Induction of salt tolerant soybean [Glycine max (L) Merr. cv. 033] mutants with gamma ray irradiation under greenhouse condition

Subject Areas : Journal of Plant Ecophysiology

miaad kia ¹ , نادعلی بابائیان جلودار بابائیان جلودار ² , نادعلی باقری ³

1 - مربی مرکز آموزش فنی و حرفه ای
2 - مدیر گروه زراعت و اصلاح نباتات دانشگاه کشاورزی و منابع طبیعی ساری
3 - استادیار گروه اصلاح نباتات دانشگاه کشاورزی ساری

Received: 2014-12-02 Accepted : 2015-04-23 Published : 2016-01-01

Keywords: "soybean", " salinity", " Gamma ray", "tolerance ", "morphological traits,

Abstract :

In order to study salt tolerance of 38 genotypes derived from gamma ray irradiation on 033 soybean cultivar (M2 generation), seeds of selected M1 genotypes were sown under greenhouse condition with 3 levels of sodium chloride (30, 60 and 90 mM) in sandy culture as a factorial experiment on the basis of randomized complete block design with 3 replications in 2008. After 60 days from imposing salinity treatment, the traits of stem length, root length, root node number, plant fresh weight, dry weight of aerial part of plant, root dry weight and plant biomass were recorded. Analysis of variance indicated that there were significant differences between salinity treatments, genotypes and their interaction. Moreover, results showed significant differences in morphological traits. The observations showed the most extensive variation range was belonging to 320 Gy dose of gamma ray. In this study, 13 genotypes were selected and they were tolerant to all salinity levels compare to control. These promising genotypes can be used as new germplasms to obtain new salt tolerant or resistant varieties in near future.