Evaluation of the Effects of Methamphetamine Drug on Cell Growth of Stem cells Derived from Uterine Endometrial Tissue of Rats

Subject Areas : Journal of Animal Biology

Zahra Goudarzi ¹ , Seyyed Ebrahim Hosseini ² , ,Davood Mehrabani ³ , Sara Hashemi ⁴

1 - Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2 - 1- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
3 - Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4 - Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Received: 2020-02-20 Accepted : 2020-07-22 Published : 2020-09-15

Keywords: Rat, Stem cell, Endometrium, Methamphetamine, Cell growth,

Abstract :

Methamphetamine is a hallucinogenic substance abused by millions of people all around the world. Cellular toxicity in some cell lines is one of the reported effects of this psychoactive substance. Therefore, this study was conducted to investigate the effect of methamphetamine on the growth of adult mesenchymal stem cells extracted from uterine endometrial tissue in rats. In this experimental study, stem cells were extracted from uterine endometrial tissue in rats, and after cultivation, they were investigated in terms of being mesenchymal using flucytometry method and by CD34, CD90 and CD105 markers. In the third passage of cell culture, the effect of methamphetamine toxicity at a concentration of 0.6 mmol was investigated in a 1-8 day period on the growth of mesenchymal stem cells derived from uterine endometrial tissue by formula PDT= T× . Cells isolated from uterine endometrial tissue adhered completely to the floor of the flask 24 hours after being transferred to the cell culture flask. These cells were positive for non-hematopoietic surface marker expression (CD90, CD105) and negative in terms of hematopoietic marker (CD34) expression, so the mesenchymal identity of these cells was confirmed. The results of the cell count test also showed a significant reduction in the growth of cells treated with 0.6 mmol methamphetamine compared to the control group. The results of this study showed that the cells isolated from the uterine endometrial tissue were of the basic mesenchymal type. The results of cell counting revealed that methamphetamine can inhibit growth by inducing the effects of cell toxicity.

References:

Alavi S.H., Taghavi M.M., Moallem S.A. 2008. Evaluation of effects of methamphetamine repeated dosing on proliferation and apoptosis of rat germ cells. Systems Biology in Reproductive Medicine, 54(2): 85-91.

2. Akhgari M., Mobaraki H., Etemadi-Aleagha A. 2017. Histopathological study of cardiac lesions in methamphetamine poisoning-related deaths. DARU Journal of Pharmaceutical Sciences, 25(1): 5.

3. Astarita G.B., Grimaldi N., Realini Z., Justinova L.V., Panlilio A. 2015. Methamphetamine accelerates cellular senescence through stimulation of de novo ceramide biosynthesis. PloS One, 10(2): e0116961.

4. Baptista S., Lasgi C., Benstaali C., Milhazes N., Borges F., Fontes-Ribeiro  C. 2014. Methamphetamine decreases dentate gyrus stem cell self-renewal and shifts the differentiation towards neuronal fate. Stem Cell Research, 13(2): 329-341.

5. Cadet J.L., Jayanthi M. T., McCoy B., Ladenheim F., Saint-Preux E., Lehrmann S. De. 2013. Genome-wide profiling identifies a subset of methamphetamine (METH)-induced genes associated with METH-induced increased H4K5Ac binding in the rat striatum. BMC Genomics, 14: 545.

6. Cadet J.L., Jayanthi S., Deng X. 2005. Methamphetamine-induced neuronal apoptosis involves the activation of multiple death pathways. Neurotoxicity Research, 8(3-4): 199-206.

7. Campisi J., d'Add  di Fagagna F. 2007. Cellular senescence: when bad things happen to good cells. Nature Reviews Molecular Cell Biology, 8(9): 729-740.

8. Chamberlain G., Fox J., Ashton B., Middleton J. 2007. Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells, 25(11): 2739-2749

9. Castino R., Lazzeri G., Lenzi P., Bellio N., Follo C., Ferrucci M., Fornai F. 2008. Suppression of autophagy precipitates neuronal cell death following low doses of methamphetamine. Journal of Neurochemistry, 106(3): 1426-1439.

10. Conley B.J, CYoung J.,Trounson A.O.,  Mollard R.2004. Derivation, propagation and differentiation of human embryonic stem cells." The international journal of biochemistry & cell biology, 36(4): 555-567.

11.  Fleckenstein A.E., Volz T.J., Riddle E.L., Gibb J.W Hanson G.R. 2007. Mechanism of action of amphetamines. New Insights into the Annual Review of Pharmacology and Toxicology, 47: 681-698.

12. Freund A., Orjalo A.V., Desprez P.Y., Campisi J. 2010. Inflammatory networks during cellular senescence: causes and consequences. Trends Mol Med,  16(5):238-246.

13. Ghobadi F., Rahmanifar F., Mehrabani D., Tamadon, A., Dianatpour M., Zare S., Razeghian Jahromi I. 2018. Endometrial mesenchymal stem stromal cells in mature and immature sheep: An in vitro study.International Journal of Reproductive BioMedicine, 16(2): 83-92.

14. Krasnova I.N., Cadet J.L. 2009. Methamphetamine toxicity and messengers of death. Brain Research Review,  60(2): 379-407.

15.  Mandyam C.D.,  Wee  S., Elena  F., Amelia J.C., Heather N.E.,George  F.R.  2008.Varied access to intravenous methamphetamine self-administration differentially alters adult hippocampal neurogenesis." Biological Psychiatry, 64(11): 958-965.

16. Martin T.A., Jayanthi S., McCoM M.T., Brannock C., Ladenheim B., Garrett T. 2012. Methamphetamine   causes differential alterations in gene expression and patterns of histone acetylation/ hypoacetylation in the rat nucleus accumbens. PLoS One, 7(3): e34236.

17. Meamar R., Dehghani L., Karamali F.2012.Toxicity effects of methamphetamine on embryonic stem cell-derived neuron.. Journal of Research in Medical Sciences. 17(5): 470-474.

18. Mehrabani D., Hassanshahi M., Tamadon A., Zare S., Keshavarz S., Rahmanifar F.2015. Adipose tissue-derived mesenchymal stem cells repair germinal cells of seminiferous tubules of busulfan-induced azoospermic rats.Journalof Human Reproductive Sciences, 8(2): 103-110.

19. Mehrabani D., Rahmanifar F., Mellinejad M., Tamadon A., Dianatpour M., Zare S. F.2015.Isolation culture characterization and adipogenic differentiation of heifer endometrial mesenchymal stem cells. Comparative Clinical Pathology, 24:1159-1164.

20. Mirjalili T., Kalantar S.M., Shams Lahijani M., Sheikhha M.H., Talebi A. 2013. Congenital abnormality effect of methamphetamine on histological, cellular and chromosomal defects in fetal mice..  Iranian Journal of Reproductive Medicine,11(1):39-46.

21. Miyazaki I., Asanuma M., Diaz-Corrales F.J., Fukuda M., Kitaichi K., Miyoshi K. 2006. Methamphetamine-induced dopamin ergic neurotoxicity is regulated by quinone-formation-elatedmolecules. FABES Journal,  20(3): 571-573.

22. Moore T.J., Abrahamse H. 2014. Neuronal Differentiation of Adipose Derived Stem Cells: Progress So Far.International Journal of Photoenergy, 201(6): 1-8.

23. Mullen T.D., Obeid L.M. 2012. Ceramide and apoptosis: exploring the enigmatic connections between sphingolipid metabolism and programmed cell death. Anti-Cancer Agents in Medicinal Chemistry, 12(4):340-363.

24.Papageorgiou M., Raza A., Fraser S., Nurgali K., Apostolopoulos V. 2019. Methamphetamine and its immune-modulating effects. Maturitas,  121:13-21.

25. Schwarzbach V., Lenk K., Laufs U.2020. Methamphetamine‐related cardiovascular diseases. ESC Heart Failure, 7(2): 407-414.

26. Shen K., Zhang Y., Lv X., Chen X., Zhou R., Nguyen L.2016.Molecular Mechanisms Involving Sigma-1 Receptor in Cell Apoptosis of BV-2 Microglial Cells Induced by Methamphetamine. CNS Neurological Disorders Drug Targets, 15(7): 857-865.

27. Smith A. A.2006. glossary for stem-cell biology. Nature, 441(7097): 1060.

28. Tamadon A.,   Mehrabani D.,   Zarezadeh  Y.,   Rahmanifar F.,   Dianatpour M., Zare S.2017.Caprine endometrial mesenchymal stromal stem cell: multilineage potential, characterization, and growth kinetics in breeding and anestrous Stages, Veterinary and Medicine International, 2017: 5052801.

29. Thimios A.M.,  Javier C., Pierfrancesco P., Giovanna O.,  Lucia J.R. 2017. Monitoring notch signaling-associated activation of stem cell niches within injured dental pulp. Frontiers  Physiology, 8: 372.

30. Thanos P.K.,  Kim R.,  Delis F.,  Ananth M.,  Chachati G.,  Rocco, M.J. 2016. Chronic methamphetamine effects on brain structure and function in rats. PLoS One, 11(6): e0155457.

31. Yuan C.J., Quiocho J.M., Kim A., Wee S., Mandyam C.D. 2011. Extended access methamphetamine decreases immature neurons in the hippocampus which results from loss and altered development of neural progenitors without altered dynamics of the S-phase of the cell cycle. Pharmacology Biochemistry and Behavior, 100(1): 98-108.

32. Wang Q., Wei L.W., Xiao H.Q., Xue Y., Du S.H., Liu Y.G. 2017. Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: in vivo and in vitro studies. Food and Chemical Toxicology, 105: 61-72.