Physiological and Histological Studies of Obesity Inducing a High Fat – Diet in Adult NMRI Strain Mice
Subject Areas : Journal of Animal Biology
فرناز
بناکار
1
(دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، گروه زیست شناسی، تهران، ایران)
کاظم
پریور
2
(دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، گروه زیست شناسی، تهران، ایران)
پریچهر
یغمایی
3
(دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، گروه زیست شناسی، تهران، ایران)
هما
محسنی کوچصفهانی
4
(دانشگاه خوارزمی، دانشکده علوم پایه، دانشیار گروه زیست شناسی، تهران، ایران)
Keywords: Obesity, Cholestrol, Triglyceride, NMRI mouse, High fat diet,
Abstract :
Obesity is a major health concern worldwide against which development of new strategies is warranted. Animal models are a valuable tool in these studies and could be developed using a simple and cost effective diet in susceptible strains. Adult NMRI mice weighing 25andplusmn;4 g were fed by a high fat diet for six weeks (E group) in comparison with a control group(C) receiving standard chow. Weekly measurements of body weight were performed. Animals were sacrificed after six weeks, and abdominal fat amount, fat liver accumulation, and LDL-C, HDL-C, cholesterol and triglyceride were measured. Histological studies were done on kidney, liver and testis. Body weight of E group increased up to about 50% (1.25-fold more than C group), their abdominal fat amount showed a two-fold increase compared with the C group(pandlt;0.001), and morphologic differences were observed in liver tissue relative to fat accumulation. Of biochemical factors, only triglyceride levels showed statistically significant increase in the E group (pandlt;0.05). Microscopic assessment of kidney, liver and testis samples, regarding to cells size and number showed no significant differences between C and E groups. This method is proposed as a mean to simulate an early stage of obesity.
