The teratogenic effects of aqueous extract of Ephedra major on Balb/C mouse embryos third to sixth days of pregnancy

Subject Areas : clinical veterinary science

safoora saffari ¹ , parvin Torabzadeh ² , Ali Karimi ³

1 - Islamic Azad University, karaj
2 - Ph.D. Assistant Professor. Department of Biology, Karaj Branch, Islamic Azad University, karaj, Iran *Coresponding Author: Parvin Torabzadeh [e-mail]
3 - M.Sc. Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran.

Received: 2018-12-12 Accepted : 2019-05-12 Published : 2019-08-23

Keywords: Ephedra Major, Teratogen, Mouse embryo, Abortifacient,

Abstract :

Introduction: Ephedra major plant in traditional medicine is used loss weight, blood pleasure treatment, common cold and muscular weakness. But effects on the body especially during pregnancy has not been reviewed. Therefore in study the teratogenic effects of aqueous extract of Ephedra major on Balb/C mouse embryos third to sixth days of pregnancy has been reviewed. Materials and methods: In this study 50 female Balb/C mice were randomly divided into 6 equal groups a control group (non-injection: 5 mice) and witess (injection of Physiology serum: 5 mice) and 4 experimental groups (each of 10 mice). A lethaldose LD50 was determined in condition of 3086 mg/kg.bw in vivo and selected dose for injection 500, 300 mg/kg.bw. Injection was done on the third to sixth days by enema and mice were sacrificed on fifteenth day of pregnancy. ANOVA statistical method was used and SPSS21 was used for diagramming software and to compare groups of Duncan test was used with significant condition P