The Therapeutic Effect of Amniotic Mesenchymal Stem Cell Transplantation in Type-1 Diabetes
Subject Areas : Journal of Animal Biology
Soosan
Rostam Pur
1
(Department of Medical Biotechnology, Iran University of Medical Sciences, Tehran, Iran)
Yadolah
Edalat panah
2
(Cellular and Molecular Research Center, Yasouj University of Medical Sciences, Yasuj, Iran)
Fariba
Enayati parvar
3
(Cellular and Molecular Research Center, Yasouj University of Medical Sciences, Yasuj, Iran)
Reza
Haghighi
4
(Veterinary, Karaj Branch, Islamic Azad University, Karaj, Iran)
Keywords: Stem cell transplantation, Amnion, Type-1 diabetic model, Diabetes treatment,
Abstract :
Diabetes mellitus can occur due to insulin deficiency or environmental tissues resistance to insulin with the reduction of insulin secretion. The present study aimed to investigate the therapeutic effect of amniotic mesenchymal stem cell transplantation in the treatment of type 1 diabetes. This study was of experimental type and the animals were divided into four groups including the control or healthy, the diabetic control group, the group receiving cells with the medium, and the group receiving only supernatant soup. Glucose and blood insulin levels and animal weight were studied by ANOVA and t-test during the study. With the first stage of stem cell transplantation, a significant reduction was found in the animal blood glucose of both groups receiving cell and medium (p = 0.004) and the group receiving supernatant soup (p = 0.014) than the control group. Such results were not observed after the second stage. The insulin levels had no statistically significant difference. The present study indicated that the repeated transplantation of Amniotic Mesenchymal stem cells can decrease the blood glucose but increase the blood insulin level and the injection of supernatant soup alone be solely effective as much as the cell transplantation.
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Zanone M.M., 2010. Human mesenchymal stem cells modulate cellular immune response to islet antigen glutamic acid decarboxylase in type 1 diabetes. The Journal of Clinical Endocrinology Metabolism, 95(8): 3788-3797.
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