in the same session affect the oxygen uptake during and post-exercise? The Journal of Strength & Conditioning Research, 26(7), 1872-1878. DOI: 10.1519/JSC.0b013e31823f2f8f
34. Samany, S., & Jokar, B. (2007). Validity and reliability of the short form of depression, anxiety and stress. J Soc Human Sci Shiraz Univ, 26(3).
35. Athanasiou, N., Bogdanis, G. C., & Mastorakos, G. (2023). Endocrine responses of the stress system to different types of exercise. Reviews in Endocrine and Metabolic Disorders, 24(2), 251-266. DOI: 10.1007/s11154-023-09755-5
36. Casazza, K., Hanks, L. J., & Alvarez, J. A. (2010). Role of various cytokines and growth factors in pubertal development. In Cytokines, Growth Mediators and Physical Activity in Children during Puberty (pp. 14-31).
37. Godfrey, R. J., Madgwick, Z., & Whyte, G. P. (2003). The exercise-induced growth hormone response in athletes. Sports Medicine, 33, 599-613. DOI: 10.2165/00007256-200333080-00003
38. Socratis, K., Kostas, D., Athanasios, D., Eleni, D., & Dimitrios, K. (2016). The influence of physical training on blood levels of human growth hormone, testosterone, and procollagen in young rowers. International Journal of Kinesiology and Sports Science, 4(3), 63-69. DOI: 10.7575/aiac.ijkss.v.4n.3p.63
39. Yamaner, F., Kamuk, Y. U., Bayraktaroglu, T., Karacabey, K., Gumus, M., & Akalin, T. C. (2015). The effects of intensive training on selected sex hormones in young wrestlers. The Anthropologist, 20(3), 773-779. DOI: 10.1080/09720073.2015.11891740
40. Eliakim, A., Nemet, D., Zaldivar, F., McMurray, R. G., Culler, F. L., Galassetti, P., et al. (2006). Reduced exercise-associated response of the GH-IGF-I axis and catecholamines in obese children and adolescents. Journal of Applied Physiology, 100(5), 1630-1637. DOI: 10.1152/japplphysiol.01377.2005
41. Ounis, O. B., Elloumi, M., Zouhal, H., Makni, E., Lac, G., Tabka, Z., et al. (2011). Effect of an individualized physical training program on resting cortisol and growth hormone levels and fat oxidation during exercise in obese children. Annales d'Endocrinologie, 72(1), 15-21. DOI: 10.1016/j.ando.2010.11.002
42. Nouri, Y., Mirzaie, B., & Arazi, H. (2013). The effect of resistance and endurance training on resting metabolic rate and body composition in sedentary males. Journal of Zanjan University of Medical Sciences & Health Services, 21(89).
43. Rafiei, M. M., Shavandi, N., Saremi, A., & Shavandi, A. (2014). Comparison of the effects of 6 weeks of resistance training and concurrent training on aerobic power and resting levels of growth hormone and cortisol in healthy children. Journal of Arak University of Medical Sciences, 17(4), 38-46.
44. Draper, P., & Whyte, G. (1997). Anaerobic performance testing.
45. Hackney, A. C., & Saeidi, A. (2019). The thyroid axis, prolactin, and exercise in humans. Current Opinion in Endocrine and Metabolic Research, 9, 45-50. DOI: 10.1016/j.coemr.2019.02.001
46. Hackney, A. C., Davis, H. C., & Lane, A. R. (2015). Exercise augments the nocturnal prolactin rise in exercise-trained men. Therapeutic Advances in Endocrinology and Metabolism, 6(5), 217-222. DOI: 10.1177/2042018815605257
47. Mahmoud Alilou, M., Khanjani, Z., & Bayat, A. (2017). Prediction of anxiety-related emotional disorders in adolescents based on emotion regulation, coping strategies, and symptoms of borderline personality disorder. Clinical Psychology Studies, 7(25), 107-128.
48. Van Cauter, E., Leproult, R., & Plat, L. (2000). Age-related changes in slow wave sleep and REM sleep and relationship with growth hormone and cortisol levels in healthy men. JAMA, 284(7), 861-868. DOI: 10.1001/jama.284.7.861
49. Veldhuis, J. D., Liem, A., South, S., Weltman, A., Weltman, J., Clemmons, D., et al. (1995). Differential impact of age, sex steroid hormones, and obesity on basal versus pulsatile growth hormone secretion in men as assessed in an ultrasensitive chemiluminescence assay. The Journal of Clinical Endocrinology & Metabolism, 80(11), 3209-3222. DOI: 10.1210/jcem.80.11.7595806
50. Chen, L.-J., Fox, K. R., Ku, P.-W., & Chang, Y.-W. (2016). Effects of aquatic exercise on sleep in older adults with mild sleep impairment: a randomized controlled trial. International Journal of Behavioral Medicine, 23, 501-506. DOI: 10.1007/s12529-016-9572-3
51. Oliver, S. R., Rosa, J. S., Minh, T. D., Pontello, A. M., Flores, R. L., Barnett, M., et al. (2010). Dose-dependent relationship between severity of pediatric obesity and blunting of the growth hormone response to exercise. Journal of Applied Physiology, 108(1), 21-27. DOI: 10.1152/japplphysiol.00862.2009
52. Gough, L., Castell, L. M., Gatti, R., & Godfrey, R. J. (2016). Growth hormone concentrations in different body fluids before and after moderate exercise. Sports Medicine-Open, 2, 1-6. DOI: 10.1186/s40798-016-0030-4
53. Gupta, N., Goel, K., Shah, P., & Misra, A. (2012). Childhood obesity in developing countries: epidemiology, determinants, and prevention. Endocrine Reviews, 33(1), 48-70. DOI: 10.1210/er.2011-0001
54. Rojas Vega, S., Hollmann, W., & Strüder, H. K. (2012). Influences of exercise and training on the circulating concentration of prolactin in humans. Journal of Neuroendocrinology, 24(3), 395-402. DOI: 10.1111/j.1365-2826.2011.02279.x
55. Ohara, E., Tokuyama, H., Kitamoto, T., Kitahara, A., Hayashi, A., Hayashi, H., et al. (2017). Laparoscopic sleeve gastrectomy resolves low GHRP-2-stimulated growth hormone levels in obese patients. Obesity Surgery, 27, 2214-2217. DOI: 10.1007/s11695-017-2492-1
56. Amirsasan, R., Sari-Saraf, V., Pourgholi, T., & Armanfar, M. (2015). Comparing the effects of combined endurance-resistance training versus resistance-endurance on growth hormone and insulin-like growth factor-I in non-athlete prepubertal girls. Feyz, Journal of Kashan University of Medical Sciences, 19(3), 214-222.
57. Shamsaei, F., & Cheraghi, F. (2012). Co-morbidity of sleep disorder and general health in the general population of Hamadan. Avicenna Journal of Nursing and Midwifery Care, 20(2), 40-50.
58. Fernandez-Mendoza, J., Calhoun, S. L., Vgontzas, A. N., Li, Y., Gaines, J., Liao, D., et al. (2016). Insomnia phenotypes based on objective sleep duration in adolescents: depression risk and differential behavioral profiles. Brain Sciences, 6(4), 59. DOI: 10.3390/brainsci6040059
59. Bagheri, H., Shahabi, Z., Ebrahimi, H., & Alaeenejad, F. (2007). The association between quality of sleep and health-related quality of life in nurses. Hayat, 12(4), 13-20.
