The Effects of Aqueous Extract of Vitex agnus castus and Foeniculum vulgare on HPG axis and Spermatogenesis of Male NMRI Rat
Subject Areas : Journal of Animal Biology
معصومه پیرسلوارانی
1
,
عبدالحسین شیروی
2
,
میترا حیدری نصرآبادی
3
1 - دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران
2 - دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران
3 - دانشگاه آزاد اسلامی، واحد پرند، گروه زیست شناسی، پرند، ایران
Keywords: Foeniculum vulgare, Rat, male, NMRI, Vitex agnus castus,
Abstract :
Vitex-agnus castusandamp; Foeniculum vulgare is without doubt the most important herb used by natural therapists for the treatment of female hormone problems. But there isnandrsquo;t any report about the effect of thisVitex-agnus castus aqueous extract and A. Putting together Vitex-agnus castus and Foeniculum vulgare on serum LH, FSH,Testosteron in male. This project investigates the effects of Vitex-agnus castus and Foeniculum vulgare administration on serum Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH) and Testosterone levels in male NMRI mice. This is an experimental study in which adult male mice were chosen and divided into two groups: control and experimental. The experimental group divided into five groups. Any groups were including eight mice. Animals were daily injected ( i.p.) with 100, 200 mg/kg of foeniculum vulgare aqueous extract and 300, 400 of Vitex agnus aqueous extract and 600 mg/kg of A. putting together Vitex agnus and Foeniculum vulgare aqueous extract for sixteen consecutive days. Then the animals were weighed and eventually, anesthesia by ketamin and xylozine, blood sample were collected from heart, 2 weeks after the last injection. Animalsand#39; blood Serum levels of LH, FSH and testosterone were measured by RIA method. The caudal part of the right epididymis was used for sperm couting. After macroscopic investigation (weight, diameter and volume of testis) tissues were fixed in formalin 10% fixative. Tissues were cut at 5 andmicro;m, stained with Hematoxylin and Eosin (Handamp; E). Ccollected data was analyzed by the SPSS software by using one-way ANOVAand Tukey. No significant differences in body weight, volume, weight and diameter of testis was seen. The effects of Vitex-agnus castus aqueous extract andamp; Foeniculum vulgare aqueous extract, to cause decrease serum Testostern, LH and FSH release compand to controls. Result of sperm count, showed a significant decreasing in 300, 400 doses and compose doses 600 of Vitex and Foeniculum.Vitex-agnus castus andamp;Foeniculum vulgare contains essential fatty acids and estrogenic compound. The results suggest that its extracts may act through the hypothalamo-hypophysel-gonadal axis or direct effect on pituitary.
