Evaluation of invertase activity in Yeasts isolated from saline soil

Subject Areas : Industrial Microbiology

Sareh  Salehi ¹ (Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran)
Ahmadali  pourbabaei ² (Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran)
Ali  Rezaei ³ (Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran)

Received: 2010-03-16 Accepted : 2010-04-14 Published : 2010-04-01

Keywords: Invertase, Food industries, Halophilic yeasts, Halotolerant yeasts,

Abstract :

Background and objective: Biodiversity of yeasts in extreme habitats and applications in food industry attract many of researchers. The aim of this study was evaluation of invertase activity in isolated yeasts from saline soil. Material and Methods: In this study, kinetic activity of invertase from 27 halophilic and halotolerant yeast strains isolated from salin soil of Qom and Arak were evaluated. After collection of soil samples, the yeasts were cultured on YPD Agar containing 5% salt. Out of 27 yeast strains, 2 halophilic and 6 halotolerant strains with invertase activity were assessed for selection of best strain. Results: Assessment of invertase activity according to the Glucose Oxidase assay showed that the enzyme productivity of G5 strain is more powerful than other isolates. The optimum enzyme activity was obtained at 65°C and pH 4.5. The highest enzymatic activity was observed in the medium containing 1.7mM sodium chloride. Na+, Mg2+ and Ca2+ ions (all chlorides and 5mM concentration) have stimulation effect on enzyme's activity. The Km of enzyme for sucrose was 20 mM. Thermal stability of enzyme in 65°C was one hour and decrease of activity is not observed. Conclusion: According to the results of this study, G5 strain has able to produce invertase in different concentrations of different salts.

References:

1. Alberto F, Bignon C, Sulzenbacher G, Henrissat B. The three dimensional structure of invertase (α-fructosidase) from Thermotoga maritime reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases. J Chem. 2004; 279:18910-18913

2. Ashokkumar B, Kayalvizhi N, Gunasekaran P. Optimization of media for α-fructofuranosidase production by Aspergillus niger in submerged and solid state fermentation. Biochem. 2001; 37:331-338.

3. Vitolo M, Duranti A. Effect of pH, aeration and sucrose feeding on the invertase activity of intact S. cerevisiae cells grown in sugarcane blackstrap molasses. J Indust Microbiol. 2008; 15:75-79.

4. Belcarz A, The novel non−glycosylated invertase from Candida utilis (the properties and the conditions of production and purification). Biochimi Biophys Acta. 2004; 1594:40-53.

5. Chaudhuri A, Maheswari A. novel invertase from a thermophilic fungus Thermomyces lanuginosus: it requirement of protein for activation. Arch Biochem Biophys. 1996; 327:98-106.

6. Moreno Y, Sanchez L, Rodriguez J. Purification and characterizationof the invertase from Schizosaccharomyces pombe. Biochem. 1990; 67:697-702.

7. Rodriguez J, Perez T. Characterization of the invertase from Pichia anomala. Biochem. 1995; 30:235-239.

8. Workman D. Purification and properties of the fructofuranosidase from Kluyveromyces fragilis. Microbiol. 1983; 160:87-91.

9. Moneke S. Physiological properties of yeast cell grown in the culture supplementation media. Agric. Biol.Chem. 1985; 39:1025-1031.

10. Barnett J. Yeast: Characteristics and identification .2^nd ed. Cambridge university. 1990; 1-80.

11. Tulay T, Sezai T. Analysis of the effects of hyperosmotic stress on the derepression of invertase activities and the growth of different baker's yeast strains. Turk. J. Biol. 2002; 26:155-161.

12. Chengchan E. Recovery of yeast invertase from ethanol fermantation broth. Biotechnol. 2008; 14(7):561-576.

13. Rubio M, Navarro A. Invertase from a strain of Rhodotorula glutinis. Phytochem. 2002; 61:605-609.

14. Rubio M, Maldonado, M. Purification and characterization of invertase from Aspergillus niger. Curr. Microbiol. 1995; 31:80-83.

15. Karlekar K, Parekh T, Chhatpar HS. Salt mediated changes in some enzymes of carbohydrate metabolism inhalotolerant Cladosporium sphaerospermum. J. Biosci. 2004; 3(4):197-201.

16. Mona M. Rashad R. Production, purification and characterization of extracellular invertase from Saccharomyses cerevisiae NRRL Y-12632 by solid-state fermentation of red carrot residue. Microbiol. 2009; 3(3):1910-1919.

17. Quiroga E, Vattuone A. Purification and characterization of the invertase in yeast. Biochem. 1995; 35:281-289.

18. Rashad M, Mahmoud M. Purification and characterization of extra and intracellular α-fructofuranosidase from Saccharomyces cerevisiae growing on Eichhornia crassipes leaf extract. Microbiol; 2006; 102:157-166.

19. Nguyen Q, Rezessy M. Purification and some properties invertase in yeast. Bichem. 2005; 59:248-257.

20. Gutierrez A. Biochemical characterization of a β-fructofuranosidase from Rhodotorula dairenensis with transfructosylating activity. Biotechnol. 2009; 35:79-98.

21. Linde D, Macias I. Characterization of a β-fructofuranosidase from Schwanniomyces occidentalis with transfructosylatin activity. Biochem. 2007; 11:354-369.