Scrutiny Flora and identify the dominant fungal and bacterial rhizosphere microorganisms in the soil fertile productive chitinase and phospholipase enzymes in Iranshahr

Subject Areas :

hadi  hosseini ¹ (Young Researchers Club, Zahedan Branch, Islamic Azad University, Zahedan, Iran)
iman  hormozi ² (Medical Diagnosis Laboratory Expert)
najmeh  shakib ³ (Medical Diagnosis Laboratory Expert)

Received: 2020-07-22 Accepted : 2020-07-22 Published : 2017-07-23

Keywords: Bacteria, Fungi, chitinase, Phospholipase,

Abstract :

The aim of this study was to identify and isolate bacteria and fungi that produce phospholipase and chitinase enzymes in Iranshahr fertile logic soil to identify and produce biological fertilizers for farmers in the province using microorganisms compatible with the geographical conditions of the region (secondary target). Sampling of soil from agricultural fields in fertile areas of Iranshahr city and soil sampling areas were diluted by 10+ 5. For Chitin, Shrimp substation, Sigma Aldrich USA and sterile egg yolk were used for phospholipase substrate. After merging the dedicated substrates bygeneral media culture, positive enzyme microstructures were identified and isolated by halo production. Enzymes were identified and confirmed by spectrophotometry testing and measurement of light absorption at 410 and 280 nm wavelengths. Samples were sent to South Korea's Macrogen Company for molecular identification. The predominant bacteria and fungi producing phospholipase were Bacillus subtilis and Mucor hiemalis, respectivelythe highest production of chitinase enzyme was observed in Pseudomonas putida and Mucor hiemalis microsatellites. Changes in pH showed that an increase in pH to alkalinity greatly reduced enzyme secretion. The highest levels of both enzymes were found in Mucor hiemalis